Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (4): 911-917.DOI: 10.1016/S2095-3119(13)60312-3

• 论文 • 上一篇    

Antimicrobial Susceptibility and Characterization of Outer Membrane Proteins of Aeromonas hydrophila Isolated in China

 GUO Peng, WANG Na, LIU Yong-jie , LU Cheng-ping   

  1. 1、College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P.R.China
    2、Institute of Animal Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100029, P.R.China
  • 收稿日期:2012-11-01 出版日期:2014-04-01 发布日期:2014-04-16
  • 通讯作者: LIU Yong-jie, Tel/Fax: +86-25-84398606, E-mail: liuyongjie@njau.edu.cn
  • 作者简介:GUO Peng, E-mail: apachegp@gmail.com
  • 基金资助:

    This work was supported by the National Natural Science Foundation of China (31072151), the Specialized Research Fund for the Doctoral Program of Higher Education, China (20090097110007) and the Priority Academic Program Development of Jiangsu Higher Education Institutions, China (PAPD).

Antimicrobial Susceptibility and Characterization of Outer Membrane Proteins of Aeromonas hydrophila Isolated in China

 GUO Peng, WANG Na, LIU Yong-jie , LU Cheng-ping   

  1. 1、College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P.R.China
    2、Institute of Animal Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100029, P.R.China
  • Received:2012-11-01 Online:2014-04-01 Published:2014-04-16
  • Contact: LIU Yong-jie, Tel/Fax: +86-25-84398606, E-mail: liuyongjie@njau.edu.cn
  • About author:GUO Peng, E-mail: apachegp@gmail.com
  • Supported by:

    This work was supported by the National Natural Science Foundation of China (31072151), the Specialized Research Fund for the Doctoral Program of Higher Education, China (20090097110007) and the Priority Academic Program Development of Jiangsu Higher Education Institutions, China (PAPD).

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摘要： Aeromonas hydrophila isolates from clinical cases (n=43) were tested against 8 antimicrobial agents and typed by outer membrane protein (OMP) pattern by using sodium dodecyl sulfate gel electrophoresis. All isolates were resistant to ampicillin (MICs, 16 mg mL-1) and sulfamonomethoxine (MICs, 64 mg mL-1), but susceptible to norfloxacin (MICs, 0.5 mg mL-1). There was a high incidence of resistance to erythromycin (90.70%) and tylosin (93.02%), while a low incidences of resistance to ciprofloxacin (2.33%), enrofloxacin (2.33%) and florfenicol (4.65%). Six different outer membrane protein patterns were found among 34 isolates by analyzing proteins in the range of 22 to 50 kDa, other than 9 isolates with their respective profiles. The strains with the similar OMP profiles had similar resistances. Compared with the other strains from the same OMP patterns, NB-1, A.Pun and MR-1 had lacked the proteins in the range of 30 to 45 kDa and their resistance to florfenicol substantially increased. It is speculated that the outer membrane protein changes might correlate with decreased susceptibility to florfenicol in the three strains. Some strains which showed completely identical OMP types had a little difference in their resistance to fluoroquinolones, indicating that there might be other factors that were involved in the antimicrobial resistance of A. hydrophila.

关键词: Aeromonas hydrophila , antimicrobial resistance , outer membrane proteins

Abstract: Aeromonas hydrophila isolates from clinical cases (n=43) were tested against 8 antimicrobial agents and typed by outer membrane protein (OMP) pattern by using sodium dodecyl sulfate gel electrophoresis. All isolates were resistant to ampicillin (MICs, 16 mg mL-1) and sulfamonomethoxine (MICs, 64 mg mL-1), but susceptible to norfloxacin (MICs, 0.5 mg mL-1). There was a high incidence of resistance to erythromycin (90.70%) and tylosin (93.02%), while a low incidences of resistance to ciprofloxacin (2.33%), enrofloxacin (2.33%) and florfenicol (4.65%). Six different outer membrane protein patterns were found among 34 isolates by analyzing proteins in the range of 22 to 50 kDa, other than 9 isolates with their respective profiles. The strains with the similar OMP profiles had similar resistances. Compared with the other strains from the same OMP patterns, NB-1, A.Pun and MR-1 had lacked the proteins in the range of 30 to 45 kDa and their resistance to florfenicol substantially increased. It is speculated that the outer membrane protein changes might correlate with decreased susceptibility to florfenicol in the three strains. Some strains which showed completely identical OMP types had a little difference in their resistance to fluoroquinolones, indicating that there might be other factors that were involved in the antimicrobial resistance of A. hydrophila.

Key words: Aeromonas hydrophila , antimicrobial resistance , outer membrane proteins