番茄CONSTANS-LIKE基因SlCOL1通过影响GOLDEN2-LIKE蛋白的稳定调控果实叶绿素的含量

doi:10.1016/j.jia.2024.11.022

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (2): 536-545.DOI: 10.1016/j.jia.2024.11.022

番茄CONSTANS-LIKE基因SlCOL1通过影响GOLDEN2-LIKE蛋白的稳定调控果实叶绿素的含量

收稿日期:2023-11-10 接受日期:2024-05-28 出版日期:2025-02-20 发布日期:2025-01-21

The CONSTANS-LIKE SlCOL1 in tomato regulates the fruit chlorophyll content by stabilizing the GOLDEN2-LIKE protein

Long Cui^{1, 2}, Fangyan Zheng^{1, 2}, Chenhui Zhang², Sunan Gao², Jie Ye², Yuyang Zhang², Taotao Wang², Zonglie Hong³, Zhibiao Ye², Junhong Zhang^2#

¹Ganzhou Key Laboratory of Greenhouse Vegetables, College of Life Sciences, Gannan Normal University, Ganzhou 341000, China
²National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan 430070, China
³Department of Plant Sciences, University of Idaho, Moscow, Idaho 83844, USA

Received:2023-11-10 Accepted:2024-05-28 Online:2025-02-20 Published:2025-01-21
About author: #Correspondence Junhong Zhang, Tel: +86-27-87059813, Fax: +86-27-87282010, E-mail: zhangjunhng@mail.hzau.edu.cn
Supported by:
This work was supported by grants from the National Natural Science Foundation of China (32360766, 32072595 and 32202512) and the Earmarked Fund for CARS (CARS-23-A13).

摘要/Abstract

摘要：

CONSTANS（CO）和CONSTANS-LIKE（COL）转录因子可以调节植物从营养生长到成花转变等一系列过程。然而，这些转录因子在调节果实叶绿素含量方面的研究相对较少。在本研究中，发现拟南芥CONSTANS（CO）的番茄直系同源基因SlCOL1在控制番茄果实叶绿素含量方面发挥着关键作用。抑制SlCOL1基因表达，番茄未成熟绿色果实中叶绿素含量显著降低。相反，过量表达SlCOL1基因，导致番茄未成熟绿色果实中叶绿素的含量显著升高。蛋白互作结果表明，SlCOL1与GOLDEN2-LIKE（GLK2）可以直接互作并形成复合物，促进GLK2蛋白的稳定性。在番茄glk2突变体中过量表达SlCOL1并不能改变未成熟绿色果实中的叶绿素的含量，这一结果表明，SlCOL1调节叶绿素含量的重要功能需要GLK2蛋白的存在。综上所述，这些结果为COL1和GLK2调节番茄果实发育和叶绿素积累的机制提供了新的见解。

Abstract:

CONSTANS (CO) and CONSTANS-LIKE (COL) transcription factors are known to regulate a series of cellular processes, including the transition from vegetative growth to flower development in plants. However, their role in regulating the fruit chlorophyll content is poorly understood. In this study, SlCOL1, the tomato (Solanum lycopersicum) ortholog of Arabidopsis CONSTANS, was shown to play key roles in controlling fruit chlorophyll. The suppression of SlCOL1 expression led to a reduction in the chlorophyll content of immature green fruit, while the overexpression of SlCOL1 increased it. An analysis of protein–protein interactions indicated that SlCOL1 forms a complex with GOLDEN2-LIKE (GLK2), which promotes the stability of its protein. The overexpression of SlCOL1 in the glk2 null mutation background of tomato failed to promote chlorophyll accumulation in the immature green fruit, which suggests that GLK2 is required for the function of SlCOL1 in regulating chlorophyll content. These results shed new light on the mechanisms used by COL1 and GLK2 to regulate fruit development and chlorophyll accumulation in tomato.

Key words: chlorophyll , COL1 , GLK2 , BBX24 , tomato

Long Cui, Fangyan Zheng, Chenhui Zhang, Sunan Gao, Jie Ye, Yuyang Zhang, Taotao Wang, Zonglie Hong, Zhibiao Ye, Junhong Zhang. 番茄CONSTANS-LIKE基因SlCOL1通过影响GOLDEN2-LIKE蛋白的稳定调控果实叶绿素的含量[J]. Journal of Integrative Agriculture, 2025, 24(2): 536-545.

Long Cui, Fangyan Zheng, Chenhui Zhang, Sunan Gao, Jie Ye, Yuyang Zhang, Taotao Wang, Zonglie Hong, Zhibiao Ye, Junhong Zhang. The CONSTANS-LIKE SlCOL1 in tomato regulates the fruit chlorophyll content by stabilizing the GOLDEN2-LIKE protein[J]. Journal of Integrative Agriculture, 2025, 24(2): 536-545.

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番茄CONSTANS-LIKE基因SlCOL1通过影响GOLDEN2-LIKE蛋白的稳定调控果实叶绿素的含量

The CONSTANS-LIKE SlCOL1 in tomato regulates the fruit chlorophyll content by stabilizing the GOLDEN2-LIKE protein

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