Journal of Integrative Agriculture ›› 2026, Vol. 25 ›› Issue (3): 989-1008.DOI: 10.1016/j.jia.2025.09.005

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VaMYB4a通过双重调控CBF-COR和ABA信号通路增强葡萄耐寒性

  

  • 收稿日期:2024-12-04 修回日期:2025-09-12 接受日期:2025-04-29 出版日期:2026-03-20 发布日期:2026-02-06

Amur grape VaMYB4a mediates grapevine cold tolerance via dual regulation of CBF–COR and ABA pathways

Qinhan Yu1*, Yue Sun2*, Yaping Xie2, Jiaxin Li2, Rong Wang2, Qiaoling Zheng1, Chang Liu1, Ningbo Zhang2, 3, 4, 5, 6, Weirong Xu1, 2, 3, 4, 5, 6#   

  1. 1 School of Life Sciences, Ningxia University, Yinchuan 750021, China

    2 College of Enology and Horticulture, Ningxia University, Yinchuan 750021, China

    3 Engineering Research Center of Grape and Wine, Ministry of Education, Ningxia University, Yinchuan 750021, China

    4 Ningxia Grape and Wine Research Institute, Ningxia University, Yinchuan 750021, China

    5 Key Laboratory of Modern Molecular Breeding for Dominant and Special Crops in Ningxia, Yinchuan 750021, China

    6 State Key Laboratory of Efficient Production of Forest Resources, Yinchuan 750021, China

  • Received:2024-12-04 Revised:2025-09-12 Accepted:2025-04-29 Online:2026-03-20 Published:2026-02-06
  • About author:#Correspondence Weirong Xu, E-mail: xuwr@nxu.edu.cn * These authors contributed equally to this study.
  • Supported by:

    This work was supported by the Ningxia Hui Autonomous Region Key R&D Program, China (2023BCF01003), the National Natural Science Foundation of China (32472711 and 32060672) and the Agricultural Breeding Project of Ningxia Hui Autonomous Region, China (NXNYYZ202101).

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摘要：

低温胁迫是限制作物产量的关键因素，尤其在温带气候条件下尤为突出。尽管脱落酸在寒冷胁迫响应中的作用已被广泛认可，但转录因子如何介导ABA依赖的耐寒机制仍不明确。本研究从山葡萄（Vitis amurensis Rupr.）中鉴定出MYB转录因子VaMYB4a，其通过整合ABA信号与CBF-COR通路协同调控低温适应性。通过构建拟南芥、葡萄愈伤组织及欧洲葡萄（Vitis vinifera L.）幼苗中进行过表达和CRISPR/Cas9基因编辑株系，我们发现VaMYB4a通过促进渗透调节、活性氧清除及气孔关闭来增强植株抗冻性。该蛋白以同源二聚体形式发挥作用，其C端结构域对转录激活至关重要。在分子机制上，VaMYB4a可直接上调CBF和COR基因表达，并精细调控ABA信号通路关键因子ABI1和ABF4。值得注意的是，ABA呈现双重调控效应：在短期胁迫下增强VaMYB4a介导的抗冻性，而在长期冷暴露中削弱其功能，揭示低温与激素通路间复杂的级联调控网络。本研究不仅深化了对植物冷适应机制的认知，更为培育气候韧性葡萄品种提供了关键遗传靶标。


Abstract:

Cold stress represents a critical constraint on crop productivity, particularly in temperate climates.  Despite the established role of abscisic acid (ABA) in cold stress responses, the precise mechanisms through which transcription factors mediate ABA-dependent cold tolerance remain elusive.  Here, we identify VaMYB4a, a MYB transcription factor from Vitis amurensis Rupr.  (Amur grape), as a key regulator of cold tolerance.  It integrates ABA signaling with the CBF (C-repeat binding factors)-COR (cold-regulated) pathway to orchestrate cold stress adaptation.  Through a combination of overexpression and CRISPR/Cas9-mediated knockout lines in Arabidopsis thaliana, grape callus, and Vitis vinifera L. seedlings, we demonstrate that VaMYB4a enhances freezing tolerance by promoting osmotic regulation, reactive oxygen species (ROS) scavenging, and stomatal closure.  VaMYB4a functions as a homo-dimer, with its C-terminal domain being essential for transcriptional activation.  Mechanistically, VaMYB4a directly upregulates CBF and COR genes while fine-tuning ABA signaling components such as ABI1 and ABF4.  Notably, ABA exhibits a dual role: enhancing VaMYB4a-mediated freezing tolerance under short-term stress but attenuating its effects during prolonged cold exposure, revealing an intricate regulatory crosstalk between cold and hormonal pathways.  Our work not only advances the molecular understanding of cold adaptation but also provides a promising genetic target for developing stress-resilient grape varieties to mitigate the impacts of climate change.

Key words: Vitis amurensis Rupr. , VaMYB4a , CRISPR/Cas9 , ABA signaling , CBF-COR pathway , cold tolerance