DNA Polymorphisms of 5´-Flanking Region of Insulin-Like Growth Factor 1 Gene and Their Association with Reproduction Traits in Goats

doi:10.1016/S1671-2927(11)60158-2

Journal of Integrative Agriculture ›› 2011, Vol. 10 ›› Issue (10): 1609-1617.DOI: 10.1016/S1671-2927(11)60158-2

DNA Polymorphisms of 5´-Flanking Region of Insulin-Like Growth Factor 1 Gene and Their Association with Reproduction Traits in Goats

WANG Ping-qing, TAN Ying, ZHANG Bao-yun, CHU Ming-xing, DENG La-mei, FAN Qi , LIUChong-xu

1.College of Bioengineering, Chongqing University
2.Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation, Ministry of Agriculture/Institute of Animal Science,Chinese Academy of Agricultural Sciences

收稿日期:2010-08-03 出版日期:2011-10-01 发布日期:2011-10-18
通讯作者: Correspondence WANG Ping-qing, Professor, Tel: +86-23-65112753, E-mail: wang_pq@21cn.com
作者简介:WANG Ping-qing, Professor, Tel: +86-23-65112753, E-mail: wang_pq@21cn.com
基金资助:
This work was supported by the earmarked fund for Modern Agro-Industry Technology Research System of China (nycytx-39) and the Natural Science Foundation Project of CQ CSTC, Chongqing City, China (CSTC, 2009BA1066).

DNA Polymorphisms of 5´-Flanking Region of Insulin-Like Growth Factor 1 Gene and Their Association with Reproduction Traits in Goats

WANG Ping-qing, TAN Ying, ZHANG Bao-yun, CHU Ming-xing, DENG La-mei, FAN Qi , LIUChong-xu

1.College of Bioengineering, Chongqing University
2.Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation, Ministry of Agriculture/Institute of Animal Science,Chinese Academy of Agricultural Sciences

Received:2010-08-03 Online:2011-10-01 Published:2011-10-18
Contact: Correspondence WANG Ping-qing, Professor, Tel: +86-23-65112753, E-mail: wang_pq@21cn.com
About author:WANG Ping-qing, Professor, Tel: +86-23-65112753, E-mail: wang_pq@21cn.com
Supported by:
This work was supported by the earmarked fund for Modern Agro-Industry Technology Research System of China (nycytx-39) and the Natural Science Foundation Project of CQ CSTC, Chongqing City, China (CSTC, 2009BA1066).

摘要/Abstract

摘要： Research on the identity of genes and their relationship with traits of economic importance in farm animals could assist in the selection of livestock. In this study, the polymorphisms of insulin-like growth factor 1 (IGF1) gene in 561 goats of ten breeds were detected by polymerase chain reaction (PCR)-single strand conformation polymorphism (SSCP) and their association with litter size and birth weight in three breeds were investigated. The effects of IGF1 polymorphisms on the breeding value for litter size and birth weight were examined using least square methods. Two deletions (CA) were detected in the microsatellite and two mutations (A1637G, T1640C) were found in 5´-flanking regulatory region. No significant association between the polymorphisms in 5´-flanking region of IGF1 and birth weight was found in the three breeds of goats. In Gulin Ma goats, two polymorphisms were found to affect litter size traits. In Chuandong White goats and Guizhou White goats, no significant difference (P<0.05) in litter size between goats carrying different genotypes was observed. Further evaluation and confirmation studies in more goat populations with larger sample sizes are necessary.

关键词:

IGF1 gene, DNA polymorphism, litter size, birth weight, goat

Abstract: Research on the identity of genes and their relationship with traits of economic importance in farm animals could assist in the selection of livestock. In this study, the polymorphisms of insulin-like growth factor 1 (IGF1) gene in 561 goats of ten breeds were detected by polymerase chain reaction (PCR)-single strand conformation polymorphism (SSCP) and their association with litter size and birth weight in three breeds were investigated. The effects of IGF1 polymorphisms on the breeding value for litter size and birth weight were examined using least square methods. Two deletions (CA) were detected in the microsatellite and two mutations (A1637G, T1640C) were found in 5´-flanking regulatory region. No significant association between the polymorphisms in 5´-flanking region of IGF1 and birth weight was found in the three breeds of goats. In Gulin Ma goats, two polymorphisms were found to affect litter size traits. In Chuandong White goats and Guizhou White goats, no significant difference (P<0.05) in litter size between goats carrying different genotypes was observed. Further evaluation and confirmation studies in more goat populations with larger sample sizes are necessary.

Key words:

IGF1 gene, DNA polymorphism, litter size, birth weight, goat

WANG Ping-qing, TAN Ying, ZHANG Bao-yun, CHU Ming-xing, DENG La-mei, FAN Qi , LIUChong-xu. DNA Polymorphisms of 5´-Flanking Region of Insulin-Like Growth Factor 1 Gene and Their Association with Reproduction Traits in Goats [J]. Journal of Integrative Agriculture, 2011, 10(10): 1609-1617.

WANG Ping-qing, TAN Ying, ZHANG Bao-yun, CHU Ming-xing, DENG La-mei, FAN Qi , LIUChong-xu . DNA Polymorphisms of 5´-Flanking Region of Insulin-Like Growth Factor 1 Gene and Their Association with Reproduction Traits in Goats [J]. Journal of Integrative Agriculture, 2011, 10(10): 1609-1617.

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DNA Polymorphisms of 5´-Flanking Region of Insulin-Like Growth Factor 1 Gene and Their Association with Reproduction Traits in Goats

DNA Polymorphisms of 5´-Flanking Region of Insulin-Like Growth Factor 1 Gene and Their Association with Reproduction Traits in Goats

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