属间染色体特异涂染探针揭示甘蔗杂交后代中斑茅染色体遗传差异

doi:10.1016/j.jia.2024.08.019

Journal of Integrative Agriculture ›› 2024, Vol. 23 ›› Issue (11): 3751-3762.DOI: 10.1016/j.jia.2024.08.019

属间染色体特异涂染探针揭示甘蔗杂交后代中斑茅染色体遗传差异

收稿日期:2023-05-02 接受日期:2024-06-17 出版日期:2024-11-20 发布日期:2024-10-10

Intergeneric chromosome-specific painting reveals differential chromosomal transmission from Tripidium arundinaceum in sugarcane progeny

Fan Yu^1*, Zehuai Yu^1*, Jin Chai^2*, Xikai Yu¹, Chen Fu³, Xinwang Zhao^{1, 2, 4}, Hailong Chang³, Jiawei Lei², Baoshan Chen¹, Wei Yao¹, Muqing Zhang^1#, Jiayun Wu^3#, Qinnan Wang^3#, Zuhu Deng^{1, 2, 4#}

¹State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangxi Key Laboratory for Sugarcane Biology, College of Agriculture, Guangxi University, Nanning 530004, China
²National Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University, Fuzhou 350002, China
³Institute of Nanfan & Seed Industry, Guangdong Academy of Sciences, Guangzhou 510316, China
⁴Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs/Fujian Agriculture and Forestry University, Fuzhou 350002, China

Received:2023-05-02 Accepted:2024-06-17 Online:2024-11-20 Published:2024-10-10
About author:Fan Yu, E-mail: yufanky@163.com; Zehuai Yu, E-mail: arhuay_yu@163.com; Jin Chai, E-mail: CJ1152648@163.com; #Correspondence Zuhu Deng, E-mail: dengzuhu@163.com; Qinnan Wang, E-mail: wangqinnan66@163.com; Jiayun Wu, E-mail: jiayunng@163.com; Muqing Zhang, E-mail: zmuqing@163.com *These authors contributed equally to this study.
Supported by:
This research was funded by the Central Government and Local Science and Technology Development Special Project, China (2022L3086) and the Sugarcane Research Foundation of Guangxi University, China (2022GZB006). This project was also supported by the National Natural Science Foundation of China (31771863), the Academy of Sugarcane and Sugar Industry, Guangxi University, China (ASSI-2023009), an independent fund of Guangxi Key Laboratory of Sugarcane Biology, China (GXKLSCB-20190201) and the China Agriculture Research System of MOF and MARA (CARS-20-1-5).

摘要/Abstract

摘要：

甘蔗近年来作为制糖和生物乙醇的原料引起了越来越多的关注，提高它的产量对保证食糖安全和生物能源生产至关重要。属间远缘杂交是作物产生遗传变异的高效途径之一，尤其是高倍体作物甘蔗。斑茅因其具有许多优良的农艺性状，已被广泛研究用于改良甘蔗的抗旱性和其它抗逆性。然而，甘蔗属物种和斑茅间的亲缘关系以及甘蔗杂交后代中斑茅染色体的组成都仍不清晰。与先前利用叶绿体基因组DNA进行的遗传分析不同，本研究基于不同物种全基因组单核苷酸多态性明确了斑茅与甘蔗的亲缘关系比高粱更近。此外，利用热带种基因组设计的寡核苷酸染色体特异涂染探针能够清晰地识别斑茅的染色体。本研究首次建立的寡聚核苷酸基因组原位杂交体系，可用于检测甘蔗杂交后代中斑茅染色体的易位和单条染色体遗传。值得注意的是，我们还发现在BC₁子代中斑茅的染色体出现了n, 2n和超2n的遗传方式。这些遗传方式的不同可能是由于减数第一次分裂染色体加倍、减数第一次分裂和第二次分裂染色体加倍或减数第二次分裂时姐妹染色单体不分离所导致的。这些结果将为进一步选择斑茅染色体进行甘蔗遗传改良提供重要依据。

Abstract:

Sugarcane has recently attracted increasing attention for its potential as a source of sugar and bioethanol, so increasing its yield is essential to ensure the sugar security and bioenergy production. Intergeneric hybridization is a highly efficient method to produce new genetic variants of crop plants, particularly those species with high ploidy such as sugarcane (Saccharum spp.). Tripidium arundinaceum exhibits many desirable agronomic traits, and has been widely studied to produce hybrids with improved stress tolerance and other characteristics in sugarcane breeding. However, the genetic relationship between T. arundinaceum and Saccharum species, and the individual T. arundinaceum chromosomal compositions in sugarcane hybrids are still elusive. Here we used whole-genome single-nucleotide polymorphisms (SNPs) to ascertain the phylogenetic relationships between these species and found that T. arundinaceum is more closely related to Saccharum than Sorghum, in contrast to the previous narrow genetic analyses using chloroplast DNA. Additionally, oligonucleotide (oligo)-based chromosome-specific painting derived from Saccharum officinarum was able to distinctly identify the chromosomes of T. arundinaceum. We developed the oligo-genomic in situ hybridization (GISH) system for the first time, to unveil the novel chromosome translocations and the transmission of individual T. arundinaceum chromosomes in sugarcane progeny. Notably, we discovered that the chromosomal transmission of T. arundinaceum exhibited several different inheritance modes, including n, 2n, and over 2n in the BC₁ progenies. Such inheritance patterns may have resulted from first division restitution (FDR) or FDR+nondisjunction of a chromosome with the sister chromatids in the second meiosis division/second division restitution (FDR+NSC/SDR) model during meiosis. These results will be of substantial benefit for the further selection of T. arundinaceum chromosomes for sugarcane genetic improvement.

Key words: sugarcane , Tripidium arundinaceum , chromosome painting , Oligo-FISH , chromosomal transmission

Fan Yu, Zehuai Yu, Jin Chai, Xikai Yu, Chen Fu, Xinwang Zhao, Hailong Chang, Jiawei Lei, Baoshan Chen, Wei Yao, Muqing Zhang, Jiayun Wu, Qinnan Wang, Zuhu Deng. 属间染色体特异涂染探针揭示甘蔗杂交后代中斑茅染色体遗传差异[J]. Journal of Integrative Agriculture, 2024, 23(11): 3751-3762.

Fan Yu, Zehuai Yu, Jin Chai, Xikai Yu, Chen Fu, Xinwang Zhao, Hailong Chang, Jiawei Lei, Baoshan Chen, Wei Yao, Muqing Zhang, Jiayun Wu, Qinnan Wang, Zuhu Deng. Intergeneric chromosome-specific painting reveals differential chromosomal transmission from Tripidium arundinaceum in sugarcane progeny[J]. Journal of Integrative Agriculture, 2024, 23(11): 3751-3762.

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属间染色体特异涂染探针揭示甘蔗杂交后代中斑茅染色体遗传差异

Intergeneric chromosome-specific painting reveals differential chromosomal transmission from Tripidium arundinaceum in sugarcane progeny

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