绿豆种皮颜色相关候选基因的精细定位及初步分析

doi:10.1016/j.jia.2024.04.022

摘要/Abstract

摘要：

绿豆种皮色与种子的外观品质及商品性密切相关。影响绿豆种皮色的物质主要为黄酮类化合物，具有重要的药用价值。定位绿豆种皮颜色基因将有助于开发新品种，提高其应用价值。本研究利用冀绿9号（黑色种皮）和BIS9805（绿色种皮）构建了一个由546株后代组成的F₂定位群体。利用BSA-seq方法和KASP分子标记，将种皮颜色相关的候选区域定位在4号染色体上，最终缩小到0.66 Mb区间之内，包含8个候选基因。结合转录组和代谢组分析发现，8个候选基因中有3个基因（LOC106758748、LOC106758747和LOC106759075）差异表达，可能导致冀绿9号和BIS9805之间类黄酮代谢物含量差异。这些研究结果为绿豆种皮颜色相关基因的克隆和加快分子标记辅助选择育种提供了理论依据。

Abstract:

Pre-harvest sprouting (PHS) poses a significant global challenge to cereal production, impacting both yield and quality. Seed coat color affects the appearance and commodity quality of mung beans (Vigna radiata L.). The substances that affect mung bean seed coat color are mainly flavonoids, which have important medicinal value. Mapping the seed coat color gene in mung beans would facilitate the development of new varieties and improve their value. In this study, an F₂mapping population consisting of 546 plants was constructed using Jilv9 (black seed coat) and BIS9805 (green seed coat). Using bulk segregated analysis (BSA) sequencing and kompetitive allele-specific PCR (KASP) markers, the candidate region related to seed coat color was finally narrowed to 0.66 Mb on chromosome (Chr.) 4 and included eight candidate genes. Combined transcriptome and metabolome analyses showed that three of the eight candidate genes (LOC106758748, LOC106758747, and LOC106759075) were differentially expressed, which may have caused the differences in flavonoid metabolite content between Jilv9 and BIS9805. These findings can provide a research basis for cloning the genes related to seed coat color and accelerate molecular marker-assisted selection breeding in mung beans.

Key words: mung bean , seed coat color , bulk segregated analysis sequencing , transcriptome , metabolism , flavonoids

Qian Wang, Huimin Cao, Jingcheng Wang, Zirong Gu, Qiuyun Lin, Zeyan Zhang, Xueying Zhao, Wei Gao, Huijun Zhu, Hubin Yan, Jianjun Yan, Qingting Hao, Yaowen Zhang. 绿豆种皮颜色相关候选基因的精细定位及初步分析[J]. Journal of Integrative Agriculture, 2024, 23(8): 2571-2588.

Qian Wang, Huimin Cao, Jingcheng Wang, Zirong Gu, Qiuyun Lin, Zeyan Zhang, Xueying Zhao, Wei Gao, Huijun Zhu, Hubin Yan, Jianjun Yan, Qingting Hao, Yaowen Zhang. Fine-mapping and primary analysis of candidate genes associated with seed coat color in mung bean (Vigna radiata L.)[J]. Journal of Integrative Agriculture, 2024, 23(8): 2571-2588.

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