紫斑牡丹PrDA1-1与其互作蛋白PrTCP1/PrTCP9通过调控种子重量和数量影响产量

doi:10.1016/j.jia.2024.10.001

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (2): 610-622.DOI: 10.1016/j.jia.2024.10.001

紫斑牡丹PrDA1-1与其互作蛋白PrTCP1/PrTCP9通过调控种子重量和数量影响产量

收稿日期:2023-10-08 接受日期:2024-06-11 出版日期:2025-02-20 发布日期:2025-01-22

PrDA1-1 and its interacting proteins PrTCP1/PrTCP9 in flare tree peony affect yield by regulating seed weight and number

Chunyan He^{1, 2, 3, 4}, Fangyun Cheng^{1, 2, 3, 4#}

¹ State Key Laboratory of Efficient Production of Forest Resources, Beijing Forestry University, Beijing 100083, China
² National Engineering Research Center for Floriculture, Beijing Forestry University, Beijing 100083, China
³ Peony International Institute, Beijing Forestry University, Beijing 100083, China
⁴ School of Landscape Architecture, Beijing Forestry University, Beijing 100083, China

Received:2023-10-08 Accepted:2024-06-11 Online:2025-02-20 Published:2025-01-22
About author:Chunyan He, E-mail: hecyanw@163.com; #Correspondence Fangyun Cheng, E-mail: chengfy8@263.net
Supported by:
This work was supported by the National Key Research and Development Program of China (2023YFD1200105), the National Natural Science Foundation of China (31972446), and the 5·5 Engineering Research & Innovation Team Project of Beijing Forestry University, China (BLRC2023A06).

摘要/Abstract

摘要：

牡丹是一种新兴木本油料作物，有助于满足日益增长的食用油和生物燃料需求。目前其推广的关键是提高种子产量。DA1基因参与种子重量和数量的调控，对作物产量改良的效果明显，但其在牡丹中的功能尚未报道。本研究首次在‘京红’紫斑牡丹全基因组中鉴定出三个PrDA1基因，其中PrDA1-1为AtDA1的同源基因，包含UIM (ubiquitin-interacting motif)、LIM (Lin11, Isl- 1, and Mec-3)、DA-like三类保守结构域。PrDA1-1相对表达量在种子发育前期与后期较高，在拟南芥中组成性表达可减少种子数量、增加重量，但降低产量。PrDA1-1与PrTCP1&9在酵母和植物中均互作。在拟南芥中组成性表达PrTCP1&9增加了种子重量和数量、使产量提高。因此，我们鉴定出调控种子重量和数量的PrDA1-1和 PrTCP1&9基因，为改良牡丹种子产量提供了新的基因资源。

Abstract:

Tree peony is an emerging woody oilseed crop that has the potential to help meet the growing demand for edible oil and biofuel, and breeding high-yield varieties is the key to increasing production. The DA1 gene regulates seed weight and number and effectively improves crop yield, although its function in tree peonies has not yet been reported. In this study, three PrDA1 genes were first identified in the whole genome of ‘Jing Hong’ flare tree peony (Paeonia rockii). PrDA1-1 is homologous to AtDA1 and contains three types of conserved structural domains: UIM (ubiquitin-interacting motif), LIM (Lin11, Isl-1, and Mec-3), and DA-like. PrDA1-1 was strongly expressed during both the early and late stages of seed development, and its constitutive expression in Arabidopsis reduced seed number but increased seed weight, which ultimately led to a reduction in yield. PrDA1-1 was found to interact with PrTCP1/PrTCP9 in yeast and plants, and the constitutive expression of PrTCP1/PrTCP9 in Arabidopsis increased seed weight and number, which resulted in higher yield. Since this study showed that PrDA1-1 and PrTCP1/PrTCP9 regulate seed weight and number to affect yield, these new genetic resources will help to improve the seed yield of tree peonies.

Key words: DA1 , gene family , PrTCP1/PrTCP9 , Paeonia rockii , seed traits

Chunyan He, Fangyun Cheng. 紫斑牡丹PrDA1-1与其互作蛋白PrTCP1/PrTCP9通过调控种子重量和数量影响产量[J]. Journal of Integrative Agriculture, 2025, 24(2): 610-622.

Chunyan He, Fangyun Cheng. PrDA1-1 and its interacting proteins PrTCP1/PrTCP9 in flare tree peony affect yield by regulating seed weight and number[J]. Journal of Integrative Agriculture, 2025, 24(2): 610-622.

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紫斑牡丹PrDA1-1与其互作蛋白PrTCP1/PrTCP9通过调控种子重量和数量影响产量

PrDA1-1 and its interacting proteins PrTCP1/PrTCP9 in flare tree peony affect yield by regulating seed weight and number

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