Isolation and Expression Analysis of Two Genes Encoding Cinnamate 4-Hydroxylase from Cotton (Gossypium hirsutum)

doi:10.1016/S2095-3119(13)60643-7

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (10): 2102-2112.DOI: 10.1016/S2095-3119(13)60643-7

Isolation and Expression Analysis of Two Genes Encoding Cinnamate 4-Hydroxylase from Cotton (Gossypium hirsutum)

NI Zhi-yong, LI Bo, Neumann M Peter, LÜ Meng , FAN Ling

1、Institute of Nuclear and Biological Technologies, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, P.R.China
2、College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, P.R.China
3、Plant Physiology Laboratory, Department of Environmental, Water and Agricultural Engineering, Faculty of Civil and Environmental
Engineering, Technion-Israel Institute of Technology, Haifa 32000, Israel

收稿日期:2013-06-26 出版日期:2014-10-01 发布日期:2014-10-10
通讯作者: FAN Ling, Tel/Fax: +86-991-4527003, E-mail: fanling@xaas.ac.cn
作者简介:NI Zhi-yong, E-mail: nizhiyong@126.com;
基金资助:
This work was funded by the National Natural Science Foundation of China (31060173), the Joint Funds of the National Natural Science Foundation of China (U1178305) and the High-Tech R&D Program of Xinjiang, China (201111116).

Isolation and Expression Analysis of Two Genes Encoding Cinnamate 4-Hydroxylase from Cotton (Gossypium hirsutum)

NI Zhi-yong, LI Bo, Neumann M Peter, LÜ Meng , FAN Ling

1、Institute of Nuclear and Biological Technologies, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, P.R.China
2、College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, P.R.China
3、Plant Physiology Laboratory, Department of Environmental, Water and Agricultural Engineering, Faculty of Civil and Environmental
Engineering, Technion-Israel Institute of Technology, Haifa 32000, Israel

Received:2013-06-26 Online:2014-10-01 Published:2014-10-10
Contact: FAN Ling, Tel/Fax: +86-991-4527003, E-mail: fanling@xaas.ac.cn
About author:NI Zhi-yong, E-mail: nizhiyong@126.com;
Supported by:
This work was funded by the National Natural Science Foundation of China (31060173), the Joint Funds of the National Natural Science Foundation of China (U1178305) and the High-Tech R&D Program of Xinjiang, China (201111116).

摘要/Abstract

摘要： Two genes (GhC4H1 and GhC4H2) that encode putative cotton cinnamate 4-hydroxylases that catalyze the second step in the phenylpropanoid pathway were isolated from developing cotton fibers. GhC4H1 and GhC4H2 each contain open reading frames of 1 518 base pairs (bp) in length and both encode proteins consisting of 505 amino acid residues. They are 90.89% identical to each other at the amino acid sequence level and belong to class I of plant C4Hs. GhC4H1 and GhC4H2 genomic DNA are 2 247 and 2 161 bp long, respectively, and contain two introns located at conserved positions relative to the coding sequence. GhC4H1 and GhC4H2 promoters were isolated and found to contain many cis-elements (boxes P, L and AC-I element) previously identified in the promoters of other phenylpropanoid pathway genes. Histochemical staining showed GUS expression driven by the GhC4H1 and GhC4H2 promoters in ovules and fibers tissues. GhC4H1 and GhC4H2 were also widely expressed in other cotton tissues. GhC4H2 expression reached its highest level during the elongation stage of fiber development, whereas GhC4H1 expression increased during the secondary wall development period in cotton fibers. Our results contribute to a better understanding of the biochemical role of GhC4H1 and GhC4H2 in cotton fiber development.

关键词: cinnamate 4-hydroxylase , Gossypium hirsutum , promoter analysis , phenylpropanoid pathway

Abstract: Two genes (GhC4H1 and GhC4H2) that encode putative cotton cinnamate 4-hydroxylases that catalyze the second step in the phenylpropanoid pathway were isolated from developing cotton fibers. GhC4H1 and GhC4H2 each contain open reading frames of 1 518 base pairs (bp) in length and both encode proteins consisting of 505 amino acid residues. They are 90.89% identical to each other at the amino acid sequence level and belong to class I of plant C4Hs. GhC4H1 and GhC4H2 genomic DNA are 2 247 and 2 161 bp long, respectively, and contain two introns located at conserved positions relative to the coding sequence. GhC4H1 and GhC4H2 promoters were isolated and found to contain many cis-elements (boxes P, L and AC-I element) previously identified in the promoters of other phenylpropanoid pathway genes. Histochemical staining showed GUS expression driven by the GhC4H1 and GhC4H2 promoters in ovules and fibers tissues. GhC4H1 and GhC4H2 were also widely expressed in other cotton tissues. GhC4H2 expression reached its highest level during the elongation stage of fiber development, whereas GhC4H1 expression increased during the secondary wall development period in cotton fibers. Our results contribute to a better understanding of the biochemical role of GhC4H1 and GhC4H2 in cotton fiber development.

Key words: cinnamate 4-hydroxylase , Gossypium hirsutum , promoter analysis , phenylpropanoid pathway

NI Zhi-yong, LI Bo, Neumann M Peter, Lü Meng , FAN Ling. Isolation and Expression Analysis of Two Genes Encoding Cinnamate 4-Hydroxylase from Cotton (Gossypium hirsutum)[J]. Journal of Integrative Agriculture, 2014, 13(10): 2102-2112.

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Isolation and Expression Analysis of Two Genes Encoding Cinnamate 4-Hydroxylase from Cotton (Gossypium hirsutum)

Isolation and Expression Analysis of Two Genes Encoding Cinnamate 4-Hydroxylase from Cotton (Gossypium hirsutum)

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