TaFLZ54D通过与TaSGT1a和TaPP2Ca互作提高小麦的耐盐性

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (3): 1017-1029.DOI: 10.1016/j.jia.2023.09.018

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TaFLZ54D通过与TaSGT1a和TaPP2Ca互作提高小麦的耐盐性

收稿日期:2023-05-25 接受日期:2023-08-09 出版日期:2025-03-20 发布日期:2025-02-28

TaFLZ54D enhances salt stress tolerance in wheat by interacting with TaSGT1 and TaPP2C

Yuxiang Qin^1#, Bao Zhang¹, Shoufu Cui¹, Xiaochun Qin¹, Genying Li²

¹School of Biological Science and Technology, University of Jinan, Jinan 250022, China
²Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China

Received:2023-05-25 Accepted:2023-08-09 Online:2025-03-20 Published:2025-02-28
About author:#Correspondence Yuxiang Qin, Mobile: +86-13065092739, E-mail: chm_qinyx@ujn.edu.cn
Supported by:
The study was supported by the National Natural Science Foundation of China (31871622) and the Key R&D Program of Shandong Province, China (2022LZG001).

摘要/Abstract

摘要：

FCS-like zinc finger (FLZ)是C2-C2类锌指蛋白基因家族，参与种子的休眠，对蚜虫的抗性，糖信号和非生物胁迫响应。但是，人们对FLZs基因家族的功能，特别是其作用的分子机制的了解还非常有限。本研究，我们在小麦中鉴定了120个FLZ基因家族成员，利用转基因小麦揭示了TaFLZ54D的耐盐功能及其作用机制。表达研究表明TaFLZ54D受NaCl诱导表达，且在120个FLZs中表达量最高。超表达TaFLZ54D可以提高小麦的耐盐性。转基因小麦植株具有较高的SOD、POD酶活性、较高的可溶性糖含量和较低的Na⁺/K⁺比及MDA含量。钾离子跨膜转运蛋白和丝氨酸/苏氨酸激酶抑制因子在转基因植株和野生型植株中差异表达。酵母双杂交和荧光素酶互补实验分析表明TaSGT1a和TaPP2Ca是TsFLZ54D的直接相互作用蛋白。总之，TaFLZ54D通过与TaSGT1a和TaPP2Ca直接互作，降低Na⁺的吸收和减少氧化胁迫提高了耐盐性。TaFLZ54D与TaSGT1a和TaPP2Ca之间的互作表明：TaFLZ54D的耐盐性可能与负调控蛋白的泛素化降解之间存在一定关系。

Abstract:

FCS-like zinc finger (FLZ) gene family members are C2-C2 zinc finger proteins that take part in seed dormancy, resistance to Myzus persicae 1, sucrose signaling and abiotic stresse tolerance. However, their functions, especially the molecular mechanism through which FLZs function, are not well understood. In this study, we characterized 120 FLZs in wheat and revealed the function and mechanism of TaFLZ54D increasing salt stress tolerance in transgenic wheat. Expression analysis demonstrated that TaFLZ54D can be induced by NaCl treatment and it had the highest expression level under NaCl treatment among the 120 FLZs. Over-expression of TaFLZ54D increased wheat salt stress tolerance and the transgenic plants had higher levels of superoxide dismutase (SOD) and peroxidase (POD) activities and soluble sugar content, but a lower Na⁺/K⁺ ratio and malondialdehyde (MDA) content than the wild type (WT) plants. Potassium ion transmembrane transporters and serine/threonine kinase inhibitor proteins showed differential expression between TaFLZ54D transgenic wheat and the WT. Yeast two hybrid and luciferase complementation assays revealed that TaSGT1 and TaPP2C are the proteins that interact directly with TaFLZ54D. In summary, TaFLZ54D enhances salt stress tolerance through interaction with TaSGT1 and TaPP2C to reduce Na⁺absorption and mitigate oxidative stress. The interaction between TaFLZ54D and TaSGT1, as well as TaPP2C indicated a link between salt stress tolerance of TaFLZ54D and the ubiquitin-mediated degradation of negative regulatory proteins.

Key words: wheat , FLZ , salt tolerance , protein interaction , Na⁺ exclusion

Yuxiang Qin, Bao Zhang, Shoufu Cui, Xiaochun Qin, Genying Li. TaFLZ54D通过与TaSGT1a和TaPP2Ca互作提高小麦的耐盐性[J]. Journal of Integrative Agriculture, 2025, 24(3): 1017-1029.

Yuxiang Qin, Bao Zhang, Shoufu Cui, Xiaochun Qin, Genying Li. TaFLZ54D enhances salt stress tolerance in wheat by interacting with TaSGT1 and TaPP2C[J]. Journal of Integrative Agriculture, 2025, 24(3): 1017-1029.

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