生理学和转录组学分析揭示五个葡萄品种在高温下的抗旱性差异

doi:10.1016/j.jia.2024.11.006

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (8): 3055-3072.DOI: 10.1016/j.jia.2024.11.006

生理学和转录组学分析揭示五个葡萄品种在高温下的抗旱性差异

收稿日期:2024-04-16 修回日期:2024-11-04 接受日期:2024-09-27 出版日期:2025-07-20 发布日期:2025-07-16

Physiology and transcriptome profiling reveal the drought tolerance of five grape varieties under high temperatures

Xuehao Zhang¹, Qiuling Zheng², Yongjiang Hao¹, Yingying Zhang¹, Weijie Gu¹, Zhihao Deng¹, Penghui Zhou³, Yulin Fang^1#, Keqin Chen^1#, Kekun Zhang^1#

¹Heyang Viti-Viniculture Station/Ningxia Helan Mountain’s East Foothill Wine Experimentand Demonstration Station, College of Enology, Northwest A&F University, Yangling 712100, China
²Yantai Academy of Agricultural Sciences, Yantai 264000, China
³Shandong Technology Innovation Center of Wine Grape and Wine, COFCO Great Wall Wine (Penglai) Co., Ltd., Yantai 250000, China

Received:2024-04-16 Revised:2024-11-04 Accepted:2024-09-27 Online:2025-07-20 Published:2025-07-16
About author:Xuehao Zhang, E-mail: zhangxuehao@nwafu.edu.cn; #Correspondence Kekun Zhang, E-mail: zhangkekun1990@nwafu.edu.cn; Keqin Chen, E-mail: chenkeqin1985@nwafu.edu.cn; Yulin Fang, E-mail: fangyulin@nwafu.edu.cn
Supported by:
This study was supported by the Major Innovation Project of Shandong Province, China (2022CXGC010605), the National Natural Science Foundation of China (32172518 and 32002023), the National Key R&D Program of China (2023YFD2301103), and the Key R&D Projects in Ningxia Hui Autonomous Region, China (2022BBF02014).

摘要/Abstract

摘要：

高温与干旱复合胁迫下植物的抗逆性评价和关键调控基因的筛选对植物抗逆性机制的研究具有重要意义。本研究对5个葡萄品种在高温条件下的抗旱性进行了评价，筛选关键基因，进一步探索抗旱机制。通过比较和分析葡萄品种对干旱胁迫响应相关的形态特征和生理指标，并用隶属函数法对其抗旱能力进行综合评价，发现抗旱性的顺序如下：420A>110R> Cabernet Sauvignon>Fercal>188-08。为了进一步分析抗旱性差异的机制，对抗旱品种420A、干旱敏感品种188-08和对照品种CS进行了转录组测序。差异代谢途径的功能分析表明，差异表达的基因主要集中在生物过程类别中，且420A具有更高的抗氧化活性。此外，还对5个葡萄品种的差异表达的转录因子进行了分析，发现VvAGL15、VvLBD41和VvMYB86等基因与耐旱性密切相关，表明其在耐旱性调控中的潜在作用和研究意义。

Abstract:

Evaluating plant stress tolerance and screening key regulatory genes under the combined stresses of high temperature and drought are important for studying plant stress tolerance mechanisms. In this study, the drought tolerance of five grape varieties was evaluated under high-temperature conditions to screen key genes for further exploration of resistance mechanisms. By comparing and analysing the morphological characteristics and physiological indicators associated with the response of grapevines to drought stress and integrating them with the membership function to assess the strength of their drought tolerance, the order of drought tolerance was found to be as follows: 420A>110R>Cabernet Sauvignon (CS)>Fercal>188-08. To further analyse the mechanism of differences in drought tolerance, transcriptomic sequencing was performed on the drought-tolerant cultivar 420A, the drought-sensitive cultivar 188-08 and the control cultivar CS. The functional analysis of differential metabolic pathways indicated that the differentially expressed genes were enriched mainly in biological process category, that 420A had higher antioxidant activity. Furthermore, differentially expressed transcription factors were analyzed in five grape varieties. Genes like VvAGL15, VvLBD41, and VvMYB86 showed close associations with drought tolerance, indicating their potential role in regulating drought tolerance and research significance.

Key words: grape , rootstock , drought stress , drought tolerance , transcriptome

Xuehao Zhang, Qiuling Zheng, Yongjiang Hao, Yingying Zhang, Weijie Gu, Zhihao Deng, Penghui Zhou, Yulin Fang, Keqin Chen, Kekun Zhang. 生理学和转录组学分析揭示五个葡萄品种在高温下的抗旱性差异[J]. Journal of Integrative Agriculture, 2025, 24(8): 3055-3072.

Xuehao Zhang, Qiuling Zheng, Yongjiang Hao, Yingying Zhang, Weijie Gu, Zhihao Deng, Penghui Zhou, Yulin Fang, Keqin Chen, Kekun Zhang. Physiology and transcriptome profiling reveal the drought tolerance of five grape varieties under high temperatures[J]. Journal of Integrative Agriculture, 2025, 24(8): 3055-3072.

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生理学和转录组学分析揭示五个葡萄品种在高温下的抗旱性差异

Physiology and transcriptome profiling reveal the drought tolerance of five grape varieties under high temperatures

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