Molecular Characterization and Functional Analysis of Krüppel-homolog 1 (Kr-h1) in the Brown Planthopper, Nilaparvata lugens (Stål)

doi:10.1016/S2095-3119(13)60654-1

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (9): 1972-1981.DOI: 10.1016/S2095-3119(13)60654-1

Molecular Characterization and Functional Analysis of Krüppel-homolog 1 (Kr-h1) in the Brown Planthopper, Nilaparvata lugens (Stål)

JIN Min-na, XUE Jian, YAO Yun , LIN Xin-da

1、College of Life Sciences, China Jiliang University, Hangzhou 310018, P.R.China
2、Institute of Insect Science, Zhejiang University, Hangzhou 310058, P.R.China

收稿日期:2013-05-10 出版日期:2014-09-22 发布日期:2014-09-24
通讯作者: LIN Xinda,Tel: +86-571-86835772, E-mail: 07a0903045@cjlu.edu.cn
作者简介:JIN Min-na, Tel: +86-571-86835772, E-mail: 1007101022@cjlu.edu.cn; XUE Jian, Tel: +86-571-88982991, E-mail: alexjxue@gmail.com
基金资助:
This work was supported by the National 973 Program of China (2010CB126205) and the Zhejiang Provincial Natural Science Foundation of China (LY14C140002).

Molecular Characterization and Functional Analysis of Krüppel-homolog 1 (Kr-h1) in the Brown Planthopper, Nilaparvata lugens (Stål)

JIN Min-na, XUE Jian, YAO Yun , LIN Xin-da

1、College of Life Sciences, China Jiliang University, Hangzhou 310018, P.R.China
2、Institute of Insect Science, Zhejiang University, Hangzhou 310058, P.R.China

Received:2013-05-10 Online:2014-09-22 Published:2014-09-24
Contact: LIN Xinda,Tel: +86-571-86835772, E-mail: 07a0903045@cjlu.edu.cn
About author:JIN Min-na, Tel: +86-571-86835772, E-mail: 1007101022@cjlu.edu.cn; XUE Jian, Tel: +86-571-88982991, E-mail: alexjxue@gmail.com
Supported by:
This work was supported by the National 973 Program of China (2010CB126205) and the Zhejiang Provincial Natural Science Foundation of China (LY14C140002).

摘要/Abstract

摘要： The brown planthopper, Nilaparvata lugens (Stål), is the most serious insect pest of rice. It has developed high resistance to traditional insecticides because of their intensive use. Juvenile hormone (JH) analogs have been used successfully to control this species and other pest insects. However, the molecular mechanism of JH signaling is not well understood. Krüppel-homolog 1 (Kr-h1) is a transcription factor involved in the JH pathway. In this study, the Kr-h1 cDNA was cloned and characterized from N. lugens by rapid amplification of cDNA ends (RACE) and reverse transcription PCR (RT-PCR). Its spatial and temporal expression profiles were examined by real-time quantitative PCR, and its function was also studied by RNA interference (RNAi). The open reading frame of NlKr-h1 is 1 833 bp encoding for 611 amino acids. The protein contains eight conserved zinc-finger motifs. NlKr-h1 was expressed at all life stages, with the highest mRNA level in the 4-day embryo. NlKr-h1 mRNA levels rose during each nymphal molt after the 2nd instar. In the adults, the mRNA level in males was significantly higher than that in females of either the macropterous or brachypterous type. The highest expression was observed in the female midgut. NlKr-h1 was activated by juvenile hormone III (JH III) in the 3rd-5th instar nymphs. Disruption of Nlkr-h1 expression by RNAi caused stunted wing development and malformations of both male and female external genitalia. Our findings suggest that Kr-h1 may be a useful target for pest insect management.

关键词: brown planthopper , Krü, ppel-homolog 1 , RNA interference , juvenile hormone signaling pathway

Abstract: The brown planthopper, Nilaparvata lugens (Stål), is the most serious insect pest of rice. It has developed high resistance to traditional insecticides because of their intensive use. Juvenile hormone (JH) analogs have been used successfully to control this species and other pest insects. However, the molecular mechanism of JH signaling is not well understood. Krüppel-homolog 1 (Kr-h1) is a transcription factor involved in the JH pathway. In this study, the Kr-h1 cDNA was cloned and characterized from N. lugens by rapid amplification of cDNA ends (RACE) and reverse transcription PCR (RT-PCR). Its spatial and temporal expression profiles were examined by real-time quantitative PCR, and its function was also studied by RNA interference (RNAi). The open reading frame of NlKr-h1 is 1 833 bp encoding for 611 amino acids. The protein contains eight conserved zinc-finger motifs. NlKr-h1 was expressed at all life stages, with the highest mRNA level in the 4-day embryo. NlKr-h1 mRNA levels rose during each nymphal molt after the 2nd instar. In the adults, the mRNA level in males was significantly higher than that in females of either the macropterous or brachypterous type. The highest expression was observed in the female midgut. NlKr-h1 was activated by juvenile hormone III (JH III) in the 3rd-5th instar nymphs. Disruption of Nlkr-h1 expression by RNAi caused stunted wing development and malformations of both male and female external genitalia. Our findings suggest that Kr-h1 may be a useful target for pest insect management.

Key words: brown planthopper , Krüppel-homolog 1 , RNA interference , juvenile hormone signaling pathway

JIN Min-na, XUE Jian, YAO Yun , LIN Xin-da. Molecular Characterization and Functional Analysis of Krüppel-homolog 1 (Kr-h1) in the Brown Planthopper, Nilaparvata lugens (Stål)[J]. Journal of Integrative Agriculture, 2014, 13(9): 1972-1981.

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Molecular Characterization and Functional Analysis of Krüppel-homolog 1 (Kr-h1) in the Brown Planthopper, Nilaparvata lugens (Stål)

Molecular Characterization and Functional Analysis of Krüppel-homolog 1 (Kr-h1) in the Brown Planthopper, Nilaparvata lugens (Stål)

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