Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (6): 2438-2442.DOI: 10.1016/j.jia.2024.12.002

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SiDWARF4编码谷子赤霉素生物合成和形态发生所必需的古巴焦磷酸合成酶（CPS）

  

  • 收稿日期:2024-09-04 修回日期:2024-12-04 接受日期:2024-10-24 出版日期:2025-06-20 发布日期:2025-05-13

SiDWARF4 encodes an ent-copalyl diphosphate synthase (CPS) required for gibberellin biosynthesis and morphogenesis in foxtail millet

Yongchao Li1, Mengmeng Sun1, Rui Zhao1, Jiayi Chen1, Yunhao Chen1, Shuqi Dong1, 2, Xiangyang Yuan1, 2, Xiaorui Li1, 2, Lulu Gao1, Guanghui Yang1, Peiyong Xin3, Shujing Cheng3, Jinfang Chu3, 4, Xiaoqian Chu1#, Jiagang Wang1#   

  1. 1 Houji Laboratory in Shanxi Province/College of Agriculture, Shanxi Agricultural University, Taigu 030801, China

    2 State Key Laboratory of Sustainable Dryland Agriculture (in Preparation), Shanxi Agricultural University, Taigu 030801, China

    3 National Centre for Plant Gene Research (Beijing)/Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China

    4 University of Chinese Academy of Sciences, Beijing 100049, China

  • Received:2024-09-04 Revised:2024-12-04 Accepted:2024-10-24 Online:2025-06-20 Published:2025-05-13
  • About author:#Correspondence Jiagang Wang, E-mail: wjg@sxau.edu.cn; Xiaoqian Chu, E-mail: chuxiaoqian@sxau.edu.cn
  • Supported by:

    This work was supported by the National Natural Science Foundation of China (32200222), the High-level Talents Start-up Fund of Shanxi Agricultural University, China (J242198006), the Start-up Fund of Shanxi Agricultural University, China (2021BQ84), the Shanxi Province Outstanding Doctoral and Post-Doctoral Scholarship Award Foundation, China (SXBYKY2021055 and SXBYKY2022033), and the Houji Laboratory Foundation, China (202204010910001-32).

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摘要：

赤霉素在植物生长发育中起着重要作用。作物矮化主要与赤霉素合成或信号转导途径的突变有关。然而，对作物中与赤霉素合成和调控有关的基因知之甚少。本研究分离到一株极端矮化的自然矮杆突变体dwarf4。基于BSA-seq，证实了由SiDWARF4编码的古巴焦磷酸合成酶（CPS）在谷子GA生物合成过程中催化了牻牛儿基牻牛儿基焦磷酸（GGPP）向古巴焦磷酸（CPP）的转化。在dwarf4中，CPS酶底物GGPP水平升高，导致GAs含量降低。外源GA3处理可以恢复dwarf4的异常表型。SiDWARF4敲除系表型与dwarf4一致。与对照Ci846相比，GGPP含量升高，GAs含量降低，与dwarf4的结果一致。研究结果对改良谷子株型和提高谷子种植密度具有一定的指导意义。