Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (4): 698-705.DOI: 10.1016/S2095-3119(14)60822-4

• 论文 • 上一篇    下一篇

Cloning and RNA interference analysis of the salivary protein C002 gene in Schizaphis graminum

 ZHANG Yong, FAN Jia, SUN Jing-rui, CHEN Ju-lian   

  1. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
  • 收稿日期:2014-02-17 出版日期:2015-04-01 发布日期:2015-04-10
  • 通讯作者: CHEN Ju-lian, Tel: +86-10-62813685,E-mail: jlchen@ippcaas.cn
  • 作者简介:ZHANG Yong, E-mail: zhangyongnky@163.com;
  • 基金资助:

    The project was supported by the National Natural Science Foundation of China (30971920, 31371946), the International Cooperation Project between China and Belgium (2010DFA32810) and the Earmarked Fund for Modern Agro-Industry Technology Research System, China (CARS- 3).

Cloning and RNA interference analysis of the salivary protein C002 gene in Schizaphis graminum

 ZHANG Yong, FAN Jia, SUN Jing-rui, CHEN Ju-lian   

  1. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
  • Received:2014-02-17 Online:2015-04-01 Published:2015-04-10
  • Contact: CHEN Ju-lian, Tel: +86-10-62813685,E-mail: jlchen@ippcaas.cn
  • About author:ZHANG Yong, E-mail: zhangyongnky@163.com;
  • Supported by:

    The project was supported by the National Natural Science Foundation of China (30971920, 31371946), the International Cooperation Project between China and Belgium (2010DFA32810) and the Earmarked Fund for Modern Agro-Industry Technology Research System, China (CARS- 3).

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摘要： The full-length cDNA of functionally-unknown salivary protein C002 in Schizaphis graminum was cloned using rapid amplification of cDNA ends (RACE) and designated as SgC002 (GenBank accession no. KC977563). It is 767 bp long and encodes a protein of 190 amino acid residues with a predicted mass of 21.5 kDa and a predicted cleavage site of N-terminal signal peptide between the 24th and the 25th residues. SgC002 is specifically expressed in salivary gland with the highest level at the 2nd instar. Introducing SgC002-specific 476-siRNA, but not 546-siRNA to aphids through artificial diet significantly suppressed SgC002 expression. Silencing SgC002 gene led to lethality of the aphid on wheat plants, but not on pure artificial diet. Our study demonstrated that artificial diet-mediated RNAi can be a useful tool for research on the roles of genes in aphid salivary gland, and also provided new insights into the characteristics of C002 in wheat aphids.

关键词: Schizaphis graminum , salivary protein C002 , cDNA clone , siRNA

Abstract: The full-length cDNA of functionally-unknown salivary protein C002 in Schizaphis graminum was cloned using rapid amplification of cDNA ends (RACE) and designated as SgC002 (GenBank accession no. KC977563). It is 767 bp long and encodes a protein of 190 amino acid residues with a predicted mass of 21.5 kDa and a predicted cleavage site of N-terminal signal peptide between the 24th and the 25th residues. SgC002 is specifically expressed in salivary gland with the highest level at the 2nd instar. Introducing SgC002-specific 476-siRNA, but not 546-siRNA to aphids through artificial diet significantly suppressed SgC002 expression. Silencing SgC002 gene led to lethality of the aphid on wheat plants, but not on pure artificial diet. Our study demonstrated that artificial diet-mediated RNAi can be a useful tool for research on the roles of genes in aphid salivary gland, and also provided new insights into the characteristics of C002 in wheat aphids.

Key words: Schizaphis graminum , salivary protein C002 , cDNA clone , siRNA