2020至2022年间中国中部地区H9N2亚型禽流感病毒分离株的遗传演化及生物学特性研究

doi:10.1016/j.jia.2024.03.055

Journal of Integrative Agriculture ›› 2024, Vol. 23 ›› Issue (8): 2778-2791.DOI: 10.1016/j.jia.2024.03.055

2020至2022年间中国中部地区H9N2亚型禽流感病毒分离株的遗传演化及生物学特性研究

收稿日期:2023-11-29 接受日期:2024-02-07 出版日期:2024-08-20 发布日期:2024-07-29

Genetic and biological properties of H9N2 avian influenza viruses isolated in central China from 2020 to 2022

Libin Liang^1*#, Yaning Bai^1*, Wenyan Huang^1*, Pengfei Ren^1*, Xing Li¹, Dou Wang¹, Yuhan Yang¹, Zhen Gao¹, Jiao Tang¹, Xingchen Wu¹, Shimin Gao¹, Yanna Guo¹, Mingming Hu², Zhiwei Wang², Zhongbing Wang², Haili Ma^1#, Junping Li^1#

¹College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China
² Shanxi Provincial Animal Disease Prevention and Control Center, Taiyuan 030000, China

Received:2023-11-29 Accepted:2024-02-07 Online:2024-08-20 Published:2024-07-29
About author:#Correspondence Libin Liang, Tel: +86-354-6285988, E-mail: lianglibin521@126.com; Junping Li, Tel: +86-354-6285988, E-mail: lijunping916@163.com; Haili Ma, Tel: +86-354-6288629, E-mail: mahaili1718@126.com * These authors contributed equally to this study.
Supported by:
This work was supported by the Fundamental Research Program of Shanxi Province, China (202103021224156), the National Natural Science Foundation of China (32202788), the Special Research Fund of Shanxi Agricultural University for High-level Talents, China (2021XG004), the special fund for Science and Technology Innovation Teams of Shanxi Province, China (202304051001041), the Science and Technology Innovation Program of Shanxi Agricultural University, China (2021BQ78), the Shanxi Province Excellent Doctoral Work Award-Scientific Research Project, China (SXBYKY2021005, SXBYKY2021063, SXBYKY2022014), the Fund for Shanxi “1331 Project”, China (20211331-13), the earmarked fund for Modern Agro-industry Technology Research System of Shanxi Province, China.

摘要/Abstract

摘要：

H9N2亚型禽流感病毒(AIV)在全球范围内的家禽和野鸟中广泛流行，已成为我国家禽中流行的主要亚型。H9N2 AIV可以直接感染人，并且可以为近年来出现的H5N6、H7N9、H3N8等直接跨种传播感染人的毒株提供内部基因，对公共卫生安全构成重大威胁。目前，针对中国山西地区H9N2 AIV的流行情况、遗传演化、对家禽和哺乳动物的致病性、抗原性变异情况等缺乏深入研究。本研究对2020年至2022年在山西省分离到的14株H9N2病毒进行了系统的遗传演化及生物学特性分析。全基因组序列测定及遗传进化分析显示这些H9N2病毒可以划分为7个不同的基因型，不同毒株的基因片段来源复杂多样。动物实验结果表明，本研究分离到的在鸡群中具有高效传播效率，并且在鸡体内具有不同的复制能力；H9N2 毒株可以在小鼠的肺部进行高水平复制，尤其值得关注的是其中一株病毒可以在小鼠的大脑和肾脏等器官中复制，提示其在哺乳动物体内的组织嗜性及致病性进一步增强。在体外细胞水平，利用噬斑形成试验及多步生长曲线试验评估了不同H9N2毒株的复制能力，发现不同H9N2病毒在细胞水平的复制和增殖效率同样具有明显差异。进一步利用双荧光素酶报告系统评估了在体内及细胞水平复制能力均差异明显的两株H9N2病毒的聚合酶活性，发现复制能力强的毒株具有更高的聚合酶活性，而且这种高聚合酶活性主要与二者的PB2及NP基因有关。为评估H9N2分离株的抗原性是否有差异，在利用SPF鸡制备抗血清的基础上，应用交叉血凝抑制试验进行了抗原性分析。抗原性分析结果表明，这些H9N2分离株形成了两个独立的抗原群，抗原性存在显著差异。本研究从H9N2病毒遗传演化、动物体内的复制能力及致病性、体外细胞水平复制能力、抗原性变异情况等，对山西地区H9N2病毒进行了系统研究，为了解我国中部地区H9N2 AIV的流行情况及生物学特性提供了重要参考。

Abstract:

The H9N2 subtype of avian influenza virus (AIV) is widely prevalent in poultry and wild birds globally, and has become the predominant subtype circulating in poultry in China. The H9N2 AIV can directly or indirectly (by serving as a “donor virus”) infect humans, posing a significant threat to public health. Currently, there is a lack of in-depth research on the prevalence of H9N2 viruses in Shanxi Province, central China. In this study, we isolated 14 H9N2 AIVs from October 2020 to April 2022 in Shanxi Province, and genetic analysis revealed that these viruses belonged to 7 different genotypes. Our study on animals revealed that the H9N2 strains we identified displayed high transmission efficiency among chicken populations, and exhibited diverse replication abilities within these birds. These viruses could replicate efficiently in the lungs of mice, with one strain also demonstrating the capacity to reproduce in organs like the brain and kidneys. At the cellular level, the replication ability of different H9N2 strains was evaluated using plaque formation assays and multi-step growth curve assays, revealing significant differences in the replication and proliferation efficiency of the various H9N2 viruses at the cellular level. The antigenicity analysis suggested that these isolates could be classified into 2 separate antigenic clusters. Our research provides crucial data to help understand the prevalence and biological characteristics of H9N2 AIVs in central China. It also highlights the necessity of enhancing the surveillance of H9N2 AIVs.

Key words: avian influenza virus , H9N2 , central China , pathogenicity , antigenicity

Libin Liang, Yaning Bai, Wenyan Huang, Pengfei Ren, Xing Li, Dou Wang, Yuhan Yang, Zhen Gao, Jiao Tang, Xingchen Wu, Shimin Gao, Yanna Guo, Mingming Hu, Zhiwei Wang, Zhongbing Wang, Haili Ma, Junping Li. 2020至2022年间中国中部地区H9N2亚型禽流感病毒分离株的遗传演化及生物学特性研究[J]. Journal of Integrative Agriculture, 2024, 23(8): 2778-2791.

Libin Liang, Yaning Bai, Wenyan Huang, Pengfei Ren, Xing Li, Dou Wang, Yuhan Yang, Zhen Gao, Jiao Tang, Xingchen Wu, Shimin Gao, Yanna Guo, Mingming Hu, Zhiwei Wang, Zhongbing Wang, Haili Ma, Junping Li. Genetic and biological properties of H9N2 avian influenza viruses isolated in central China from 2020 to 2022[J]. Journal of Integrative Agriculture, 2024, 23(8): 2778-2791.

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2020至2022年间中国中部地区H9N2亚型禽流感病毒分离株的遗传演化及生物学特性研究

Genetic and biological properties of H9N2 avian influenza viruses isolated in central China from 2020 to 2022

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