Development of SRAP Markers Linked to a Gene for Stem Nematode Resistance in Sweetpotato, Ipomoea batatas (L.) Lam.

doi:10.1016/S2095-3119(13)60241-5

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (3): 414-419.DOI: 10.1016/S2095-3119(13)60241-5

Development of SRAP Markers Linked to a Gene for Stem Nematode Resistance in Sweetpotato, Ipomoea batatas (L.) Lam.

ZHAO Ning, ZHAI Hong, YU Xiao-xia, LIU Zhe-sheng, HE Shao-zhen, LI Qiang, MA Dai-fu , LIU Qing-chang

1.Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization, Ministry of Education/China Agricultural University, Beijing 100193, P.R.China
2.Xuzhou Sweetpotato Research Center, Xuzhou 221121, P.R.China

收稿日期:2011-08-16 出版日期:2013-03-01 发布日期:2013-03-10
通讯作者: Correspondence LIU Qing-chang, Tel/Fax: +86-10-62733710, E-mail: liuqc@cau.edu.cn
基金资助:
This work was supported by China Agriculture Research System (CARS-11, Sweetpotato) and the National 863 Program of China (2012AA101204).

Development of SRAP Markers Linked to a Gene for Stem Nematode Resistance in Sweetpotato, Ipomoea batatas (L.) Lam.

ZHAO Ning, ZHAI Hong, YU Xiao-xia, LIU Zhe-sheng, HE Shao-zhen, LI Qiang, MA Dai-fu , LIU Qing-chang

1.Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization, Ministry of Education/China Agricultural University, Beijing 100193, P.R.China
2.Xuzhou Sweetpotato Research Center, Xuzhou 221121, P.R.China

Received:2011-08-16 Online:2013-03-01 Published:2013-03-10
Contact: Correspondence LIU Qing-chang, Tel/Fax: +86-10-62733710, E-mail: liuqc@cau.edu.cn
Supported by:
This work was supported by China Agriculture Research System (CARS-11, Sweetpotato) and the National 863 Program of China (2012AA101204).

摘要/Abstract

摘要： Sequence-related amplification polymorphism (SRAP) markers closely linked to stem nematode resistance gene were developed in sweetpotato, Ipomoea batatas (L.) Lam. Using bulked segregant analysis (BSA), 200 SRAP primer combinations were screened with the resistant and susceptible bulked DNA from the 196 progenies of an F1 single-cross population of resistant parent Xu 781×susceptible parent Xushu 18, 77 of them showed polymorphic bands between resistant and susceptible DNA. Primer combinations detecting polymorphism between the two bulks were used to screen both parents and 10 individuals from each of the bulks. The results showed that primer combination A9B4 produced 3 specific bands in the resistant plants but not in the susceptible plants, suggesting that the markers, named Nsp1, Nsp2 and Nsp3, respectively, linked to a gene for stem nematode resistance. Primer combination A3B6 also produced a SRAP marker named Nsp4 linking to the resistance gene. Amplified analysis of the 196 F1 individuals indicated that the genetic distance between these markers and the resistance gene was 4.7, 4.7, 6.3, and 9.6 cM, respectively.

关键词: Ipomoea batatas (L.) Lam. , SRAP marker , stem nematode

Abstract: Sequence-related amplification polymorphism (SRAP) markers closely linked to stem nematode resistance gene were developed in sweetpotato, Ipomoea batatas (L.) Lam. Using bulked segregant analysis (BSA), 200 SRAP primer combinations were screened with the resistant and susceptible bulked DNA from the 196 progenies of an F1 single-cross population of resistant parent Xu 781×susceptible parent Xushu 18, 77 of them showed polymorphic bands between resistant and susceptible DNA. Primer combinations detecting polymorphism between the two bulks were used to screen both parents and 10 individuals from each of the bulks. The results showed that primer combination A9B4 produced 3 specific bands in the resistant plants but not in the susceptible plants, suggesting that the markers, named Nsp1, Nsp2 and Nsp3, respectively, linked to a gene for stem nematode resistance. Primer combination A3B6 also produced a SRAP marker named Nsp4 linking to the resistance gene. Amplified analysis of the 196 F1 individuals indicated that the genetic distance between these markers and the resistance gene was 4.7, 4.7, 6.3, and 9.6 cM, respectively.

Key words: Ipomoea batatas (L.) Lam. , SRAP marker , stem nematode

ZHAO Ning, ZHAI Hong, YU Xiao-xia, LIU Zhe-sheng, HE Shao-zhen, LI Qiang, MA Dai-fu , LIU Qing-chang. Development of SRAP Markers Linked to a Gene for Stem Nematode Resistance in Sweetpotato, Ipomoea batatas (L.) Lam.[J]. Journal of Integrative Agriculture, 2013, 12(3): 414-419.

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Development of SRAP Markers Linked to a Gene for Stem Nematode Resistance in Sweetpotato, Ipomoea batatas (L.) Lam.

Development of SRAP Markers Linked to a Gene for Stem Nematode Resistance in Sweetpotato, Ipomoea batatas (L.) Lam.

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