Journal of Integrative Agriculture ›› 2026, Vol. 25 ›› Issue (10): 4002-4013.DOI: 10.1016/j.jia.2024.12.019
四倍体小麦籽粒中独立于主要农艺性状的镉、铜和镁含量位点的遗传鉴定与分析
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收稿日期:2024-09-20修回日期:2024-12-16接受日期:2024-11-04出版日期:2026-10-20发布日期:2026-09-21
Major and stably expressed QTLs for grain cadmium, copper, and magnesium concentrations that are independent of the main agronomic traits in tetraploid wheat
Zhaoyong Zeng1, 2*, Jian Ma1*#, Ying Wang1, Yuxin Lan1, Longxing Su1, Bin Chen1, Huaping Tang1, Deyi Hu2, Bingjie Chen2, Yinggang Xu2, Yang Li2, Xuesong Gao2, Chunji Liu3, Guangdeng Chen2#
1 State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, China
2 College of Resources, Sichuan Agricultural University, Chengdu 611130, China
3 CSIRO Agriculture and Food, St Lucia, QLD 4067, Australia
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Received:2024-09-20Revised:2024-12-16Accepted:2024-11-04Online:2026-10-20Published:2026-09-21 -
About author:#Correspondence Jian Ma, E-mail: jianma@sicau.edu.cn; Guangdeng Chen, Tel: +86-28-86290986, Fax: +86-28-86290983, E-mail: gdchen@sicau.edu.cn * These authors contributed equally to this study. -
Supported by:This study was supported by the National Natural Science Foundation of China (31971944) and the Sichuan Province Science Foundation for Distinguished Young Scholars, China (2022JDJQ0006).
摘要：
籽粒中镉的过量以及铜和镁的缺乏均会对人类健康构成严重威胁。普通小麦育种导致优良种质资源的遗传多样性下降，从而对未来小麦生产产生不利影响。因此，从四倍体小麦中鉴定控制籽粒镉、铜和镁的基因座，并将其导入普通小麦，是实现遗传改良的关键。本研究以四川地方品种四倍体小麦（矮兰麦）和野生二粒小麦（LM001）杂交构建的重组自交系，结合55K单核苷酸多态性（SNP）阵列的连锁图谱和多个环境中的表型数据，鉴定籽粒镉、铜和镁含量的数量性状位点，共鉴定出4个稳定表达的主效位点。其中控制籽粒镉含量的QGrCdc.sau-AM-5A、控制籽粒铜含量的QGrCuc.sau-AM-4A和控制籽粒镁含量的QGrMgc.sau-AM-4A是3个新的主效位点。通过紧密连锁的等位基因特异性PCR（KASP）标记，在不同遗传背景下对这些位点进行了验证。在几个主效位点中，预测到一些具有序列变异的候选基因（TRIDC5AG052690、TRIDC5BG060070、TRIDC4AG008520）可能会影响镉、铜或镁的吸收和转运。相关分析表明，籽粒镉含量与籽粒铜和镁含量之间没有显著相关性，而籽粒铜含量与镁含量则显著相关。此外，籽粒镉、铜和镁含量与11个农艺性状均未表现出相关性。值得注意的是，QGrCuc.sau-AM-4A与QGrMgc.sau-AM-4A共定位，表明它们可能存在共同的生理和/或遗传控制。总的来说，这些稳定表达的位点为进一步的种质改良和精细定位提供了理论依据。
引用本文
Zhaoyong Zeng, Jian Ma, Ying Wang, Yuxin Lan, Longxing Su, Bin Chen, Huaping Tang, Deyi Hu, Bingjie Chen, Yinggang Xu, Yang Li, Xuesong Gao, Chunji Liu, Guangdeng Chen. 四倍体小麦籽粒中独立于主要农艺性状的镉、铜和镁含量位点的遗传鉴定与分析[J]. Journal of Integrative Agriculture, 2026, 25(10): 4002-4013.
Zhaoyong Zeng, Jian Ma, Ying Wang, Yuxin Lan, Longxing Su, Bin Chen, Huaping Tang, Deyi Hu, Bingjie Chen, Yinggang Xu, Yang Li, Xuesong Gao, Chunji Liu, Guangdeng Chen. Major and stably expressed QTLs for grain cadmium, copper, and magnesium concentrations that are independent of the main agronomic traits in tetraploid wheat[J]. Journal of Integrative Agriculture, 2026, 25(10): 4002-4013.
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