FS2编码一个ARID-HMG转录因子，调控黄瓜果刺密度

doi:10.1016/j.jia.2024.11.024

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (3): 1080-1091.DOI: 10.1016/j.jia.2024.11.024

FS2编码一个ARID-HMG转录因子，调控黄瓜果刺密度

收稿日期:2023-12-07 接受日期:2024-02-27 出版日期:2025-03-20 发布日期:2025-02-28

FS2 encodes an ARID-HMG transcription factor that regulates fruit spine density in cucumber

Hui Du^1*, Yue Chen^1,^2*, Liangrong Xiong¹, Juan Liu¹, Keyan Zhang¹, Ming Pan^1,³, Haifan Wen¹, Huanle He¹, Run Cai¹, Junsong Pan^1,^4#, Gang Wang^1#

¹School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China
²Department of Plant Science and Technology, Shanghai Vocational College of Agriculture and Forestry, Shanghai 201699, China
³Shanghai Agricultural Technology Extension and Service Center, Shanghai 201103, China
⁴Shanghai Collaborative Innovation Center of Agri-Seeds, Shanghai Jiao Tong University, Shanghai 200240, China

Received:2023-12-07 Accepted:2024-02-27 Online:2025-03-20 Published:2025-02-28
About author:Hui Du, E-mail: duhui01@caas.cn; Yue Chen, E-mail: yuechen210@163.com; #Correspondence Junsong Pan, E-mail: jspan71@sjtu.edu.cn; Gang Wang, E-mail: wg770801@sjtu.edu.cn *These authors contributed equally to this study.
Supported by:
This study was supported by the National Natural Science Foundation of China (31972425) and the Shanghai Agriculture Applied Technology Development Program, China (2020-02-08-00-08-F0148).

摘要/Abstract

摘要：

果刺密度是黄瓜重要的商品性状，然而种植面积较大的华北型黄瓜品种大都是密刺表型，这直接影响了黄瓜的外观品质、储藏和运输。在本研究中，我们通过EMS诱变，从华北型密刺品种WD1中鉴定到一个新的少刺突变体（few spines, fs2）。遗传分析发现fs2的少刺表型是由单基因调控的。利用1802株F₂和420株BC₁群体对fs2位点进行精细定位，发现FS2的候选基因Csa4G652850编码一个ARID-HMG转录因子，包含一个A/T-rich interaction domain (ARID)结构域和一个high mobility group box (HMG)结构域。FS2候选基因编码区域的一个SNP (C to T)和一个Indel (40 bp deletion)分别导致fs2突变体中氨基酸变异和翻译提前终止。FS2候选基因在顶芽和幼嫩的子房中高表达。此外，我们发现FS2通过激活Tril基因的表达参与黄瓜果刺起始调节。本研究不仅为阐释黄瓜果刺发育的分子机制提供了重要参考，也为黄瓜果实外观品质育种提供了重要资源。

Abstract:

Fruit spine density is an important commercial trait for cucumber (Cucumis sativus L.). Most North China-type cucumbers that are grown over large areas have a dense-spine phenotype, which directly affects the appearance quality, storage, and transportation of the fruits. Here, we isolated a novel few spines mutant (fs2) from the wild-type (WT) inbred line WD1, a North China-type cucumber with high density fruit spines, by an ethyl methanesulfonate (EMS) mutagenesis treatment. Genetic analysis revealed that the phenotype of fs2 is controlled by a single recessive nuclear gene. We fine-mapped the fs2 locus using F₂ and BC₁ populations (1,802 and 420 individuals, respectively), which showed that the candidate gene of FS2 (Csa4G652850) encodes an ARID-HMG transcription factor containing an AT-rich interaction domain (ARID) and a high mobility group box domain (HMG). One SNP (C to T) and one InDel (a 40-bp deletion) in the coding region of FS2 result in amino acid variation and premature translation termination in the fs2 mutant, respectively. FS2 was found to be highly expressed in the apical buds and young ovaries. In addition, experiments suggest that FS2 participates in the regulation of fruit spine initiation by activating the expression of the Tril gene in cucumber. This work provides not only an important reference for understanding the molecular mechanisms of fruit spine development but also an important resource for fruit appearance quality breeding in cucumber.

Key words: cucumber , few spines , FS2 , trichome , ARID-HMG transcription factor

Hui Du, Yue Chen, Liangrong Xiong, Juan Liu, Keyan Zhang, Ming Pan, Haifan Wen, Huanle He, Run Cai, Junsong Pan, Gang Wang. FS2编码一个ARID-HMG转录因子，调控黄瓜果刺密度[J]. Journal of Integrative Agriculture, 2025, 24(3): 1080-1091.

Hui Du, Yue Chen, Liangrong Xiong, Juan Liu, Keyan Zhang, Ming Pan, Haifan Wen, Huanle He, Run Cai, Junsong Pan, Gang Wang. FS2 encodes an ARID-HMG transcription factor that regulates fruit spine density in cucumber[J]. Journal of Integrative Agriculture, 2025, 24(3): 1080-1091.

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FS2编码一个ARID-HMG转录因子，调控黄瓜果刺密度

FS2 encodes an ARID-HMG transcription factor that regulates fruit spine density in cucumber

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