Identification and Molecular Tagging of Leaf Rust Resistance Gene (Lr24) in Wheat

doi:10.1016/S1671-2927(11)60190-9

Journal of Integrative Agriculture ›› 2011, Vol. 10 ›› Issue (12): 1898-1905.DOI: 10.1016/S1671-2927(11)60190-9

Identification and Molecular Tagging of Leaf Rust Resistance Gene (Lr24) in Wheat

ZHANG Na, YANG Wen-xiang , LIU Da-qun

1.Department of Plant Pathology/Biological Control Center of Plant Diseases and Plant Pests of Hebei Province, Agricultural University of Hebei, Baoding 071001, P.R.China

收稿日期:2011-01-25 出版日期:2011-12-01 发布日期:2011-12-19
通讯作者: YANG Wen-xiang, Tel: +86-312-7528585, Fax: +86-312-7528500, E-mail: wenxiangyang2003@163.com;LIU Da-qun, Tel: +86-312-7528500, Fax: +86-312-7528500, E-mail: ldq@hebau.edu.cn
作者简介:ZHANG Na, E-mail: zn0318@126.com
基金资助:
The authors thank the National Natural Science Foundation of China (30771391), the transgenic Major Projects, China (2009ZX08002-012B) and the Specialized Research Fund for the Doctoral Program of Higher Education of China (20101302120005) for funding the research project.

Identification and Molecular Tagging of Leaf Rust Resistance Gene (Lr24) in Wheat

ZHANG Na, YANG Wen-xiang , LIU Da-qun

1.Department of Plant Pathology/Biological Control Center of Plant Diseases and Plant Pests of Hebei Province, Agricultural University of Hebei, Baoding 071001, P.R.China

Received:2011-01-25 Online:2011-12-01 Published:2011-12-19
Contact: YANG Wen-xiang, Tel: +86-312-7528585, Fax: +86-312-7528500, E-mail: wenxiangyang2003@163.com;LIU Da-qun, Tel: +86-312-7528500, Fax: +86-312-7528500, E-mail: ldq@hebau.edu.cn
About author:ZHANG Na, E-mail: zn0318@126.com
Supported by:
The authors thank the National Natural Science Foundation of China (30771391), the transgenic Major Projects, China (2009ZX08002-012B) and the Specialized Research Fund for the Doctoral Program of Higher Education of China (20101302120005) for funding the research project.

摘要/Abstract

摘要： This research was aimed to develop AFLP markers co-segregated with gene Lr24 and validate the using for marker assisted selection (MAS). An F2 population developed from the cross between the resistant line TcLr24 and the susceptible line Thatcher was tested for resistance to the Puccinia triticina races BGQQ and SHRT using for genetic analysis and molecular marker. A total of 224 AFLP primer combinations were used to test the resistant and susceptible parents, as well as the resistant bulk and the susceptible bulk. Four AFLP markers, P-AGA/M-CTT289 bp, P-AGC/M-CAC188 bp, P-AGC/MCAC 162 bp, and P-ACG/M-CGC239 bp, were co-segregated with Lr24. The AFLP fragment from the primer combination PACG/ M-CGC was cloned, sequenced and converted into a STS marker named as ASTS212. Thatcher backgrounded NILs and 115 varieties were examined by using this STS marker and the marker SCS1302607 developed by Gupta. 5R615, 5R616, 1R13, and 1R17 were identified and validated to contain gene Lr24. The marker is dominant and may be useful in identification the resistance gene Lr24 in wheat and wheat breeding programs.

关键词: wheat leaf rust, Lr24 gene, AFLP, STS, MAS

Abstract: This research was aimed to develop AFLP markers co-segregated with gene Lr24 and validate the using for marker assisted selection (MAS). An F2 population developed from the cross between the resistant line TcLr24 and the susceptible line Thatcher was tested for resistance to the Puccinia triticina races BGQQ and SHRT using for genetic analysis and molecular marker. A total of 224 AFLP primer combinations were used to test the resistant and susceptible parents, as well as the resistant bulk and the susceptible bulk. Four AFLP markers, P-AGA/M-CTT289 bp, P-AGC/M-CAC188 bp, P-AGC/MCAC 162 bp, and P-ACG/M-CGC239 bp, were co-segregated with Lr24. The AFLP fragment from the primer combination PACG/ M-CGC was cloned, sequenced and converted into a STS marker named as ASTS212. Thatcher backgrounded NILs and 115 varieties were examined by using this STS marker and the marker SCS1302607 developed by Gupta. 5R615, 5R616, 1R13, and 1R17 were identified and validated to contain gene Lr24. The marker is dominant and may be useful in identification the resistance gene Lr24 in wheat and wheat breeding programs.

Key words: wheat leaf rust, Lr24 gene, AFLP, STS, MAS

ZHANG Na, YANG Wen-xiang , LIU Da-qun. Identification and Molecular Tagging of Leaf Rust Resistance Gene (Lr24) in Wheat[J]. Journal of Integrative Agriculture, 2011, 10(12): 1898-1905.

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Identification and Molecular Tagging of Leaf Rust Resistance Gene (Lr24) in Wheat

Identification and Molecular Tagging of Leaf Rust Resistance Gene (Lr24) in Wheat

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