H8N4禽流感病毒的进化和生物学特性

doi:10.1016/j.jia.2023.12.033

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (6): 2342-2355.DOI: 10.1016/j.jia.2023.12.033

H8N4禽流感病毒的进化和生物学特性

收稿日期:2023-07-31 修回日期:2024-01-03 接受日期:2023-11-24 出版日期:2025-06-20 发布日期:2025-05-13

Evolution and biological characteristics of the circulated H8N4 avian influenza viruses

Hong Zhang^1*, Jing Guo^1*, Peng Peng^2*, Mengjing Wang¹, Jinyan Shen¹, Xiaohong Sun¹, Mengdi Guan¹, Pengfei Cui³, Guohua Deng³, Dong Chu², Xuyong Li^1#

¹College of Agriculture and Biology, Liaocheng University, Liaocheng 252000, China
²Biological Disaster Control and Prevention Center, National Forestry and Grassland Administration, Shenyang 110034, China
³Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences/State Key Laboratory of Veterinary Biotechnology, National Poultry Laboratory Animal Resource Center, Harbin 150069, China

Received:2023-07-31 Revised:2024-01-03 Accepted:2023-11-24 Online:2025-06-20 Published:2025-05-13
About author:#Correspondence Xuyong Li, E-mail: lixuyong@lcu.edu.cn *These authors contributed equally to this study.
Supported by:
This work was supported by the National Key Research and Development Program of China (2021YFD1800200), the Natural Science Foundation of Shandong Province, China (ZR2021MC087), and the State Key Laboratory of Veterinary Biotechnology, China (SKLVBF202201).

摘要/Abstract

摘要：

野鸟中广泛流行的禽流感病毒极易通过野鸟-家禽或者鸟类-哺乳动物的交界面外溢至家禽或哺乳动物。H8N4亚型禽流感病毒属于稀有亚型，鲜有家禽感染H8N4禽流感病毒的报道。野生迁徙水禽是H8N4亚型禽流感病毒的主要储存库。目前，我们对该亚型病毒的生态学、流行病学以及生物学特性了解较少。本研究中，我们对H8N4亚型禽流感病毒的时空流行规律、宿主分布等进行了系统的统计和分析，详细绘制了病毒的遗传进化图谱。我们还报道了一株2022年在中国东部迁徙的白骨顶鸡分离的H8N4病毒，并对该病毒的生物学特性进行了系统研究。动物感染实验发现这株野鸟源的H8N4病毒可以在SPF家鸭体内有效复制和传播，但对SPF鸡的适应性较差。该野鸟源的H8N4病毒无需提前适应就可以在小鼠体内有效复制。这些结果表明，野鸭是H8N4亚型禽流感病毒的主要宿主，家鸭对该病毒的感染风险较高。因此，在野生水禽和家养水禽界面开展系统性的禽流感病毒监测将有助于及时发现这些病毒的流行和传播规律。

Abstract:

The circulating avian influenza viruses in wild birds have a high possibility of spillover into domestic birds or mammals at the wild bird–domestic bird or bird–mammal interface. H8N4 viruses primarily circulate in migratory wild waterfowl and have rarely been identified in domestic birds. In this study, we summarized the spatial and temporal distribution of global H8 viruses, specified their natural reservoirs, and performed detailed evolutionary analysis on the dominant H8N4 viruses. Here, we also report a novel H8N4 virus isolated from a Eurasian coot sample from a wetland in eastern China in 2022. Animal infection studies indicated that the wild bird-originated H8N4 virus can replicate and transmit efficiently in ducks but has not adapted to chickens. Additionally, this naturally isolated H8N4 virus can replicate in mice without prior adaptation. These results indicate that H8 viruses exist mainly in the wild duck reservoir and pose a high infection risk to domestic ducks. Therefore, the active surveillance of influenza viruses at the wild and domestic waterfowl interface will contribute to monitoring the circulation of these viruses.

Key words: avian influenza viruses , H8N4 , wild birds

Hong Zhang, Jing Guo, Peng Peng, Mengjing Wang, Jinyan Shen, Xiaohong Sun, Mengdi Guan, Pengfei Cui, Guohua Deng, Dong Chu, Xuyong Li. H8N4禽流感病毒的进化和生物学特性[J]. Journal of Integrative Agriculture, 2025, 24(6): 2342-2355.

Hong Zhang, Jing Guo, Peng Peng, Mengjing Wang, Jinyan Shen, Xiaohong Sun, Mengdi Guan, Pengfei Cui, Guohua Deng, Dong Chu, Xuyong Li. Evolution and biological characteristics of the circulated H8N4 avian influenza viruses[J]. Journal of Integrative Agriculture, 2025, 24(6): 2342-2355.

参考文献

Busquets N, Alba A, Napp S, Sanchez A, Serrano E, Rivas R, Nunez J I, Majo N. 2010. Influenza a virus subtypes in wild birds in North-Eastern Spain (Catalonia). Virus Research, 149, 10–18.

Chai H, Li X, Li M, Lv X, Yu W, Li Y, Sun J, Li Y, Sun H, Tian J, Xu Y, Bai X, Peng P, Xie L, Qin S, An Q, Zhang F, Zhang H, Du J, Yang S, et al. 2022. Emergence, evolution, and pathogenicity of influenza A (H7N4) virus in shorebirds in China. Journal of Virology, 96, e171721.

Chen Y, Bai T, Shu Y. 2022. Poultry to human passport: Cross-species transmission of zoonotic H7N9 avian influenza virus to humans. Zoonoses, 2, 4.

Cui P, Zeng X, Li X, Li Y, Shi J, Zhao C, Qu Z, Wang Y, Guo J, Gu W, Ma Q, Zhang Y, Lin W, Li M, Tian J, Wang D, Xing X, Liu Y, Pan S, Zhang Y, Bao H, Liu L, Tian G, Li C, et al. 2022. Genetic and biological characteristics of the globally circulating H5N8 avian influenza viruses and the protective efficacy offered by the poultry vaccine currently used in China. Science China Life Sciences, 65, 795–808.

Cui Y, Li Y, Li M, Zhao L, Wang D, Tian J, Bai X, Ci Y, Wu S, Wang F, Chen X, Ma S, Qu Z, Yang C, Liu L, Shi J, Guan Y, Zeng X, Tian G, Cui P, et al. 2020. Evolution and extensive reassortment of H5 influenza viruses isolated from wild birds in China over the past decade. Emerging Microbes & Infections, 9, 1793–1803.

Ferro P J, Khan O, Peterson M J, Batchuluun D, Reddy S M, Lupiani B. 2012. Avian influenza virus surveillance in hunter-harvested waterfowl, Texas coast, September 2009–January 2010. Avian Diseases, 56, 1006–1009.

Gass J D, Dusek R J, Hall J S, Hallgrimsson G T, Halldórsson H P, Vignisson S R, Ragnarsdottir S B, Jónsson J E, Krauss S, Wong S S, Wan X F, Akter S, Sreevatsan S, Trovão N S, Nutter F B, Runstadler J A, Hill N J. 2023. Global dissemination of influenza A virus is driven by wild bird migration through arctic and subarctic zones. Molecular Ecology, 32, 198–213.

Gunther A, Krone O, Svansson V, Pohlmann A, King J, Hallgrimsson G T, Skarpheethinsson K H, Sigurethardottir H, Jonsson S R, Beer M, Brugger B, Harder T. 2022. Iceland as stepping stone for spread of highly pathogenic avian influenza virus between Europe and North america. Emerging Infectious Diseases, 28, 2383–2388.

Guo J, Wang Y, Zhao C, Gao X, Zhang Y, Li J, Wang M, Zhang H, Liu W, Wang C, Xia Y, Xu L, He G, Shen J, Sun X, Wang W, Han X, Zhang X, Hou Z, Jin X, et al. 2021. Molecular characterization, receptor binding property, and replication in chickens and mice of H9N2 avian influenza viruses isolated from chickens, peafowls, and wild birds in eastern China. Emerging Microbes & Infections, 10, 2098–2112.

Huo X, Cui L B, Chen C, Wang D, Qi X, Zhou M H, Guo X, Wang F, Liu W J, Kong W, Ni D, Chi Y, Ge Y, Huang H, Hu F, Li C, Zhao X, Ren R, Bao C J, Gao G F, et al. 2018. Severe human infection with a novel avian-origin influenza A (H7N4) virus. Science Bulletin (Beijing), 63, 1043–1050.

Kandeil A, Patton C, Jones J C, Jeevan T, Harrington W N, Trifkovic S, Seiler J P, Fabrizio T, Woodard K, Turner J C, Crumpton J C, Miller L, Rubrum A, Debeauchamp J, Russell C J, Govorkova E A, Vogel P, Kim-Torchetti M, Berhane Y, Stallknecht D, et al. 2023. Rapid evolution of A (H5N1) influenza viruses after intercontinental spread to North America. Nature Communications, 14, 3082.

Li C, Chen H. 2021. H7N9 influenza virus in China. Cold Spring Harbor Perspectives in Medicine, 11, a38349.

Li J, Zhang C, Cao J, Yang Y, Dong H, Cui Y, Yao X, Zhou H, Lu L, Lycett S, Wang X, Song H, Liu W, Gao G F, Shi W, Bi Y. 2021. Re-emergence of H5N8 highly pathogenic avian influenza virus in wild birds, China. Emerg Microbes Infect, 10, 1819–1823.

Muzyka D, Pantin-Jackwood M, Spackman E, Smith D, Rula O, Muzyka N, Stegniy B. 2016. Isolation and genetic characterization of avian influenza viruses isolated from wild birds in the azov-black sea region of ukraine (2001–2012). Avian Diseases, 60, 365–377.

Prosser D J, Chen J, Ahlstrom C A, Reeves A B, Poulson R L, Sullivan J D, Mcauley D, Callahan C R, Mcgowan P C, Bahl J, Stallknecht D E, Ramey A M. 2022. Maintenance and dissemination of avian-origin influenza a virus within the northern Atlantic Flyway of North America. PLoS Pathogens, 18, e1010605.

Qi X, Qiu H, Hao S, Zhu F, Huang Y, Xu K, Yu H, Wang D, Zhou L, Dai Q, Zhou Y, Wang S, Huang H, Yu S, Huo X, Chen K, Liu J, Hu J, Wu M, Bao C. 2022. Human infection with an avian-origin influenza A (H10N3) virus. The New England Journal of Medicine, 386, 1087–1088.

Shi J, Zeng X, Cui P, Yan C, Chen H. 2023. Alarming situation of emerging H5 and H7 avian influenza and effective control strategies. Emerging Microbes & Infections, 12, 2155072.

Tang W, Li X, Hu C H, Zhu C, Li Z, Wu D, Wang T, He G. 2019. Isolation of H8N4 avian influenza virus from wild birds in Shanghai, China. Acta Virologica, 63, 121–125.

Tian J, Bai X, Li M, Zeng X, Xu J, Li P, Wang M, Song X, Zhao Z, Tian G, Liu L, Guan Y, Li Y, Chen H. 2023. Highly pathogenic avian influenza virus (H5N1) Clade 2.3.4.4b introduced by wild birds, China, 2021. Emerging Infectious Diseases, 29, 1367–1375.

Veits J, Weber S, Stech O, Breithaupt A, Graber M, Gohrbandt S, Bogs J, Hundt J, Teifke J P, Mettenleiter T C, Stech J. 2012. Avian influenza virus hemagglutinins H2, H4, H8, and H14 support a highly pathogenic phenotype. Proceedings of the National Academy of Sciences of the United States of America, 109, 2579–2584.

Verhagen J H, Eriksson P, Leijten L, Blixt O, Olsen B, Waldenstrom J, Ellstrom P, Kuiken T. 2021. Host range of influenza a virus H1 to H16 in Eurasian ducks based on tissue and receptor binding studies. Journal of Virology, 95, e1820–e1873.

Wang D, Li M, Xiong C, Yan Y, Hu J, Hao M, Liang B, Chen J, Chen G, Yang G, Li Y, Zhang J, Gulyaeva M, Shestopalov A, Shi W, Bi Y, Liu H, Wang H, Liu D, Chen J. 2021. Ecology of avian influenza viruses in migratory birds wintering within the Yangtze River wetlands. Science Bulletin (Beijing), 66, 2014–2024.

Wang S, Wang D. 2023. Co-circulation, co-infection of SARS-CoV–2 and influenza virus, where will it go? Zoonoses, 3, 20.

Wang Y, Wang M, Zhang H, Zhao C, Zhang Y, He G, Deng G, Cui P, Li Y, Liu W, Shen J, Sun X, Wang W, Zeng X, Li Y, Chu D, Peng P, Guo J, Chen H, Li X. 2022a. Emergence, evolution, and biological characteristics of H10N4 and H10N8 avian influenza viruses in migratory wild birds detected in eastern China in 2020. Microbiology spectrum, 10, e80722.

Wang Y, Wang M, Zhang H, Zhao C, Zhang Y, Shen J, Sun X, Xu H, Xie Y, Gao X, Cui P, Chu D, Li Y, Liu W, Peng P, Deng G, Guo J, Li X. 2023. Prevalence, evolution, replication and transmission of H3N8 avian influenza viruses isolated from migratory birds in eastern China from 2017 to 2021. Emerging Microbes & Infections, 12, 2184178.

Wang Y, Zhang H, Wang M, Guo J, Zhao C, Cui P, Deng G, Chu D, Shen J, Sun X, Gao X, Li Y, Liu W, Peng P, Li X. 2022b. Genetic analysis of a novel H16N3 virus isolated from a migratory gull in China in 2021 and animal studies of infection. Microbiology Spectrum, 10, e248422.

Wille M, Grillo V, de Gouvea Pedroso S B, Burgess G W, Crawley A, Dickason C, Hansbro P M, Hoque M A, Horwood P F, Kirkland P D, Kung N Y, Lynch S E, Martin S, Mcarthur M, O’Riley K, Read A J, Warner S, Hoye B J, Lisovski S, Leen T, et al. 2022. Australia as a global sink for the genetic diversity of avian influenza a virus. PLoS Pathogens, 18, e1010150.

Xu Q, Lv X, Li Y, Luo H, Zhang Z, Li X, Qin R, An Q, Qu F, Zhao Z, Zhang C, Wang W, Li Y, Wang Y, Zeng X, Hou Z, Ren J, Wang Y, Li Y, Chai H. 2023. Emergence of highly pathogenic avian influenza a (H5N8) clade 2.3.4.4b viruses in grebes in Inner Mongolia and Ningxia, China, 2021. Journal of Integrative Agriculture, 23, 348–353.

Yang H, Carney P J, Chang J C, Stevens J. 2020. Molecular characterization and three-dimensional structures of avian H8, H11, H14, H15 and swine H4 influenza virus hemagglutinins. Heliyon, 6, e4068.

Yang J, Zhang Y, Yang L, Li X, Bo H, Liu J, Tan M, Zhu W, Shu Y, Wang D. 2023. Evolution of avian influenza virus (H3) with spillover into humans, China. Emerging Infectious Diseases, 29, 1191–1201.

Zeng X, Chen X, Ma S, Wu J, Bao H, Pan S, Liu Y, Deng G, Shi J, Chen P, Jiang Y, Li Y, Hu J, Lu T, Mao S, Guo X, Liu J, Tian G, Chen H. 2020. Protective efficacy of an H5/H7 trivalent inactivated vaccine produced from Re-11, Re-12, and H7-Re2 strains against challenge with different H5 and H7 viruses in chickens. Journal of Integrative Agriculture, 19, 2294–2300.

Zeng X, He X, Meng F, Ma Q, Wang Y, Bao H, Liu Y, Deng G, Shi J, Li Y, Tian G, Chen H. 2022. Protective efficacy of an H5/H7 trivalent inactivated vaccine (H5-Re13, H5-Re14, and H7-Re4 strains) in chickens, ducks, and geese against newly detected H5N1, H5N6, H5N8, and H7N9 viruses. Journal of Integrative Agriculture, 21, 2086–2094.

Zhang G, Li B, Raghwani J, Vrancken B, Jia R, Hill S C, Fournie G, Cheng Y, Yang Q, Wang Y, Wang Z, Dong L, Pybus O G, Tian H. 2023. Bidirectional movement of emerging H5N8 avian influenza viruses between europe and Asia via migratory birds since early 2020. Molecular Biology and Evolution, 40, d19.

Zhao C, Guo J, Zeng X, Shi J, Deng G, Zhang Y, Wang Y, Ma Q, Gao X, Cui P, Liu L, Li X, Chen H. 2022. Novel H7N7 avian influenza viruses detected in migratory wild birds in eastern China between 2018 and 2020. Microbes and Infection, 24, 105013.

H8N4禽流感病毒的进化和生物学特性

Evolution and biological characteristics of the circulated H8N4 avian influenza viruses

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