Novel SNP of EPAS1 gene associated with higher hemoglobin concentration revealed the hypoxia adaptation of yak (Bos grunniens)

doi:10.1016/S2095-3119(14)60854-6

Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (4): 741-748.DOI: 10.1016/S2095-3119(14)60854-6

Novel SNP of EPAS1 gene associated with higher hemoglobin concentration revealed the hypoxia adaptation of yak (Bos grunniens)

WU Xiao-yun, DING Xue-zhi, CHU Min, GUO Xian, BAO Peng-jia, LIANG Chun-nian, YAN Ping

Key Laboratory for Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, P.R.China

收稿日期:2014-04-29 出版日期:2015-04-01 发布日期:2015-04-10
通讯作者: YAN Ping, Tel/Fax: +86-931-2115271,E-mail: pingyanlz@163.com; LIANG Chun-nian, Tel: +86-931-2115271, E-mail: chunnian2006@163.com
作者简介:WU Xiao-yun, E-mail: wxiaoyunkevin@gmail.com;
基金资助:
Research supported by the Special Fund for Agro-scientific Research in the Public Interest, China (201003061), the Key Technologies R&D Program of China during the 12th Five-Year Plan period (2012BAD13B05), the Great Project of Science and Technology of Gansu Province in China (1102NKDA027), and the National Natural Science Foundation of China (31101702).

Novel SNP of EPAS1 gene associated with higher hemoglobin concentration revealed the hypoxia adaptation of yak (Bos grunniens)

WU Xiao-yun, DING Xue-zhi, CHU Min, GUO Xian, BAO Peng-jia, LIANG Chun-nian, YAN Ping

Key Laboratory for Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, P.R.China

Received:2014-04-29 Online:2015-04-01 Published:2015-04-10
Contact: YAN Ping, Tel/Fax: +86-931-2115271,E-mail: pingyanlz@163.com; LIANG Chun-nian, Tel: +86-931-2115271, E-mail: chunnian2006@163.com
About author:WU Xiao-yun, E-mail: wxiaoyunkevin@gmail.com;
Supported by:
Research supported by the Special Fund for Agro-scientific Research in the Public Interest, China (201003061), the Key Technologies R&D Program of China during the 12th Five-Year Plan period (2012BAD13B05), the Great Project of Science and Technology of Gansu Province in China (1102NKDA027), and the National Natural Science Foundation of China (31101702).

摘要/Abstract

摘要： Endothelial PAS domain protein 1 gene (EPAS1) is a key transcription factor that activates the expression of oxygen-regulated genes. In this study, in order to better understand the effects of EPAS1 gene on hematologic parameters in yak, we firstly quantified the tissue expression patterns for EPAS1 mRNA of yak, identified polymorphism in this gene and evaluated its association with hematologic parameters. Expression of EPAS1 mRNA was detected in all eight tissues (heart, liver, lung, spleen, pancreas, kidney, muscles and ovary). The expressions of EPAS1 in lung and pancreas were extremely higher than other tissues examined. Three novel single nucleotide polymorphisms (SNPs) (g.83052 C>T, g.83065 G>A and g.83067 C>A) within the EPAS1 were identified and genotyped in Pali (PL), Gannan (GN) and Tianzhu White (TZW) yak breeds. Significant higher frequencies of the AA and GA genotypes and A allele of the g.83065 G>A were observed in the PL and GN breeds than that in the TZW breed (P<0.01). Association analysis of the PL breed indicated that the g.83065 G>A polymorphism was significantly associated with hemoglobin (HGB) concentration in yaks (P<0.05). Individuals with genotype AA had significantly higher HGB concentration (P<0.05) than those with genotype GA and GG. All these results will help our further understanding of biological functional of yak EPAS1 gene in responding to hypoxia and also indicate EPAS1 might contribute to the hypoxia adaptation of the yak.

关键词: EPAS1 gene , mRNA expression , polymorphism , hypoxia adaptation , yak

Abstract: Endothelial PAS domain protein 1 gene (EPAS1) is a key transcription factor that activates the expression of oxygen-regulated genes. In this study, in order to better understand the effects of EPAS1 gene on hematologic parameters in yak, we firstly quantified the tissue expression patterns for EPAS1 mRNA of yak, identified polymorphism in this gene and evaluated its association with hematologic parameters. Expression of EPAS1 mRNA was detected in all eight tissues (heart, liver, lung, spleen, pancreas, kidney, muscles and ovary). The expressions of EPAS1 in lung and pancreas were extremely higher than other tissues examined. Three novel single nucleotide polymorphisms (SNPs) (g.83052 C>T, g.83065 G>A and g.83067 C>A) within the EPAS1 were identified and genotyped in Pali (PL), Gannan (GN) and Tianzhu White (TZW) yak breeds. Significant higher frequencies of the AA and GA genotypes and A allele of the g.83065 G>A were observed in the PL and GN breeds than that in the TZW breed (P<0.01). Association analysis of the PL breed indicated that the g.83065 G>A polymorphism was significantly associated with hemoglobin (HGB) concentration in yaks (P<0.05). Individuals with genotype AA had significantly higher HGB concentration (P<0.05) than those with genotype GA and GG. All these results will help our further understanding of biological functional of yak EPAS1 gene in responding to hypoxia and also indicate EPAS1 might contribute to the hypoxia adaptation of the yak.

Key words: EPAS1 gene , mRNA expression , polymorphism , hypoxia adaptation , yak

WU Xiao-yun, DING Xue-zhi, CHU Min, GUO Xian, BAO Peng-jia, LIANG Chun-nian, YAN Ping. Novel SNP of EPAS1 gene associated with higher hemoglobin concentration revealed the hypoxia adaptation of yak (Bos grunniens)[J]. Journal of Integrative Agriculture, 2015, 14(4): 741-748.

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Novel SNP of EPAS1 gene associated with higher hemoglobin concentration revealed the hypoxia adaptation of yak (Bos grunniens)

Novel SNP of EPAS1 gene associated with higher hemoglobin concentration revealed the hypoxia adaptation of yak (Bos grunniens)

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