PTP1B和PTK抑制剂对亚洲小车蝗发育的协同调节作用

doi:10.1016/j.jia.2023.12.020

Journal of Integrative Agriculture ›› 2024, Vol. 23 ›› Issue (8): 2752-2763.DOI: 10.1016/j.jia.2023.12.020

PTP1B和PTK抑制剂对亚洲小车蝗发育的协同调节作用

收稿日期:2023-07-14 接受日期:2023-11-02 出版日期:2024-08-20 发布日期:2024-07-29

The synergistic regulatory effect of PTP1B and PTK inhibitors on the development of Oedaleus decorus asiaticus Bei-Bienko

Shuang Li¹^,²^{, 5*}, Sibo Liu^{3, 4}^*, Chaomin Xu^{1, 5}, Shiqian Feng^1,⁵, Xiongbing Tu^1,^5#, Zehua Zhang^1,⁵

¹ State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
² Chongqing Academy of Agricultural Sciences, Chongqing 401329, China
³ Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot 010010, China
⁴ Inner Mongolia University, Hohhot 010019, China
⁵ Scientific Observation and Experimental Station of Pests in Xilin Gol Rangeland, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Xilinhot 026000, China

Received:2023-07-14 Accepted:2023-11-02 Online:2024-08-20 Published:2024-07-29
About author:Shuang Li, E-mail: sclishuang61@163.com; Sibo Liu, E-mail: lsbss258@126.com; #Correspondence Xiongbing Tu, E-mail: xbtu@ippcaas.cn * These authors contributed equally to this study.
Supported by:
This research was funded by the National Key R&D Program of China (2022YFD1400500), the earmarked fund for China Agriculture Research System (CARS-34) and the Central Public-interest Scientific Institution Basal Research Fund of China (S2022XM21).

摘要/Abstract

摘要：

酪氨酸磷酸化对控制正常细胞生长、存活、胞间交流、基因转录、免疫反应和其他过程至关重要。PTP（蛋白酪氨酸磷酸酶）和PTK（蛋白酪氨酸激酶）可通过调节多种信号通路来实现这一目标。亚洲小车蝗是蒙古高原草原上的一种重要害虫。我们的目的是在分别抑制PTP1B（蛋白酪氨酸磷酸酶-1B）和PTK活性的同时，评估亚洲小车蝗4龄若虫的存活率、生长速率、总体表现力和雌虫卵巢发育形态。此外，还评估了两种处理下亚洲小车蝗MAPK信号通路中关键基因表达、蛋白磷酸化水平以及抗氧化酶活性。结果发现，PTP1B抑制剂处理后的4龄若虫和对照组在存活率、生长率或总体表现方面无显著差异，而PTK抑制剂处理后，以上指标显著低于对照组。用PTK抑制剂处理4龄若虫15天后，雌虫的卵巢大小及发育显著小于对照，而PTP1B抑制剂处理结果与之相反。与对照和PTK抑制剂处理的若虫相比，4龄若虫在PTP1B抑制剂处理下的MAPK信号通路中关键基因的表达和磷酸化水平显著较高。而ROS水平、NADPH氧化酶和其他抗氧化酶的活性显著降低，此结果与PTK抑制剂相反。因此，PTP1B和PTK在MAPK信号传递中的反馈抑制可以调节蝗虫的生理代谢和发育速率。这些发现促进了将PTP1B和PTK抑制剂用于调控昆虫发育速率，以帮助控制田间害虫种群。

Abstract:

Tyrosine phosphorylation is crucial for controlling normal cell growth, survival, intercellular communication, gene transcription, immune responses, and other processes. protein tyrosine phosphatase (PTP) and protein tyrosine kinases (PTK) can achieve this goal by regulating multiple signaling pathways. Oedaleus decorus asiaticus is an important pest that infests the Mongolian Plateau grassland. We aimed to evaluate the survival rate, growth rate, overall performance, and ovarian developmental morphology of the 4th instar nymphs of O. decorus asiaticus while inhibiting the activity of protein tyrosine phosphatase-1B (PTP1B) and PTK. In addition, the expression and protein phosphorylation levels of key genes in the MAPK signaling pathway and antioxidant enzyme activity were assessed. The results showed no significant differences in survival rate, growth rate, or overall performance between PTP1B inhibitor treatment and control. However, after PTK inhibitor treatment, these indexes were significantly lower than those in the control. The ovarian size of female larvae after 15 days of treatment with PTK inhibitors showed significantly slower development, while female larvae treated with PTP1B exhibited faster ovarian growth than the control group. In comparison to controls and nymphs treated with PTK inhibitors, the expression and phosphorylation levels of key genes in the MAPK signaling pathway under PTP1B inhibitor treatments were significantly higher in 4th instar nymphs. However, reactiveoxygen (ROS) species levels and the activities of NADPH oxidase and other antioxidant enzymes were considerably reduced, although they were significantly greater in the PTK inhibitor treatment. The results suggest that PTP1B and PTK feedback inhibition in the mitogen-activated-protein kinases (MAPK) signal transfer can regulate the physiological metabolism of the insect as well as its developmental rate. These findings can facilitate future uses of PTP1B and PTK inhibitors in controlling insect development to help control pest populations.

Key words: PTP1B , PTK , inhibito , MAPK pathway , Oedaleus decorus asiaticus , development

Shuang Li, Sibo Liu, Chaomin Xu, Shiqian Feng, Xiongbing Tu, Zehua Zhang. PTP1B和PTK抑制剂对亚洲小车蝗发育的协同调节作用[J]. Journal of Integrative Agriculture, 2024, 23(8): 2752-2763.

Shuang Li, Sibo Liu, Chaomin Xu, Shiqian Feng, Xiongbing Tu, Zehua Zhang. The synergistic regulatory effect of PTP1B and PTK inhibitors on the development of Oedaleus decorus asiaticus Bei-Bienko[J]. Journal of Integrative Agriculture, 2024, 23(8): 2752-2763.

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PTP1B和PTK抑制剂对亚洲小车蝗发育的协同调节作用

The synergistic regulatory effect of PTP1B and PTK inhibitors on the development of Oedaleus decorus asiaticus Bei-Bienko

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