番茄中MAP65基因家族成员鉴定及其成员SlMAP65-1参与果实形态建成

doi:10.1016/j.jia.2024.07.014

摘要/Abstract

摘要：

植物的生长发育过程伴随着微管的动态变化，而微管阵列的重排受多种微管结合蛋白（microtubule-associated proteins , MAPs）的调控。植物中的MAP65蛋白在一些物种中已经得到了详尽的表征，但有关番茄（Solanum lycopersicum）中 MAP65 家族成员的信息还很有限。本研究在番茄基因组中鉴定到了 9 个 SlMAP65 基因家族成员。对 SlMAP65 家族成员的理化性质、亲缘关系、保守基序、结构域、基因结构和顺式调控元件等进行了系统分析。然后利用 CRISPR/Cas9 技术在番茄中敲除了相对表达量最高的成员SlMAP65-1。结果表明，slmap65-1突变体果实果形指数显著增加，SlMAP65-1在果实发育早期参与了果实的形态发生。这些结果为番茄果实形态建成相关研究领域以及未来SlMAP65家族成员的功能研究提供了新信息。

Abstract:

The plant growth process is accompanied by dynamic changes in the microtubules, and the rearrangement of microtubules is regulated by diverse microtubule-associated proteins (MAPs). Plant MAP65s have been exhaustively characterized in some species, but the information about MAP65 family members in tomato (Solanum lycopersicum) is limited. In this study, nine SlMAP65 family genes were identified in the tomato genome. Then a systematic analysis that considered the physio-chemical properties, evolution, conserved motifs, domains, gene structure, and cis-regulatory elements of SlMAP65 family members was conducted. The family member SlMAP65-1, which had the highest expression, was knocked out by CRISPR/Cas9. The tomato fruit of slmap65-1 loss of function lines showed an elongated morphology, and the data indicated that SlMAP65-1 is involved in fruit morphogenesis at the early fruit development stage. These results provide new insights for fruit morphogenesis-related research and future functional studies of the SlMAP65 family members in tomato.

Key words: tomato , MAP65 family , fruit shape , microtubule organization

Peiyu Zhang, Guoning Zhu, Chunjiao Zhang, Hongliang Zhu. 番茄中MAP65基因家族成员鉴定及其成员SlMAP65-1参与果实形态建成[J]. Journal of Integrative Agriculture, 2025, 24(2): 564-574.

Peiyu Zhang, Guoning Zhu, Chunjiao Zhang, Hongliang Zhu. Functional analysis of tomato MAP65 gene family, highlighting SlMAP65-1’s role in fruit morphogenesis[J]. Journal of Integrative Agriculture, 2025, 24(2): 564-574.

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