Resistance to the Whitefly Aleurotrachelus socialis (Hemiptera: Aleyrodidae) and SSR Marker Identifi cation in Advanced Populations of the Hybrid Manihot esculenta subsp. Manihot fl abellifolia

doi:10.1016/S2095-3119(13)60661-9

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (12): 2217-2228.DOI: 10.1016/S2095-3119(13)60661-9

Resistance to the Whitefly Aleurotrachelus socialis (Hemiptera: Aleyrodidae) and SSR Marker Identifi cation in Advanced Populations of the Hybrid Manihot esculenta subsp. Manihot fl abellifolia

Arturo Carabalí, James Montoya-Lerma, Anthony C Belloti, Martin Fregene , Gerardo

1.Deparment of Biology, Universidad del Valle, AA 25360, Cali-Valle, Colombia
2.International Center of Tropical Agriculture (CIAT), AA 6713, Cali-Valle, Colombia

收稿日期:2012-11-19 出版日期:2013-12-01 发布日期:2013-12-17
通讯作者: Arturo Carabali, Tel: +57-23-212100, E-mail: arturo.carabali@gmail.com
基金资助:
To reviewers and editors, who, with their comments and suggestions, improved the paper. This paper is dedicated to the memory of Dr. A Bellotti, a great scientist, mentor and a trusted friend.

Resistance to the Whitefly Aleurotrachelus socialis (Hemiptera: Aleyrodidae) and SSR Marker Identifi cation in Advanced Populations of the Hybrid Manihot esculenta subsp. Manihot fl abellifolia

Arturo Carabalí, James Montoya-Lerma, Anthony C Belloti, Martin Fregene , Gerardo

1.Deparment of Biology, Universidad del Valle, AA 25360, Cali-Valle, Colombia
2.International Center of Tropical Agriculture (CIAT), AA 6713, Cali-Valle, Colombia

Received:2012-11-19 Online:2013-12-01 Published:2013-12-17
Contact: Arturo Carabali, Tel: +57-23-212100, E-mail: arturo.carabali@gmail.com
Supported by:
To reviewers and editors, who, with their comments and suggestions, improved the paper. This paper is dedicated to the memory of Dr. A Bellotti, a great scientist, mentor and a trusted friend.

摘要/Abstract

摘要： Genes resistant to Aleurotrachelus socialis were transferred to the F1 from the interspecifi c hybrid wild species of Manihot fl abellifolia to M. esculenta and two advanced generations of backcrosses (BC1 and BC2). We characterized the resistance of A. socialis transferred to BC2 parents (CW67-160, CW67-130, CW67-44), MTAI-8 (BC1), resistant (CMB9B-73) and susceptible (CMB9B-104) genotypes from contrasting pools, and resistant (MEcu-72) and susceptible (CMC-40) genotypes. Whitefl y demography and biology were evaluated. SSR molecular markers associated with a phenotypic response of plant resistance were detected in segregating populations (BC2). Results showed that although female survival time was similar on all hosts, the lowest averages of longevity, fecundity and oviposition rate were observed in the resistant control MEcu- 72, only being signifi cantly similar to the parent CW67-130. When the BC1 and BC2 populations were compared, it was found that A. socialis fecundity was eight times lower on CMB9B-73 progeny than on CW67-130, expressing the highest levels of resistance to the whitefl y. Ten genotypes of CMB9A and CMB9B family had the best segregation. A total of 486 microsatellite primers were evaluated using bulked segregant analysis (BSA), 11 showed polymorphism between the contrasting pools and only one showed signifi cant differences between resistant and susceptible individuals. In conclusion, fecundity was the parameter that impacted most on the intrinsic rate of A. socialis population growth.

关键词: cassava , wild parents , interspecifi c hybrids , whitefl y resistance , BSA

Abstract: Genes resistant to Aleurotrachelus socialis were transferred to the F1 from the interspecifi c hybrid wild species of Manihot fl abellifolia to M. esculenta and two advanced generations of backcrosses (BC1 and BC2). We characterized the resistance of A. socialis transferred to BC2 parents (CW67-160, CW67-130, CW67-44), MTAI-8 (BC1), resistant (CMB9B-73) and susceptible (CMB9B-104) genotypes from contrasting pools, and resistant (MEcu-72) and susceptible (CMC-40) genotypes. Whitefl y demography and biology were evaluated. SSR molecular markers associated with a phenotypic response of plant resistance were detected in segregating populations (BC2). Results showed that although female survival time was similar on all hosts, the lowest averages of longevity, fecundity and oviposition rate were observed in the resistant control MEcu- 72, only being signifi cantly similar to the parent CW67-130. When the BC1 and BC2 populations were compared, it was found that A. socialis fecundity was eight times lower on CMB9B-73 progeny than on CW67-130, expressing the highest levels of resistance to the whitefl y. Ten genotypes of CMB9A and CMB9B family had the best segregation. A total of 486 microsatellite primers were evaluated using bulked segregant analysis (BSA), 11 showed polymorphism between the contrasting pools and only one showed signifi cant differences between resistant and susceptible individuals. In conclusion, fecundity was the parameter that impacted most on the intrinsic rate of A. socialis population growth.

Key words: cassava , wild parents , interspecifi c hybrids , whitefl y resistance , BSA

Arturo Carabalí, James Montoya-Lerma, Anthony C Belloti, Martin Fregene , Gerardo. Resistance to the Whitefly Aleurotrachelus socialis (Hemiptera: Aleyrodidae) and SSR Marker Identifi cation in Advanced Populations of the Hybrid Manihot esculenta subsp. Manihot fl abellifolia[J]. Journal of Integrative Agriculture, 2013, 12(12): 2217-2228.

Arturo Carabalí, James Montoya-Lerma, Anthony C Belloti, Martin Fregene , Gerardo . Resistance to the Whitefly Aleurotrachelus socialis (Hemiptera: Aleyrodidae) and SSR Marker Identifi cation in Advanced Populations of the Hybrid Manihot esculenta subsp. Manihot fl abellifolia[J]. Journal of Integrative Agriculture, 2013, 12(12): 2217-2228.

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Resistance to the Whitefly Aleurotrachelus socialis (Hemiptera: Aleyrodidae) and SSR Marker Identifi cation in Advanced Populations of the Hybrid Manihot esculenta subsp. Manihot fl abellifolia

Resistance to the Whitefly Aleurotrachelus socialis (Hemiptera: Aleyrodidae) and SSR Marker Identifi cation in Advanced Populations of the Hybrid Manihot esculenta subsp. Manihot fl abellifolia

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