一个新的组蛋白甲基转移酶基因CgSDG40正调控柑橘果实成熟过程中类胡萝卜素的生物合成

doi:10.1016/j.jia.2024.03.068

Journal of Integrative Agriculture ›› 2024, Vol. 23 ›› Issue (8): 2633-2648.DOI: 10.1016/j.jia.2024.03.068

一个新的组蛋白甲基转移酶基因CgSDG40正调控柑橘果实成熟过程中类胡萝卜素的生物合成

收稿日期:2023-07-07 接受日期:2023-11-27 出版日期:2024-08-20 发布日期:2024-07-29

A novel histone methyltransferase gene CgSDG40 positively regulates carotenoid biosynthesis during citrus fruit ripening

Jialing Fu^{1, 2*}, Qingjiang Wu^{1, 3*}, Xia Wang^{1, 2}, Juan Sun¹, Li Liao¹, Li Li^{4, 5}, Qiang Xu^{1, 2#}

¹ National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture & Forestry Science, Huazhong Agricultural University, Wuhan 430070, China
² Hubei Hongshan Laboratory, Wuhan 430070, China
³ Institute of Horticultural Sciences, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China
⁴ Plant Breeding and Genetics Section, School of Integrative Plant Science, Cornell University, New York 14853, USA
⁵Robert W. Holley Center for Agriculture and Health, USDA-Agricultural Research Service, Cornell University, New York 14853, USA

Received:2023-07-07 Accepted:2023-11-27 Online:2024-08-20 Published:2024-07-29
About author:Jialing Fu, E-mail: fujialing96@163.com; Qingjiang Wu, E-mail: wqj9319@126.com; #Correspondence Qiang Xu, Tel: +86-27-87286623, E-mail: xuqiang@mail.hzau.edu.cn * These authors contributed equally to this study.
Supported by:
This work was supported by the Major Special Projects and Key R&D Projects in Yunnan Province, China (202102AE090054), the National Natural Science Foundation of China (31925034), the Foundation of Hubei Hongshan Laboratory granted to Dr. Qiang Xu, China (2021hszd016), and the Key Project of Hubei Provincial Natural Science Foundation, China (2021CFA017).

摘要/Abstract

摘要：

【目的】柚子(Citrus maxima)丰富多样的果肉色泽由对人体健康有益的类胡萝卜素的组成和含量所决定。因此，研究类胡萝卜素生物合成的调控网络对提高柚果实中类胡萝卜素的含量具有重要意义。然而，目前柚果实中类胡萝卜素合成代谢的调控分子机制尚不完全清楚。【方法】在本研究中，通过分析典型的红肉柚和白肉柚以及杂交分离群体F1代的果肉颜色极端单株的果肉转录组数据，我们鉴定到一个新的组蛋白甲基转移酶基因CgSDG40。【结果】CgSDG40的表达与果肉颜色高度相关，且与类胡萝卜素合成关键限速酶基因CgPSY1的表达呈现出共表达趋势。有趣的是，CgSDG40和CgPSY1在染色体上的位置是以“头对头”的形式紧挨着的，且共享一段有重叠的启动子区域。亚细胞定位实验表明CgSDG40定位于细胞核。在柑橘愈伤中超量表达CgSDG40可以显著提高愈伤中类胡萝卜素的含量，同时愈伤中CgPSY1的表达也显著提高。【结论与意义】综上所述，我们的研究揭示了一个新的组蛋白甲基转移酶基因CgSDG40参与了类胡萝卜素的生物合成调控，这为分子设计育种和果实色泽与营养品质的遗传育种改良提供了新的理论基础。

Abstract:

The flesh color of pummelo (Citrus maxima) fruits is highly diverse and largely depends on the level of carotenoids, which are beneficial to human health. It is vital to investigate the regulatory network of carotenoid biosynthesis to improve the carotenoid content in pummelo. However, the molecular mechanism underlying carotenoid accumulation in pummelo is not fully understood. In this study, we identified a novel histone methyltransferase gene, CgSDG40, involved in carotenoid regulation by analyzing the flesh transcriptome of typical white-fleshed pummelo, red-fleshed pummelo and extreme-colored F₁ hybrids from a segregated pummelo population. Expression of CgSDG40 corresponded to flesh color change and was highly coexpressed with CgPSY1. Interestingly, CgSDG40 and CgPSY1 are located physically adjacent to each other on the chromosome in opposite directions, sharing a partially overlapping promoter region. Subcellular localization analysis indicated that CgSDG40 localizes to the nucleus. Overexpression of CgSDG40 significantly increased the total carotenoid content in citrus calli relative to that in wild type. In addition, expression of CgPSY1 was significantly activated in overexpression lines relative to wild type. Taken together, our findings reveal a novel histone methyltransferase regulator, CgSDG40, involved in the regulation of carotenoid biosynthesis in citrus and provide new strategies for molecular design breeding and genetic improvement of fruit color and nutritional quality.

Key words: pummelo , SET domain protein , epigenetic regulation , PSY1 , lycopene , fruit quality , fruit color

Jialing Fu, Qingjiang Wu, Xia Wang, Juan Sun, Li Liao, Li Li, Qiang Xu. 一个新的组蛋白甲基转移酶基因CgSDG40正调控柑橘果实成熟过程中类胡萝卜素的生物合成[J]. Journal of Integrative Agriculture, 2024, 23(8): 2633-2648.

Jialing Fu, Qingjiang Wu, Xia Wang, Juan Sun, Li Liao, Li Li, Qiang Xu. A novel histone methyltransferase gene CgSDG40 positively regulates carotenoid biosynthesis during citrus fruit ripening[J]. Journal of Integrative Agriculture, 2024, 23(8): 2633-2648.

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一个新的组蛋白甲基转移酶基因CgSDG40正调控柑橘果实成熟过程中类胡萝卜素的生物合成

A novel histone methyltransferase gene CgSDG40 positively regulates carotenoid biosynthesis during citrus fruit ripening

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