Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (1): 88-97.DOI: 10.1016/S2095-3119(14)60795-4

• 论文 • 上一篇    下一篇

Allelopathy of decomposed maize straw products on three soilborn diseases of wheat and the analysis by GC-MS

 QI Yong-zhi, ZHEN Wen-chao, LI Hai-yan   

  1. College of Plant Protection, Agricultural University of Hebei, Baoding 071001, P.R.China
  • 收稿日期:2014-01-03 出版日期:2015-01-01 发布日期:2015-01-08
  • 通讯作者: ZHEN Wen-chao, Tel: +86-312-7528158, E-mail: wenchao@hebau.edu.cn
  • 作者简介:QI Yong-zhi, E-mail: qiyongzhi1981@163.com;
  • 基金资助:

    This research was financially supported by the Key Technologies R&D Program of China during the 12th Five- Year Plan period (2011BAD16B08, 2012BAD04B06 and 2013BAD07B05).

Allelopathy of decomposed maize straw products on three soilborn diseases of wheat and the analysis by GC-MS

 QI Yong-zhi, ZHEN Wen-chao, LI Hai-yan   

  1. College of Plant Protection, Agricultural University of Hebei, Baoding 071001, P.R.China
  • Received:2014-01-03 Online:2015-01-01 Published:2015-01-08
  • Contact: ZHEN Wen-chao, Tel: +86-312-7528158, E-mail: wenchao@hebau.edu.cn
  • About author:QI Yong-zhi, E-mail: qiyongzhi1981@163.com;
  • Supported by:

    This research was financially supported by the Key Technologies R&D Program of China during the 12th Five- Year Plan period (2011BAD16B08, 2012BAD04B06 and 2013BAD07B05).

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摘要： In northern China, the soil-born diseases of wheat have been getting more and more serious under a new farming system that returns maize straw to the field. In order to investigate the allelopathy of the decomposed maize straw products on three soil-born diseases of wheat, culture dish and pot experiments were conducted and the compounds in the products were identified by gas chromatography-mass spectrometry (GC-MS). Culture dish experiments showed that the mycelial growth, sclerotia formation amount and total weight of Rhizoctonia cerealis were promoted at concentrations of 0.03, 0.06 and 0.12 g mL–1 and inhibited at concentration of 0.48 g mL–1 of the decomposed products. No significant effects were found of the product concentrations on average weight of the sclerotia. Mycelial growth of Gaeumannomyces graminis was promoted at almost all concentrations except the highest one. Mycelial growth and spore germination of Bipolaris sorokiniana were significantly inhibited by all concentrations of the decomposed products, with enhanced inhibition effects along with the increased concentrations. The length, number and dry weight of roots together with the root superoxide dismutase activity were promoted by the lowest concentration (0.03 g mL–1), with a synthetic effect index of 0.012, and inhibited by other concentrations. The ion leakage of roots was increased and the root peroxidase activity of roots was lowered by all the treatments. Pot experiments revealed that occurrence of the sharp eyespot was reduced by 0.03 and 0.06 g mL–1 of decomposed products after irrigation. However, the incidence rates and disease indexes were significantly increased by 0.12, 0.24 and 0.48 g mL–1 of decomposed products. The results indicated that incidence rates and disease indexes of the take-all were significantly promoted after being irrigated with the decomposed products, while occurrences of the common rot didn’t change, significantly. GC-MS results showed that the compounds of the decomposed products included organic acids, esters, hydrocarbons, amides and aldehydes, with the proportions 25.26, 24.01, 17.22, 14.39 and 7.73%, respectively. Further analysis investigated that the allelochemicals identified in straw decomposed products contained p-hydroxybenzoic acid (9.21%), dibutyl phthalate (6.94%), 3-phenyl-2-acrylic (5.06%), 4-hydroxy-3,5-dimethoxybenzoic acid (2.26%), hexanoic acid (1.73%), 8-octadecenoic acid (1.06%), 3-(4-hydroxy-3-methoxy-phenyl)-2-propenoic acid (1.04%), 4-hydroxy-3-methoxy-benzoic acid (0.94%) and salicylic acid (0.94%).

关键词: wheat , soil-born disease , maize straw , decomposed products , allelopathy , GC-MS

Abstract: In northern China, the soil-born diseases of wheat have been getting more and more serious under a new farming system that returns maize straw to the field. In order to investigate the allelopathy of the decomposed maize straw products on three soil-born diseases of wheat, culture dish and pot experiments were conducted and the compounds in the products were identified by gas chromatography-mass spectrometry (GC-MS). Culture dish experiments showed that the mycelial growth, sclerotia formation amount and total weight of Rhizoctonia cerealis were promoted at concentrations of 0.03, 0.06 and 0.12 g mL–1 and inhibited at concentration of 0.48 g mL–1 of the decomposed products. No significant effects were found of the product concentrations on average weight of the sclerotia. Mycelial growth of Gaeumannomyces graminis was promoted at almost all concentrations except the highest one. Mycelial growth and spore germination of Bipolaris sorokiniana were significantly inhibited by all concentrations of the decomposed products, with enhanced inhibition effects along with the increased concentrations. The length, number and dry weight of roots together with the root superoxide dismutase activity were promoted by the lowest concentration (0.03 g mL–1), with a synthetic effect index of 0.012, and inhibited by other concentrations. The ion leakage of roots was increased and the root peroxidase activity of roots was lowered by all the treatments. Pot experiments revealed that occurrence of the sharp eyespot was reduced by 0.03 and 0.06 g mL–1 of decomposed products after irrigation. However, the incidence rates and disease indexes were significantly increased by 0.12, 0.24 and 0.48 g mL–1 of decomposed products. The results indicated that incidence rates and disease indexes of the take-all were significantly promoted after being irrigated with the decomposed products, while occurrences of the common rot didn’t change, significantly. GC-MS results showed that the compounds of the decomposed products included organic acids, esters, hydrocarbons, amides and aldehydes, with the proportions 25.26, 24.01, 17.22, 14.39 and 7.73%, respectively. Further analysis investigated that the allelochemicals identified in straw decomposed products contained p-hydroxybenzoic acid (9.21%), dibutyl phthalate (6.94%), 3-phenyl-2-acrylic (5.06%), 4-hydroxy-3,5-dimethoxybenzoic acid (2.26%), hexanoic acid (1.73%), 8-octadecenoic acid (1.06%), 3-(4-hydroxy-3-methoxy-phenyl)-2-propenoic acid (1.04%), 4-hydroxy-3-methoxy-benzoic acid (0.94%) and salicylic acid (0.94%).

Key words: wheat , soil-born disease , maize straw , decomposed products , allelopathy , GC-MS