Immunoproteomic Analysis of Bordetella bronchiseptica Outer Membrane Proteins and Identification of New Immunogenic Proteins

doi:10.1016/S2095-3119(13)60618-8

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (9): 2010-2018.DOI: 10.1016/S2095-3119(13)60618-8

Immunoproteomic Analysis of Bordetella bronchiseptica Outer Membrane Proteins and Identification of New Immunogenic Proteins

LIU Yan, QIN Feng-yan, BAO Guo-lian, CHEN Hui, XIAO Chen-wen, WEI Qiang , JI Quan-an

Animal Husbandry and Veterinary Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, P.R.China

收稿日期:2012-11-01 出版日期:2014-09-22 发布日期:2014-09-24
通讯作者: BAO Guo-lian, Tel: +86-571-86404215, Fax: +86-571-86400836, E-mail: baoguolian@163.com
作者简介:LIU Yan, Mobile: 13136184186, E-mail: ly-liuyan@163.com
基金资助:
This work was supported by the China Agricultural Research System (nycytx-44-3-2), the Zhejiang Key Project on Agricultural Development through Science and Technology (2011C12028), the National Natural Science Foundation ofChina (31302068) and the Zhejiang Provincial Natural Science Foundation of China (LQ13C180002).

Immunoproteomic Analysis of Bordetella bronchiseptica Outer Membrane Proteins and Identification of New Immunogenic Proteins

LIU Yan, QIN Feng-yan, BAO Guo-lian, CHEN Hui, XIAO Chen-wen, WEI Qiang , JI Quan-an

Animal Husbandry and Veterinary Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, P.R.China

Received:2012-11-01 Online:2014-09-22 Published:2014-09-24
Contact: BAO Guo-lian, Tel: +86-571-86404215, Fax: +86-571-86400836, E-mail: baoguolian@163.com
About author:LIU Yan, Mobile: 13136184186, E-mail: ly-liuyan@163.com
Supported by:
This work was supported by the China Agricultural Research System (nycytx-44-3-2), the Zhejiang Key Project on Agricultural Development through Science and Technology (2011C12028), the National Natural Science Foundation ofChina (31302068) and the Zhejiang Provincial Natural Science Foundation of China (LQ13C180002).

摘要/Abstract

摘要： Bordetella bronchiseptica is a Gram-negative pathogen that causes acute and chronic respiratory infection in a variety of animals. To identify useful antigen candidates for diagnosis and subunit vaccine of B. bronchiseptica, immunoproteomic analysis was adopted to analyse outer membrane proteins of it. The outer membrane proteins extracted from B. bronchiseptica were separated by two-dimensional gel electrophoresis and analyzed by Western blotting for their reactivity with the convalescent serum against two strains. Immunogenic proteins were identified by matrix-assisted laser desorption/ionization time of flight-mass spectrometry (MALDI-TOF-MS), a total of 14 proteins are common immunoreactive proteins, of which 1 was known antigen and 13 were novel immunogenic proteins for B. bronchiseptica. Putative lipoprotein gene was cloned and recombinantly expressed. The recombinant protein induced high titer antibody, but showed low protective indices against challenges with HB (B. bronchiseptica strain isolated from a infected rabbit). The mortality of mice was 80% compared to 100% of positive controls. The identification of these novel antigenic proteins is an important resource for further development of a new diagnostic test and vaccine for B. bronchiseptica.

关键词: Bordetella bronchiseptica , outer membrane proteins , immunoproteomic analysis

Abstract: Bordetella bronchiseptica is a Gram-negative pathogen that causes acute and chronic respiratory infection in a variety of animals. To identify useful antigen candidates for diagnosis and subunit vaccine of B. bronchiseptica, immunoproteomic analysis was adopted to analyse outer membrane proteins of it. The outer membrane proteins extracted from B. bronchiseptica were separated by two-dimensional gel electrophoresis and analyzed by Western blotting for their reactivity with the convalescent serum against two strains. Immunogenic proteins were identified by matrix-assisted laser desorption/ionization time of flight-mass spectrometry (MALDI-TOF-MS), a total of 14 proteins are common immunoreactive proteins, of which 1 was known antigen and 13 were novel immunogenic proteins for B. bronchiseptica. Putative lipoprotein gene was cloned and recombinantly expressed. The recombinant protein induced high titer antibody, but showed low protective indices against challenges with HB (B. bronchiseptica strain isolated from a infected rabbit). The mortality of mice was 80% compared to 100% of positive controls. The identification of these novel antigenic proteins is an important resource for further development of a new diagnostic test and vaccine for B. bronchiseptica.

Key words: Bordetella bronchiseptica , outer membrane proteins , immunoproteomic analysis

LIU Yan, QIN Feng-yan, BAO Guo-lian, CHEN Hui, XIAO Chen-wen, WEI Qiang , JI Quan-an. Immunoproteomic Analysis of Bordetella bronchiseptica Outer Membrane Proteins and Identification of New Immunogenic Proteins[J]. Journal of Integrative Agriculture, 2014, 13(9): 2010-2018.

参考文献

Bernardini G, Braconi D, Lusini P, Santucci A. 2007. Helicobacter pylori immunoproteomics related to different pathologies. Expert Review of Proteomics, 4, 679-689

Cainelli Gebara V C, Risoléo L, Lopes A P, Ferreira V R, Quintilio W, Lépine F, Silva W D, Raw I 2007. Adjuvant and immunogenic activities of the 73kDa N-terminal alpha-domain of BrkA autotransporter and Cpn60/60kDa chaperonin of Bordetella pertussis. Vaccine, 25, 621-629

Campodónico V L, Llosa N J, Bentancor L V, Maira-Litran T, Pier G B. 2011. Efficacy of a conjugate vaccine containing polymannuronic acid and flagellin against experimental Pseudomonas aeruginosa lung infection in mice. Infection and Immunity, 79, 3455-3464

Del Giudice G, Gervaix A, Costantino P, Wyler C A, Tougne C, de Graeff-Meeder E R, van Embden J, van der Zee R, Nencioni L, Rappuoli R. 1993. Priming to heat shock proteins in infants vaccinated against pertussis. Journal of Immunology, 150, 2025-2032

Glass L S, Beasley J N. 1989. Infection with and antibody response to Pasteurella multocida and Bordetella bronchiseptica in immature rabbits. Laboratory Animal Science, 49, 668-672

Gopinathan L, Kirimanjeswara G S, Wolfe D N, Kelley M L, Harvill E T. 2007. Different mechanisms of vaccine- induced and infection-induced immunity to Bordetella bronchiseptica. Microbes and Infection, 9, 442-448

Henningham A, Chiarot E, Gillen C M, Cole J N, Rohde M, Fulde M, Ramachandran V, Cork A J, Hartas J, Magor G, Djordjevic S P, Cordwell S J, Kobe B, Sriprakash K S, Nizet V, Chhatwal G S, Margarit I Y, Batzloff M R, Walker M J. 2012. Conserved anchorless surface proteins as group A streptococcal vaccine candidates. Journal of Molecular Medicine, 90, 1197-1207

Hong S H, Byun Y H, Nguyen C T, Kim S Y, Seong B L, Park S, Woo G J, Yoon Y, Koh J T, Fujihashi K, Rhee J H, Lee S E 2012. Intranasal administration of a flagellin- adjuvanted inactivated influenza vaccine enhances mucosal immune responses to protect mice against lethal infection. Vaccine, 30, 466-474

Koebnik R, Locher K P, van Gelder P. 2000. Structure and function of bacterial outer membrane proteins: barrels in a nutshell. Molecular Microbiology, 37, 39-53

Kurupati P, Teh B K, Kumarasinghe G, Poh C L. 2006. Identification of vaccine candidate antigens of an ESBL producing Klebsiella pneumoniae clinical strain by immunoproteome analysis. Proteomics, 6, 836-844

Lin J, Huang S, Zhang Q. 2002. Outer membrane proteins: key players for bacterial adaptation in host niches. Microbes and Infection, 4, 325-331

Long G H, Sinha D, Read A F, Pritt S, Kline B, Harvill E T, Hudson P J, Bjørnstad O N. 2010. Identifying the age cohort responsible for transmission in a natural outbreak of Bordetella bronchiseptica. PLOS Pathogens, 6, e1001224.

López-Boado Y S, Cobb L M, Deora R. 2005. Bordetella bronchiseptica flagellin is a proinflammatory determinant for airway epithelial cells. Infection and Immunity, 73, 7525-7534

Mattoo S, Cherry J D. 2005. Molecular pathogenesis, epidemiology, and clinical manifestations of respiratory infections due to Bordetella pertussis and other Bordetella subspecies. Clinical Microbiology Reviews, 18, 326-382

Molloy M P, Herbert B R, Walsh B J, Tyler M I, Traini M, Sanchez J C, Hochstrasser D F, Williams K L, Gooley A A. 1998. Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis. Electrophoresis, 19, 837-844

Oh E, Becker A H, Sandikci A, Huber D, Chaba R, Gloge F, Nichols R J, Typas A, Gross C A, Kramer G, Weissman J S, Bukau B. 2011. Selective ribosome profiling reveals the cotranslational chaperone action of trigger factor in vivo. Cell, 147, 1295-1308

Pang H Y, Li Y, Wu Z H, Jian J C, Lu Y S, Cai S H. 2010. Immunoproteomic analysis and identification of novel immunogenic proteins from Vibrio harveyi. Journal of Applied Microbiology, 109, 1800-1809

Peng X, Ye X, Wang S. 2004. Identification of novel immunogenic proteins of Shigella flexneri 2a by proteomic methodologies. Vaccine, 22, 2750-2756

Rodríguez-Ortega M J, Norais N, Bensi G, Liberatori S, Capo S, Mora M, Scarselli M, Doro F, Ferrari G, Garaguso I, Maggi T, Neumann A, Covre A, Telford J L, Grandi G. 2006. Characterization and identification of vaccine candidate proteins through analysis of the group A Streptococcus surface proteome. Nature Biotechnology, 24, 191-197

Sebaihia M, Preston A, Maskell D J, Kuzmiak H, Connell T D, King N D, Orndorff P E, Miyamoto D M, Thomson N R, Harris D, Goble A, Lord A, Murphy L, Quail M A, Rutter S, Squares R, Squares S, Woodward J, Parkhill J, Temple L M. 2006. Comparison of the genome sequence of the poultry pathogen Bordetella avium with those of B. bronchiseptica, B. pertussis, and B. parapertussis reveals extensive diversity in surface structures associated with host interaction. Journal of Bacteriology, 188, 6002- 6015.

Tefon B E, Maass S, Ozcengiz E, Becher D, Hecker M, Ozcengiz G. 2011. A comprehensive analysis of Bordetella pertussis surface proteome and identification of new immunogenic proteins. Vaccine, 29, 3583-3595

Teixeira-Gomes A P, Cloeckaert A, Bézard G, Bowden R A, Dubray G, Zygmunt M S 1997. Identification and characterization of Brucella ovis immunogenic proteins using two-dimensional electrophoresis and immunoblotting. Electrophoresis, 18, 1491-1497

Yevsa T, Ebensen T, Fuchs B, Zygmunt B, Libanova R, Gross R, Schulze K, Guzmán C A. 2012. Development and characterization of attenuated metabolic mutants of Bordetella bronchiseptica for applications in vaccinology. Environmental Microbiology, 15, 64-76

Ying T, Wang H, Li M, Wang J, Wang J, Shi Z, Feng E, Liu X, Su G, Wei K, Zhang X, Huang P, Huang L. 2005. Immunoproteomics of outer membrane proteins and extracellular proteins of Shigella flexneri 2a 2457T. Proteomics, 5, 4777-4793

Zhang S, Xu Y, Zhou Z, Wang S, Yang R, Wang J, Wang L. 2011. Complete genome sequence of Bordetella pertussis CS, a Chinese pertussis vaccine strain. Journal of Bacteriology, 193, 4017-4018

Zhou M, Guo Y, Zhao J, Hu Q, Hu Y, Zhang A, Chen H, Jin M. 2009. Identification and characterization of novel immunogenic outer membrane proteins of Haemophilus parasuis serovar 5. Vaccine, 27, 5271-5277

Zhu Y Z, Cai C S, Zhang W, Guo H X, Zhang J P, Ji Y Y, Ma G Y, Wu J L, Li Q T, Lu C P, Guo X K. 2010. Immunoproteomic analysis of human serological antibody responses to vaccination with whole-cell pertussis vaccine (WCV). PLOS One, 5, e13915.

Immunoproteomic Analysis of Bordetella bronchiseptica Outer Membrane Proteins and Identification of New Immunogenic Proteins

Immunoproteomic Analysis of Bordetella bronchiseptica Outer Membrane Proteins and Identification of New Immunogenic Proteins

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