Effects of root restriction on nitrogen and gene expression levels in nitrogen metabolism in Jumeigui grapevines (Vitis vinifera L.×Vitis labrusca L.)

doi:10.1016/S2095-3119(14)60876-5

Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (1): 67-79.DOI: 10.1016/S2095-3119(14)60876-5

Effects of root restriction on nitrogen and gene expression levels in nitrogen metabolism in Jumeigui grapevines (Vitis vinifera L.×Vitis labrusca L.)

YU Xiu-ming, LI Jie-fa, ZHU Li-na, WANG Bo, WANG Lei, BAI Yang, ZHANG Cai-xi, XU Wen-ping, WANG Shi-ping

1、Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, P.R.China
2、Shanghai Academy of Agricultural Sciences, Shanghai 201106, P.R.China
3、Guangxi Academy of Agricultural Sciences, Nanning 530007, P.R.China

收稿日期:2014-03-06 出版日期:2015-01-01 发布日期:2015-01-08
通讯作者: WANG Shi-ping, Tel/Fax: +86-21-34205956,E-mail: fruit@sjtu.edu.cn
作者简介:YU Xiu-ming, E-mail: yuxiumingfish@sjtu.edu.cn;
基金资助:
This work was supported by the National Natural Science Foundation of China (30871695). The authors thank Dr. Cao Xiongjun (Guangxi Academy of Agricultural Sciences, China) for access to field assistance.

Effects of root restriction on nitrogen and gene expression levels in nitrogen metabolism in Jumeigui grapevines (Vitis vinifera L.×Vitis labrusca L.)

YU Xiu-ming, LI Jie-fa, ZHU Li-na, WANG Bo, WANG Lei, BAI Yang, ZHANG Cai-xi, XU Wen-ping, WANG Shi-ping

1、Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, P.R.China
2、Shanghai Academy of Agricultural Sciences, Shanghai 201106, P.R.China
3、Guangxi Academy of Agricultural Sciences, Nanning 530007, P.R.China

Received:2014-03-06 Online:2015-01-01 Published:2015-01-08
Contact: WANG Shi-ping, Tel/Fax: +86-21-34205956,E-mail: fruit@sjtu.edu.cn
About author:YU Xiu-ming, E-mail: yuxiumingfish@sjtu.edu.cn;
Supported by:
This work was supported by the National Natural Science Foundation of China (30871695). The authors thank Dr. Cao Xiongjun (Guangxi Academy of Agricultural Sciences, China) for access to field assistance.

摘要/Abstract

摘要： To decipher the relationship between the inhibited shoot growth and expression pattern of key enzymes in nitrogen metabolism under root restriction, the effects of root restriction on diurnal variation of expression of nitrate reductase (NR), nitrite reductase (NiR), glutamine synthetase (GS1-1, GS1-2, GS2) and glutamate synthase (Fd-GOGAT, NADH-GOGAT) genes and nitrogen levels were evaluated in two-year-old Jumeigui grapevines (Vitis vinifera L.×Vitis labrusca L.) when significant differences in shoot growth were observed between treatments at expansion stage (22 days after anthesis). Grapevines were planted in root-restricting pits as root restriction and in an unrestricted field as the control. Results showed that root restriction significantly reduced shoot growth, but promoted the growth of white roots and fibrous brown roots and improved the fruit quality. (NO3 –+NO2 –)-N concentration in all plant parts, NH4 +-N concentration in white roots and total N concentration in leaves and brown roots were significantly reduced under root restriction. Gene expression analysis revealed that mRNA levels of genes related to the GS1/NADH-GOGAT pathway were lower in root-restricted than in control petioles, whereas genes involved in the GS2/Fd-GOGAT pathway were up-regulated under root restriction. Root restriction also resulted in downregulation of genes involved in nitrogen metabolism in leaves, especially at 10:00, while transcript levels of all these genes were enhanced in root-restricted white and brown roots at most time points. This organ-dependent response contributed to the alteration in NO3 – reduction and NH4 + assimilation under root restriction, leading to less NO3 – transported from roots and then assimilated in root-restricted leaves. Therefore, this study implied that shoot growth inhibition in grapevines under root restriction is closely associated with down-regulation of gene expression in nitrogen metabolism in leaves.

关键词: root restriction , grapevine , gene expression , nitrogen metabolism

Abstract: To decipher the relationship between the inhibited shoot growth and expression pattern of key enzymes in nitrogen metabolism under root restriction, the effects of root restriction on diurnal variation of expression of nitrate reductase (NR), nitrite reductase (NiR), glutamine synthetase (GS1-1, GS1-2, GS2) and glutamate synthase (Fd-GOGAT, NADH-GOGAT) genes and nitrogen levels were evaluated in two-year-old Jumeigui grapevines (Vitis vinifera L.×Vitis labrusca L.) when significant differences in shoot growth were observed between treatments at expansion stage (22 days after anthesis). Grapevines were planted in root-restricting pits as root restriction and in an unrestricted field as the control. Results showed that root restriction significantly reduced shoot growth, but promoted the growth of white roots and fibrous brown roots and improved the fruit quality. (NO3 –+NO2 –)-N concentration in all plant parts, NH4 +-N concentration in white roots and total N concentration in leaves and brown roots were significantly reduced under root restriction. Gene expression analysis revealed that mRNA levels of genes related to the GS1/NADH-GOGAT pathway were lower in root-restricted than in control petioles, whereas genes involved in the GS2/Fd-GOGAT pathway were up-regulated under root restriction. Root restriction also resulted in downregulation of genes involved in nitrogen metabolism in leaves, especially at 10:00, while transcript levels of all these genes were enhanced in root-restricted white and brown roots at most time points. This organ-dependent response contributed to the alteration in NO3 – reduction and NH4 + assimilation under root restriction, leading to less NO3 – transported from roots and then assimilated in root-restricted leaves. Therefore, this study implied that shoot growth inhibition in grapevines under root restriction is closely associated with down-regulation of gene expression in nitrogen metabolism in leaves.

Key words: root restriction , grapevine , gene expression , nitrogen metabolism

YU Xiu-ming, LI Jie-fa, ZHU Li-na, WANG Bo, WANG Lei, BAI Yang, ZHANG Cai-xi, XU Wen-ping, WANG Shi-ping. Effects of root restriction on nitrogen and gene expression levels in nitrogen metabolism in Jumeigui grapevines (Vitis vinifera L.×Vitis labrusca L.)[J]. Journal of Integrative Agriculture, 2015, 14(1): 67-79.

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Effects of root restriction on nitrogen and gene expression levels in nitrogen metabolism in Jumeigui grapevines (Vitis vinifera L.×Vitis labrusca L.)

Effects of root restriction on nitrogen and gene expression levels in nitrogen metabolism in Jumeigui grapevines (Vitis vinifera L.×Vitis labrusca L.)

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