利用转录组学和代谢组学综合分析外源亚精胺缓解玉米渍涝胁迫的机制

doi:10.1016/j.jia.2024.03.041

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (12): 4546-4560.DOI: 10.1016/j.jia.2024.03.041

利用转录组学和代谢组学综合分析外源亚精胺缓解玉米渍涝胁迫的机制

收稿日期:2023-11-15 修回日期:2024-03-11 接受日期:2024-01-17 出版日期:2025-12-20 发布日期:2025-11-13

Integrated transcriptomics and metabolomics analysis provide insights into the alleviation of waterlogging stress in maize by exogenous spermidine application

Xiuling Wang^1*, Li Niu^2*, Huaipan Liu³, Xucun Jia¹, Yulong Zhao⁴, Qun Wang¹, Yali Zhao¹, Pengfei Dong¹, Moubiao Zhang¹, Hongping Li¹, Panpan An¹, Zhi Li^{1, 5}, Xiaohuan Mu^1#, Yongen Zhang^{1, 5#}, Chaohai Li^1#

¹State Key Laboratory of High-Efficiency Production of Wheat–Maize Double Cropping/Collaborative Innovation Center of Henan Grain Crops/College of Agronomy, Henan Agricultural University, Zhengzhou 450046, China
²Beijing Changping Agricultural Technology Extension Station, Beijing 102200, China
³College of Life Science and Agronomy/Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal University, Zhoukou 466001, China
⁴College of Forestry, Henan Agricultural University, Zhengzhou 450046, China
⁵Agricultural Information Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received:2023-11-15 Revised:2024-03-11 Accepted:2024-01-17 Online:2025-12-20 Published:2025-11-13
About author:Xiuling Wang, E-mail: 15038348221@163.com; Li Niu, E-mail: niulipipi@163.com; #Correspondence Chaohai Li, E-mail: lichaohai@henau.edu.cn; Xiaohuan Mu, E-mail: xiaohuanmu@henau.edu.cn; Yongen Zhang, Tel: +86-10-82109654, E-mail: zhangyongen@caas.cn * These authors contributed equally to this study.
Supported by:
This research was supported by the China Agriculture Research System (CARS-02-20) and the Henan Province Agro-ecosystem Field Observation and Research Station, China (30602535).

摘要/Abstract

摘要：

渍涝胁迫会影响植物的生发发育，进而降低作物产量。亚精胺(Spd)作为第二信使，在渍涝胁迫下对植物的生长有缓解作用。然而，外源施加Spd缓解渍涝胁迫的分子机制尚不清楚。本研究利用生理学分析和多组学联合分析以揭示施加Spd对渍涝胁迫的影响。施加Spd使参与捕光复合体(LHC)、光合作用和淀粉合成相关途径的基因上调表达，同时抑制了叶绿素降解，因此保持了较高的光合速率，从而增加了淹水胁迫下生物量的积累；海藻糖和Spd生物合成相关基因的上调表达引起海藻糖和内源性Spd的积累；抑制1-氨基环丙烷-1-羧酸氧化酶(ACO)基因的表达有助于减少乙烯排放。这些变化增加了玉米对渍涝的抗性。外源施加Spd后，生长素相关的基因发生上调表达，同时IAA含量增加，有利于玉米细胞伸长，能够使玉米在涝渍胁迫下保持相对正常的生产。在渍涝胁迫下施加外源Spd使大部分参与脂质合成相关的基因发生上调表达，引起脂质的积累，保护了细胞膜的完整性和稳定性。这些改变均有利于抵抗涝渍胁迫。本研究的发现扩宽了我们对外源施用Spd缓解渍涝损害机制的理解，并为培育耐涝玉米品种提供证据。

Abstract:

Waterlogging stress significantly impairs plant growth and reduces crop yields. Spermidine (Spd), functioning as a second messenger, demonstrates positive effects on plant growth under waterlogging stress conditions. However, the molecular mechanisms by which exogenous Spd application alleviates waterlogging stress remain unclear. This study employed physiological analysis and multi-omics approaches to investigate the effect of Spd application on waterlogging stress. The application of Spd enhanced the expression of genes related to light-harvesting complex (LHC), photosynthesis, and starch-related pathways, while inhibiting chlorophyll degradation and maintaining higher photosynthetic rates, thereby increasing biomass accumulation under waterlogging stress. The activation of genes associated with trehalose and Spd biosynthesis resulted in elevated accumulation of trehalose and endogenous Spd. The inhibition of 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO) expression contributed to reduced ethylene emission, enhancing maize resistance to waterlogging. Following Spd application, auxin-related genes were up-regulated and indole acetic acid (IAA) content increased, promoting cell elongation in maize and maintaining normal growth under waterlogging stress. Additionally, the upregulation of lipid-related genes led to increased lipid content, protecting cell membranes under waterlogging conditions. These molecular and physiological modifications collectively enhanced resistance to waterlogging stress. These findings advance our understanding of Spd’s regulatory roles in mitigating waterlogging damage and provide valuable insights for breeding waterlogging-tolerant maize varieties.

Key words: maize , spermidine , waterlogging stress , transcriptome , metabolome

Xiuling Wang, Li Niu, Huaipan Liu, Xucun Jia, Yulong Zhao, Qun Wang, Yali Zhao, Pengfei Dong, Moubiao Zhang, Hongping Li, Panpan An, Zhi Li, Xiaohuan Mu, Yongen Zhang, Chaohai Li. 利用转录组学和代谢组学综合分析外源亚精胺缓解玉米渍涝胁迫的机制[J]. Journal of Integrative Agriculture, 2025, 24(12): 4546-4560.

Xiuling Wang, Li Niu, Huaipan Liu, Xucun Jia, Yulong Zhao, Qun Wang, Yali Zhao, Pengfei Dong, Moubiao Zhang, Hongping Li, Panpan An, Zhi Li, Xiaohuan Mu, Yongen Zhang, Chaohai Li. Integrated transcriptomics and metabolomics analysis provide insights into the alleviation of waterlogging stress in maize by exogenous spermidine application[J]. Journal of Integrative Agriculture, 2025, 24(12): 4546-4560.

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利用转录组学和代谢组学综合分析外源亚精胺缓解玉米渍涝胁迫的机制

Integrated transcriptomics and metabolomics analysis provide insights into the alleviation of waterlogging stress in maize by exogenous spermidine application

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