Cloning and Characterization of a Somatic Embryogenesis Receptor-Like Kinase Gene in Cotton (Gossypium hirsutum)

doi:10.1016/S1671-2927(00)8612

Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (6): 898-909.DOI: 10.1016/S1671-2927(00)8612

Cloning and Characterization of a Somatic Embryogenesis Receptor-Like Kinase Gene in Cotton (Gossypium hirsutum)

SHI Ya-li, WU Xiao-ping, MENG Zhi-gang, GUO San-dui

Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

收稿日期:2011-02-16 出版日期:2012-06-01 发布日期:2012-07-20
通讯作者: Correspondence GUO San-dui, Tel/Fax: +86-10-82106140, E-mail: gsdui@mail.caas.net.cn
基金资助:
This work was supported by the Research Initiative of Development of Transgenic Cotton Plants funded by Ministry of Agriculture, China (2008ZX08005-004).

Cloning and Characterization of a Somatic Embryogenesis Receptor-Like Kinase Gene in Cotton (Gossypium hirsutum)

SHI Ya-li, WU Xiao-ping, MENG Zhi-gang, GUO San-dui

Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

Received:2011-02-16 Online:2012-06-01 Published:2012-07-20
Contact: Correspondence GUO San-dui, Tel/Fax: +86-10-82106140, E-mail: gsdui@mail.caas.net.cn
Supported by:
This work was supported by the Research Initiative of Development of Transgenic Cotton Plants funded by Ministry of Agriculture, China (2008ZX08005-004).

摘要/Abstract

摘要： A novel gene, GhSERK1, was identified in cotton. It encoded a protein belonging to the somatic embryogenesis receptorlike kinase (SERK) family. The genomic sequence of GhSERK1 was 6 920 bp in length, containing a predicted transcriptional start site (TSS). Its full-length cDNA was 2 502 bp, encoding a protein of 627 amino acids. Sequence analysis of GhSERK1 revealed high levels of similarity to other reported SERKs, as well as a conserved intron/exon structure that was unique to members of the SERK family. Expression analysis showed that GhSERK1 mRNA was present in all organs of cotton plants and at different developmental stages, but its transcripts were most abundant in reproductive organs. Compared with that of the male-fertile line, the level of GhSERK1 mRNA was lower in the anther of the male-sterile cotton line, in which the pollen development was defected. Taken together, these findings illustrated that the GhSERK1 play a critical role during the anther formation, and may also have a broad role in other aspects of plant development.

关键词: cotton (Gossypium hirsutum), somatic embryogenesis receptor-like kinase (SERK), reproductive organs, anther development

Abstract: A novel gene, GhSERK1, was identified in cotton. It encoded a protein belonging to the somatic embryogenesis receptorlike kinase (SERK) family. The genomic sequence of GhSERK1 was 6 920 bp in length, containing a predicted transcriptional start site (TSS). Its full-length cDNA was 2 502 bp, encoding a protein of 627 amino acids. Sequence analysis of GhSERK1 revealed high levels of similarity to other reported SERKs, as well as a conserved intron/exon structure that was unique to members of the SERK family. Expression analysis showed that GhSERK1 mRNA was present in all organs of cotton plants and at different developmental stages, but its transcripts were most abundant in reproductive organs. Compared with that of the male-fertile line, the level of GhSERK1 mRNA was lower in the anther of the male-sterile cotton line, in which the pollen development was defected. Taken together, these findings illustrated that the GhSERK1 play a critical role during the anther formation, and may also have a broad role in other aspects of plant development.

Key words: cotton (Gossypium hirsutum), somatic embryogenesis receptor-like kinase (SERK), reproductive organs, anther development

SHI Ya-li, WU Xiao-ping, MENG Zhi-gang, GUO San-dui. Cloning and Characterization of a Somatic Embryogenesis Receptor-Like Kinase Gene in Cotton (Gossypium hirsutum)[J]. Journal of Integrative Agriculture, 2012, 12(6): 898-909.

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Cloning and Characterization of a Somatic Embryogenesis Receptor-Like Kinase Gene in Cotton (Gossypium hirsutum)

Cloning and Characterization of a Somatic Embryogenesis Receptor-Like Kinase Gene in Cotton (Gossypium hirsutum)

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