柑橘黄龙病菌效应蛋白通过靶向甜橙中 ARM 重复蛋白来抑制水杨酸介导的宿主免疫反应

doi:10.1016/j.jia.2025.11.024

Journal of Integrative Agriculture ›› 2026, Vol. 25 ›› Issue (10): 4248-4256.DOI: 10.1016/j.jia.2025.11.024

柑橘黄龙病菌效应蛋白通过靶向甜橙中 ARM 重复蛋白来抑制水杨酸介导的宿主免疫反应

收稿日期:2025-05-23 修回日期:2025-11-15 接受日期:2025-06-18 出版日期:2026-10-20 发布日期:2026-09-21

An effector from ‘Candidatus Liberibacter asiaticus’ targets the armadillo (ARM)-repeated protein of Citrus sinensis to inhibit salicylic acid-mediated host immunity

Yalin Mei^1,²^*, Zaiyu Yang^1,²^*, Shushe Zhang^1,², Pan Shen^1,³, Changyong Zhou^1,², Xuefeng Wang^1,²^#

¹Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Citrus Research Institute, Southwest University, Chongqing 400712, China
² National Citrus Engineering Research Center, Citrus Research Institute, Southwest University, Chongqing 400712, China
³College of food and Biology, Jingchu University of Technology, Jingmen 448000, China

Received:2025-05-23 Revised:2025-11-15 Accepted:2025-06-18 Online:2026-10-20 Published:2026-09-21
About author:#Correspondence Xuefeng Wang, E-mail: wangxuefeng@cric.cn
Supported by:
This work was supported by the National Natural Sciences Foundation of China (U23A20196), the National Key Research and Development Program of China (2021YFD1400800), the Special Fund for Youth Team of Southwest University, China (SWU-XJLJ202310), the Innovation Research 2035 Pilot Plan of Southwest University, China (SWU-5331000008), and the Southwest University Research and Innovation Project, China (SWUB 25096 and SWUS24208).

摘要/Abstract

摘要：

柑橘黄龙病（HLB）已对全球柑橘产业造成了广泛损害。黄龙病传播范围广，尚无有效药物根治黄龙病，黄龙病的主要病原体“亚洲韧皮部杆菌”（CLas）利用效应子来调节宿主的防御反应，但这些效应子的作用机制仍不清楚。本研究前期发现，柑橘转基因CLas0185能够促进HLB侵染，酵母双杂筛库发现潜在互作蛋白CsARM26, 并进一步利用利用酵母双杂 (Y2H)、烟草双分子荧光（BiFC）以及免疫共沉淀（Co-IP）技术证明，柑橘重复蛋白 CsARM26 在体内和体外均与 CLIBASIA_00185（CLas0185）相互作用。为探究两者互作分子机制，在本氏烟草中瞬时共表达CLas0185与CsARM26，利用蛋蛋白免疫印迹（WB）技术检测发现CLas0185 增加了 CsARM26 的丰度，而 CsARM26 能促进CLas蛋白降解。进一步发现，CsAMR26并不具有E3连接酶活性，表明CsARM26可能通过与相关E3连接酶互作促进CLas0185降解。此外，CLas0185 和 CsARM26 的瞬时共表达促进了柑橘黄溃疡病的侵染。转基因 CsARM26 柑橘植株抑制了游离水杨酸（SA）的积累以及与 SA 相关基因的表达，CsARM26转录组数据表明免疫相关基因显著下调，以及水杨酸相关通路受到显著影响。本研究结果表明，CsARM26蛋白参与了柑橘对 CLas 互作的免疫反应，为 CLas 感染的分子机制进一步研究提供了基础。

Abstract: Citrus Huanglongbing (HLB) has caused extensive damage to the global citrus industry. ‘Candidatus Liberibacter asiaticus’ (CLas), the primary causal agent of HLB, utilizes effectors to modulate host defense responses, though the mechanisms of these effectors remain unclear. This study demonstrates that the citrus armadillo (ARM)-repeated protein CsARM26 interacted with CLIBASIA_00185 (CLas0185) in vivo and in vitro. CLas0185 enhanced the abundance of CsARM26, whereas CsARM26 destabilized the effector. Additionally, the transient co-expression of CLas0185 and CsARM26 facilitated infection by Xanthomonas citri subsp. citri. Moreover, transgenic CsARM26 citrus plants suppressed the accumulation of free salicylic acid (SA) and the expression of SA-associated genes. This study reveals that an ARM-repeated protein plays a role in the immune response to the CLas–citrus interaction, establishing a foundation for further investigation of the molecular mechanisms of CLas infection.

Key words: Huanglongbing , effector , SA , armadillo , CsARM26

Yalin Mei, Zaiyu Yang, Shushe Zhang, Pan Shen, Changyong Zhou, Xuefeng Wang. 柑橘黄龙病菌效应蛋白通过靶向甜橙中 ARM 重复蛋白来抑制水杨酸介导的宿主免疫反应[J]. Journal of Integrative Agriculture, 2026, 25(10): 4248-4256.

Yalin Mei, Zaiyu Yang, Shushe Zhang, Pan Shen, Changyong Zhou, Xuefeng Wang. An effector from ‘Candidatus Liberibacter asiaticus’ targets the armadillo (ARM)-repeated protein of Citrus sinensis to inhibit salicylic acid-mediated host immunity[J]. Journal of Integrative Agriculture, 2026, 25(10): 4248-4256.

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柑橘黄龙病菌效应蛋白通过靶向甜橙中 ARM 重复蛋白来抑制水杨酸介导的宿主免疫反应

An effector from ‘Candidatus Liberibacter asiaticus’ targets the armadillo (ARM)-repeated protein of Citrus sinensis to inhibit salicylic acid-mediated host immunity

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