抑制茶树CsFAD3通过介导JA而非SA途径降低茶树耐旱性

doi:10.1016/j.jia.2024.04.002

摘要/Abstract

摘要：

干旱胁迫严重限制着茶树生长以及产量。脂肪酸去饱和酶(FADs)通过调节细胞膜流动性应对不同胁迫，然而，关于ω-3脂肪酸去饱和酶(ω-3 FADs)在缓解茶树干旱胁迫的作用机制尚不清楚。本研究发现干旱胁迫处理后显著增加C18:3 (亚麻酸)百分含量和CsFAD3表达量；通过酵母实验证明CsFAD3 具有将C18:2 转化为C18:3功能，亚细胞定位实验证明其定位于内质网。在茶树中瞬时沉默CsFAD3，其Fv/Fm减少、MDA含量增加，因此抗旱性显著降低；然而，转基因35S:CsFAD3拟南芥植株表现出抗旱的表型。此外，在干旱胁迫下，瞬时沉默茶树CsFAD3，茶树叶片中JA含量及CsLOX2、CsLOX4、CsAOS、CsAOC3和CsOPR的表达量均显著降低；然而SA含量无显著变化。对35S:CsFAD3背景下的拟南芥突变株Atcoil1 (JA受体)或AtNPR1 (SA受体)的分析进一步发现，敲除Atcoil1使过表达的CsFAD3株系的耐旱表型受损。因此，我们的研究结果表明，CsFAD3通过介导JA通路在抗旱性中发挥了至关重要的作用。

Abstract:

The growth and yield of tea plants are seriously limited by drought stress. Fatty acid desaturases (FADs) contribute to the mediation of membrane fluidity in response to different stresses, although the role of ω-3 FAD (Omega-3 fatty acid desaturase)-mediated damage induced by drought stress in tea plants is poorly understood. In this study, drought stress significantly promoted the synthesis of C18:3 (linolenic acid) and the expression level of CsFAD3. Yeast experiments further demonstrated that CsFAD3 can convert C18:2 to C18:3, and that the 35S:GFP-CsFAD3 fusion protein was localized in the endoplasmic reticulum of Nicotiana benthamiana cells. CsFAD3-silenced tea leaves exhibited poor drought tolerance, with a lower F_v/F_m and a higher malondialdehyde (MDA) content than the control plants. However, transgenic 35S:CsFAD3 Arabidopsis plants showed the opposite phenotypes. In addition, the jasmonic acid (JA) content and the expression levels of CsLOX2, CsLOX4, CsAOS, CsAOC3 and CsOPR2 were significantly reduced in CsFAD3-silenced leaves under drought stress. However, no substantial difference in the salicylic acid (SA) content was detected under normal or drought conditions. An analysis of Atcoi1 (JA receptor) or Atnpr1 (SA receptor) mutant Arabidopsis plants in 35S:CsFAD3 backgrounds further revealed that knockout of Atcoi1 impaired the drought-tolerant phenotypes of CsFAD3 overexpression lines. Therefore, this study demonstrated that CsFAD3 plays a crucial role in drought tolerance by mediating JA pathways.

Key words: tea plants , CsFAD3 , drought stress , jasmonic acid

Na Chang, Xiaotian Pi, Ziwen Zhou, Yeyun Li, Xianchen Zhang. 抑制茶树CsFAD3通过介导JA而非SA途径降低茶树耐旱性[J]. Journal of Integrative Agriculture, 2024, 23(11): 3737-3750.

Na Chang, Xiaotian Pi, Ziwen Zhou, Yeyun Li, Xianchen Zhang. Suppression of CsFAD3 in a JA-dependent manner, but not through the SA pathway, impairs drought stress tolerance in tea[J]. Journal of Integrative Agriculture, 2024, 23(11): 3737-3750.

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