TuABCC4基因过表达参与二斑叶螨对阿维菌素的抗药性

doi:10.1016/j.jia.2024.07.020

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (6): 2299-2310.DOI: 10.1016/j.jia.2024.07.020

TuABCC4基因过表达参与二斑叶螨对阿维菌素的抗药性

收稿日期:2024-04-25 修回日期:2024-07-18 接受日期:2024-06-04 出版日期:2025-06-20 发布日期:2025-05-13

Overexpression of TuABCC4 is associated with abamectin resistance in Tetranychus urticae Koch

Mingmei Wu*, Rui Dong*, Yan Zhang, Haojie Liao, Tian Tian, Dandan Xu, Youjun Zhang, Zhaojiang Guo^#, Shaoli Wang^#

State Key Laboratory of Vegetable Biobreeding, Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received:2024-04-25 Revised:2024-07-18 Accepted:2024-06-04 Online:2025-06-20 Published:2025-05-13
About author:Mingmei Wu, E-mail: wumingmeinongke@163.com; Rui Dong, E-mail: ruidong1526@163.com; #Correspondence Zhaojiang Guo, Tel: +86-10-82109518, E-mail: guozhaojiang@caas.cn; Shaoli Wang, Tel: +86-10-82109518, E-mail: wangshaoli@caas.cn * These authors contributed equally to this study.
Supported by:
This work was supported by the National Natural Science Foundation of China (32072458 and 32221004), the National Key R&D Program of China (2022YFD1602403), the Beijing Agriculture Innovation Consortium, China (BAIC04-2024), the earmarked fund for China Agriculture Research System (CARS-23), and the Beijing Key Laboratory for Pest Control and Sustainable Cultivation of Vegetables, China.

摘要/Abstract

摘要：

害虫对杀虫剂的抗药性严重制约了药剂的防治效果及长期使用。二斑叶螨Tetranychus urticae Koch是一种世界性范围内的重要农业害虫，已经报道对包括阿维菌素在内的多种杀虫剂产生抗药性。与阿维菌素抗性相关的研究，除谷氨酸门控氯离子通道（GluCls）导致的靶标抗性外，关于代谢抗性机制的研究仍然很有限。本研究中，我们基于前期获得的转录组和 RNA-seq数据分析，鉴定了一个在二斑叶螨抗性种群中过表达的ABCC亚家族基因TuABCC4，在敏感种群和阿维菌素抗性种群之间，该TuABCC4基因不存在稳定的非同义突变，然而在所研究的抗性种群中，TuABCC4基因的表达量均呈现显著升高。基于维拉帕米的增效剂试验以及对敏感和抗性二斑叶螨种群的基因表达量分析结果表明TuABCC4在阿维菌素抗性中发挥关键作用；此外，RNA干扰试验和遗传关联分析结果证实了TuABCC4基因的表达量升高与二斑叶螨对阿维菌素的抗性密切相关。综上所述，TuABCC4基因过表达与二斑叶螨对阿维菌素的抗性有关，这些研究结果有助于明确害虫对阿维菌素产生抗性的分子基础。

Abstract:

Pesticide resistance greatly limits control efficacy after the long-term application of pesticides. The two-spotted spider mite, Tetranychus urticae Koch, is a notorious agricultural pest worldwide that is resistant to various pesticides, including abamectin. While some studies of abamectin resistance have investigated target resistance related to glutamate-gated chloride channels (GluCls), studies on the metabolic resistance mechanisms are still limited. In this study, we identified an ABCC subfamily gene, TuABCC4, that was overexpressed in resistant populations of T. urticae, based on the analysis of previously obtained transcriptomic and RNA-seq data. No consistent nonsynonymous mutations in the TuABCC4 gene were found between the susceptible and resistant populations, although TuABCC4 expression was significantly increased in all the resistant populations that were studied. Synergistic experiments with the inhibitor verapamil and gene expression analysis of the susceptible and resistant populations confirmed the key role of TuABCC4 in abamectin resistance. In addition, an increase in the expression of the TuABCC4 gene was shown by RNA interference and genetic association analysis to be closely related to the resistance of T. urticae to abamectin. In conclusion, overexpression of TuABCC4 was shown to be involved in abamectin resistance in T. urticae. These results can help us to better understand the molecular basis of pest resistance to abamectin.

Key words: Tetranychus urticae , ABC transporter gene , resistance mechanism , RNA interference , genetic association analysis

Mingmei Wu, Rui Dong, Yan Zhang, Haojie Liao, Tian Tian, Dandan Xu, Youjun Zhang, Zhaojiang Guo, Shaoli Wang. TuABCC4基因过表达参与二斑叶螨对阿维菌素的抗药性[J]. Journal of Integrative Agriculture, 2025, 24(6): 2299-2310.

Mingmei Wu, Rui Dong, Yan Zhang, Haojie Liao, Tian Tian, Dandan Xu, Youjun Zhang, Zhaojiang Guo, Shaoli Wang.

Overexpression of TuABCC4 is associated with abamectin resistance in Tetranychus urticae Koch

[J]. Journal of Integrative Agriculture, 2025, 24(6): 2299-2310.

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TuABCC4基因过表达参与二斑叶螨对阿维菌素的抗药性

Overexpression of TuABCC4 is associated with abamectin resistance in Tetranychus urticae Koch

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