Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (9): 1855-1863.DOI: 10.1016/S2095-3119(14)60932-1

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Multiphasic characterization of a plant growth promoting bacterial strain, Burkholderia sp. 7016 and its effect on tomato growth in the field

 GAO Miao, ZHOU Jian-jiao, WANG En-tao, CHEN Qian, XU Jing, SUN Jian-guang   

  1. 1、Key Laboratory of Microbial Resources, Ministry of Agriculture/Institute of Agricultural Resources and Regional Planning, Chinese cademy of Agricultural Sciences, Beijing 100081, P.R.China
    2、Departmento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Polytécnico National, Mexico D F 11340,Mexico
  • 收稿日期:2014-09-26 出版日期:2015-09-03 发布日期:2015-09-05
  • 通讯作者: SUN jian-guang, Tel: +86-10-82108701,E-mail: sunjianguang@caas.cn
  • 作者简介:GAO Miao, Tel: +86-10-82108701, E-mail: gaomiao@caas.cn;
  • 基金资助:

    This research was supported by the National Natural Science Foundation of China (31100364) and the National Nonprofit Institute Research Grant of Chinese Academy of Agricultural Sciences (CAAS, IARRP-2014-20).

Multiphasic characterization of a plant growth promoting bacterial strain, Burkholderia sp. 7016 and its effect on tomato growth in the field

 GAO Miao, ZHOU Jian-jiao, WANG En-tao, CHEN Qian, XU Jing, SUN Jian-guang   

  1. 1、Key Laboratory of Microbial Resources, Ministry of Agriculture/Institute of Agricultural Resources and Regional Planning, Chinese cademy of Agricultural Sciences, Beijing 100081, P.R.China
    2、Departmento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Polytécnico National, Mexico D F 11340,Mexico
  • Received:2014-09-26 Online:2015-09-03 Published:2015-09-05
  • Contact: SUN jian-guang, Tel: +86-10-82108701,E-mail: sunjianguang@caas.cn
  • About author:GAO Miao, Tel: +86-10-82108701, E-mail: gaomiao@caas.cn;
  • Supported by:

    This research was supported by the National Natural Science Foundation of China (31100364) and the National Nonprofit Institute Research Grant of Chinese Academy of Agricultural Sciences (CAAS, IARRP-2014-20).

摘要: Aiming at searching for plant growth promoting rhizobacteria (PGPR), a bacterium strain coded as 7016 was isolated from soybean rhizosphere and was characterized in the present study. It was identified as Burkholderia sp. based on 16S rDNA sequence analysis, as well as phenotypic and biochemical characterizations. This bacterium presented nitrogenase activity, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity and phosphate solubilizing ability; inhibited the growth of Sclerotinia sclerotiorum, Gibberella zeae and Verticillium dahliae; and produced small quantities of indole acetic acid (IAA). In green house experiments, significant increases in shoot height and weight, root length and weight, and stem diameter were observed on tomato plants in 30 d after inoculation with strain 7016. Result of 16S rDNA PCR-DGGE showed that 7016 survived in the rhizosphere of tomato seedlings. In the field experiments, Burkholderia sp. 7016 enhanced the tomato yield and significantly promoted activities of soil urease, phosphatase, sucrase, and catalase. All these results demonstrated Burkholderia sp. 7016 as a valuable PGPR and a candidate of biofertilizer.

关键词: PGPR , Burkholderia sp. , multiphasic characterization , promoting growth , biofertilizer

Abstract: Aiming at searching for plant growth promoting rhizobacteria (PGPR), a bacterium strain coded as 7016 was isolated from soybean rhizosphere and was characterized in the present study. It was identified as Burkholderia sp. based on 16S rDNA sequence analysis, as well as phenotypic and biochemical characterizations. This bacterium presented nitrogenase activity, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity and phosphate solubilizing ability; inhibited the growth of Sclerotinia sclerotiorum, Gibberella zeae and Verticillium dahliae; and produced small quantities of indole acetic acid (IAA). In green house experiments, significant increases in shoot height and weight, root length and weight, and stem diameter were observed on tomato plants in 30 d after inoculation with strain 7016. Result of 16S rDNA PCR-DGGE showed that 7016 survived in the rhizosphere of tomato seedlings. In the field experiments, Burkholderia sp. 7016 enhanced the tomato yield and significantly promoted activities of soil urease, phosphatase, sucrase, and catalase. All these results demonstrated Burkholderia sp. 7016 as a valuable PGPR and a candidate of biofertilizer.

Key words: PGPR , Burkholderia sp. , multiphasic characterization , promoting growth , biofertilizer