Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (6): 1087-1097.DOI: 10.1016/S2095-3119(13)60328-7

• 论文 • 上一篇    下一篇

Microbial Community Dynamics During Biogas Slurry and Cow Manure Compost

 ZHAO Hong-yan, LI Jie, LIU Jing-jing, LÜ Yu-cai, WANG Xiao-fen , CUI Zong-jun   

  1. 1 College of Agronomy and Biotechnology/Center of Biomass Engineering, China Agricultural University, Beijing 100193, P.R.China
    2 Agronomy of Yanbian University, Yanji 133002, P.R.China
    3 Alan G. MacDiarmid Research Institute of Renewable Energy, China Three Gorges University, Yichang 443002, P.R.China
  • 收稿日期:2012-09-18 出版日期:2013-06-01 发布日期:2013-06-09
  • 通讯作者: Correspondence CUI Zong-jun, Tel/Fax: +86-10-62731857, E-mail: acuizj@cau.edu.cn
  • 作者简介:ZHAO Hong-yan, Tel/Fax: +86-10-62733437, E-mail: zhy@ybu.edu.cn
  • 基金资助:

    This work was supported by the National 863 Program of China (2012AA101803) and the National Key Technology R&D Program of China (2012BAD14B06, 2012BAD14B01).

Microbial Community Dynamics During Biogas Slurry and Cow Manure Compost

 ZHAO Hong-yan, LI Jie, LIU Jing-jing, LÜ Yu-cai, WANG Xiao-fen , CUI Zong-jun   

  1. 1 College of Agronomy and Biotechnology/Center of Biomass Engineering, China Agricultural University, Beijing 100193, P.R.China
    2 Agronomy of Yanbian University, Yanji 133002, P.R.China
    3 Alan G. MacDiarmid Research Institute of Renewable Energy, China Three Gorges University, Yichang 443002, P.R.China
  • Received:2012-09-18 Online:2013-06-01 Published:2013-06-09
  • Contact: Correspondence CUI Zong-jun, Tel/Fax: +86-10-62731857, E-mail: acuizj@cau.edu.cn
  • About author:ZHAO Hong-yan, Tel/Fax: +86-10-62733437, E-mail: zhy@ybu.edu.cn
  • Supported by:

    This work was supported by the National 863 Program of China (2012AA101803) and the National Key Technology R&D Program of China (2012BAD14B06, 2012BAD14B01).

PDF

摘要： This study evaluated the microbial community dynamics and maturation time of two compost systems: biogas slurry compost and cow manure compost, with the aim of evaluating the potential utility of a biogas slurry compost system. Denaturing gradient gel electrophoresis (DGGE), gene clone library, temperature, C/N ratio, and the germination index were employed for the investigation, cow manure compost was used as the control. Results showed that the basic strip and dominant strips of the DGGE bands for biogas slurry compost were similar to those of cow manure compost, but the brightness of the respective strips for each system were different. Shannon-Weaver indices of the two compost systems differed, possessing only 22% similarity in the primary and maturity stages of the compost process. Using bacterial 16S rRNA gene clone library analysis, 88 bacterial clones were detected. Further, 18 and 13 operational taxonomic units (OTUs) were present in biogas slurry and cow manure compost, respectively. The 18 OTUs of the biogas slurry compost belonged to nine bacterial genera, of which the dominant strains were Bacillus sp. and Carnobacterium sp.; the 13 OTUs of the cow manure compost belonged to eight bacterial genera, of which the dominant strains were Psychrobacter sp., Pseudomonas sp., and Clostridium sp. Results demonstrated that the duration of the thermophilic phase (more than 50°C) for biogas slurry compost was 8 d less than the according duration for cow manure compost, and the maturation times for biogas slurry and cow manure compost were 45 and 60 d, respectively. It is an effective biogas slurry assimilate technology by application of biogas slurry as nitrogen additives in the manufacture of organic fertilizer.

关键词: biogas slurry fermentation compost , denaturing gradient gel electrophoresis (DGGE) , gene clone library , maturity

Abstract: This study evaluated the microbial community dynamics and maturation time of two compost systems: biogas slurry compost and cow manure compost, with the aim of evaluating the potential utility of a biogas slurry compost system. Denaturing gradient gel electrophoresis (DGGE), gene clone library, temperature, C/N ratio, and the germination index were employed for the investigation, cow manure compost was used as the control. Results showed that the basic strip and dominant strips of the DGGE bands for biogas slurry compost were similar to those of cow manure compost, but the brightness of the respective strips for each system were different. Shannon-Weaver indices of the two compost systems differed, possessing only 22% similarity in the primary and maturity stages of the compost process. Using bacterial 16S rRNA gene clone library analysis, 88 bacterial clones were detected. Further, 18 and 13 operational taxonomic units (OTUs) were present in biogas slurry and cow manure compost, respectively. The 18 OTUs of the biogas slurry compost belonged to nine bacterial genera, of which the dominant strains were Bacillus sp. and Carnobacterium sp.; the 13 OTUs of the cow manure compost belonged to eight bacterial genera, of which the dominant strains were Psychrobacter sp., Pseudomonas sp., and Clostridium sp. Results demonstrated that the duration of the thermophilic phase (more than 50°C) for biogas slurry compost was 8 d less than the according duration for cow manure compost, and the maturation times for biogas slurry and cow manure compost were 45 and 60 d, respectively. It is an effective biogas slurry assimilate technology by application of biogas slurry as nitrogen additives in the manufacture of organic fertilizer.

Key words: biogas slurry fermentation compost , denaturing gradient gel electrophoresis (DGGE) , gene clone library , maturity