Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (11): 2445-2451.DOI: 10.1016/S2095-3119(13)60609-7

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Antagonizing Aspergillus parasiticus and Promoting Peanut Growth of Bacillus Isolated from Peanut Geocarposphere Soil

 XIAO Wei, YAN Pei-sheng, WU Han-qi and LIN Feng   

  1. School of Marine Science and Technology, Harbin Institute of Technology, Weihai 264209, P.R.China
  • 收稿日期:2013-06-09 出版日期:2014-11-06 发布日期:2014-11-14
  • 通讯作者: YAN Pei-sheng, E-mail: psyan6@hotmail.com
  • 作者简介:XIAO Wei, E-mail: tuerxiao@yeah.net
  • 基金资助:

    This study was supported by the National Natural Science Foundation of China (30571244, 30870003), and the National Key Technology R&D Program of China (2009BADA0B05-4).

Antagonizing Aspergillus parasiticus and Promoting Peanut Growth of Bacillus Isolated from Peanut Geocarposphere Soil

 XIAO Wei, YAN Pei-sheng, WU Han-qi and LIN Feng   

  1. School of Marine Science and Technology, Harbin Institute of Technology, Weihai 264209, P.R.China
  • Received:2013-06-09 Online:2014-11-06 Published:2014-11-14
  • Contact: YAN Pei-sheng, E-mail: psyan6@hotmail.com
  • About author:XIAO Wei, E-mail: tuerxiao@yeah.net
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (30571244, 30870003), and the National Key Technology R&D Program of China (2009BADA0B05-4).

摘要: A set of 38 Bacillus strains isolated from peanut geocarposphere soil were screened as potential biological control agent anti- Aspergillus parasiticus. Tip-culture method with rapid and quantitative characteristics was used to determine anti-A. parasiticus activity and the process of isolation could be accelerated with this method. 12 out of 38 Bacillus strains showed high anti-aflatoxin production activity. These 12 Bacillus strains were selected to identify the characteristics of promoting peanuts germination rate. Pot experiment in greenhouse was carried out by using these strains which can promote peanuts germination. Phytohormones in the fermentation broth were also detected as another important reference factor to select the isolates as biological control agent with PGPR features. These Bacillus strains isolated from peanut geocarposphere soil not only had high ability anti-Aspergillus parasiticus, but also promoted peanut growth. Therefore, these Bacillus strains were well adapted to peanut production in the field as biological control agent with plant growth promoting rhizobacteria (PGPR) features.

关键词: Bacillus strains , anti-Aspergillus parasiticus , PGPR features , peanut

Abstract: A set of 38 Bacillus strains isolated from peanut geocarposphere soil were screened as potential biological control agent anti- Aspergillus parasiticus. Tip-culture method with rapid and quantitative characteristics was used to determine anti-A. parasiticus activity and the process of isolation could be accelerated with this method. 12 out of 38 Bacillus strains showed high anti-aflatoxin production activity. These 12 Bacillus strains were selected to identify the characteristics of promoting peanuts germination rate. Pot experiment in greenhouse was carried out by using these strains which can promote peanuts germination. Phytohormones in the fermentation broth were also detected as another important reference factor to select the isolates as biological control agent with PGPR features. These Bacillus strains isolated from peanut geocarposphere soil not only had high ability anti-Aspergillus parasiticus, but also promoted peanut growth. Therefore, these Bacillus strains were well adapted to peanut production in the field as biological control agent with plant growth promoting rhizobacteria (PGPR) features.

Key words: Bacillus strains , anti-Aspergillus parasiticus , PGPR features , peanut