籽粒蛋白质含量相关的4个QTLs在现代小麦品种中的选择与应用

doi:10.1016/j.jia.2023.09.006

摘要/Abstract

摘要：

籽粒蛋白质含量（GPC）是影响小麦籽粒营养成分的关键因素。本研究对406份小麦材料在8种环境中的GPC进行了重采样的全基因组关联分析（GWAS），鉴定到4个与GPC相关且被多次报道过的QTLs。通过对87个地方小麦和259个现代栽培品种的分析表明，这4个与GPC相关QTLs的优势单倍型在中国现代栽培品种中正逐渐缺失。但这些单倍型被中国不同小麦生产区采用后会对小麦产量及相关农艺性状产生重要影响。且其中大多数与GPC相关的QTLs对小麦产量没有显著的负面效应，表明可以利用它们在保持产量的同时提高小麦籽粒蛋白质含量。该研究可为今后利用这4个QTLs进行小麦籽粒蛋白质含量育种提供重要参考。

Abstract:

The grain protein content (GPC) is the key parameter for wheat grain nutritional quality. This study conducted a resampling GWAS analysis using 406 wheat accessions across eight environments, and identified four previously reported GPC QTLs. An analysis of 87 landraces and 259 modern cultivars revealed the loss of superior GPC haplotypes, especially in Chinese cultivars. These haplotypes were preferentially adopted in different agroecological zones and had broad effects on wheat yield and agronomic traits. Most GPC QTLs did not significantly reduce yield, suggesting that high GPC can be achieved without a yield penalty. The results of this study provide a reference for future GPC breeding in wheat using the four identified QTLs.

Key words: breeding , grain protein content , haplotype selection and application , wheat

Zihui Liu, Xiangjun Lai, Yijin Chen, Peng Zhao, Xiaoming Wang, Wanquan Ji, Shengbao Xu. 籽粒蛋白质含量相关的4个QTLs在现代小麦品种中的选择与应用[J]. Journal of Integrative Agriculture, 2024, 23(8): 2557-2570.

Zihui Liu, Xiangjun Lai, Yijin Chen, Peng Zhao, Xiaoming Wang, Wanquan Ji, Shengbao Xu. Selection and application of four QTLs for grain protein content in modern wheat cultivars[J]. Journal of Integrative Agriculture, 2024, 23(8): 2557-2570.

参考文献

Alvarez J B, Guzman C. 2018. Interspecific and intergeneric hybridization as a source of variation for wheat grain quality improvement. Theoretical and Applied Genetics, 131, 225–251.

Bajgain P, Sallam A, Annor G, Conley E, Steffenson B, Muehlbauer G, Anderson J A. 2020. Genetic characterization of flour quality and bread-making traits in a spring wheat nested association mapping population. Crop Science, 61, 1168–1183.

Balyan H S, Gupta P K, Kumar S, Dhariwal R, Jaiswal V, Tyagi S, Agarwal P, Gahlaut V, Kumari S. 2013. Genetic improvement of grain protein content and other health-related constituents of wheat grain. Plant Breeding, 132, 446–457.

Battenfield S D, Guzmán C, Gaynor R C, Singh R P, Peña R J, Dreisigacker S, Fritz A K, Poland J A. 2016. Genomic selection for processing and end-use quality traits in the CIMMYT spring bread wheat breeding program. The Plant Genome, 9, 1–12.

Blanco A, Mangini G, Giancaspro A, Giove S, Colasuonno P, Simeone R, Signorile A, De Vita P, Mastrangelo A M, Cattivelli L, Gadaleta A. 2012. Relationships between grain protein content and grain yield components through quantitative trait locus analyses in a recombinant inbred line population derived from two elite durum wheat cultivars. Molecular Breeding, 30, 79–92.

Bogard M, Allard V, Martre P, Heumez E, Snape J W, Orford S, Griffiths S, Gaju O, Foulkes J, Le Gouis J. 2013. Identifying wheat genomic regions for improving grain protein concentration independently of grain yield using multiple inter-related populations. Molecular Breeding, 31, 587–599.

Chen J, Zhang F, Zhao C, Lv G, Sun C, Pan Y, Guo X, Chen F. 2019. Genome-wide association study of six quality traits reveals the association of the TaRPP13L1 gene with flour colour in Chinese bread wheat. Plant Biotechnol Journal, 17, 2106–2122.

Chen Q, Guo Z, Shi X, Wei M, Fan Y, Zhu J, Zheng T, Wang Y, Kong L, Deng M, Cao X, Wang J, Wei Y, Jiang Q, Jiang Y, Chen G, Zheng Y, Qi P. 2022. Increasing the grain yield and yrain protein content of common wheat (Triticum aestivum) by introducing missense mutations in the Q gene. International Journal of Molecular Sciences, 23, 10772.

Chu J, Guo X, Zheng F, Zhang X, Dai X, He M. 2023. Effect of delayed sowing on grain number, grain weight, and protein concentration of wheat grains at specific positions within spikes. Journal of Integrative Agriculture, 22, 2359–2369.

Danecek P, Auton A, Abecasis G, Albers C A, Banks E, DePristo M A, Handsaker R E, Lunter G, Marth G T, Sherry S T, McVean G, Durbin R. 2011. The variant call format and VCFtools. Bioinformatics, 27, 2156–2158.

Distelfeld A, Uauy C, Fahima T, Dubcovsky J. 2006. Physical map of the wheat high-grain protein content gene Gpc-B1 and development of a high-throughput molecular marker. New Phytologist, 169, 753–763.

Dong S, Zhang X, Chu J, Zheng F, Fei L, Dai X, He M. 2022. Optimized seeding rate and nitrogen topdressing ratio for simultaneous improvement of grain yield and bread-making quality in bread wheat sown on different dates. Journal of the Science of Food and Agriculture, 102, 360–369.

Du X, Ma X, Min J, Zhang X, Jia Z. 2018. Development of a wheat–Aegilops searsii substitution line with positively affecting Chinese steamed bread quality. Breeding Science, 68, 289–293.

Fan X, Liu X, Feng B, Zhou Q, Deng G, Long H, Cao J, Guo S, Ji G, Xu Z, Wang T. 2022. Construction of a novel Wheat 55 K SNP array-derived genetic map and its utilization in QTL mapping for grain yield and quality related traits. Frontiers in Genetics, 13, 978880.

Geyer M, Mohler V, Hartl L. 2022. Genetics of the inverse relationship between grain yield and grain protein content in common wheat. Plants (Basel, Switzerland), 11, 2146.

Gudi S, Saini D K, Singh G, Halladakeri P, Kumar P, Shamshad M, Tanin M J, Singh S, Sharma A. 2022. Unravelling consensus genomic regions associated with quality traits in wheat using meta-analysis of quantitative trait loci. Planta, 255, 115.

Guo J, Li C, Zhao J, Guo J, Shi W, Cheng S, Zhou M, Hao C. 2020. Ecological genomics of Chinese wheat improvement: implications in breeding for adaptation. BMC Plant Biology, 20, 494.

Jin X, Feng B, Xu Z, Fan X, Liu J, Liu Q, Zhu P, Wang T. 2018. TaAAP6-3B, a regulator of grain protein content selected during wheat improvement. BMC Plant Biology, 18, 71.

Kunert A, Naz A A, Dedeck O, Pillen K, Léon J. 2007. AB-QTL analysis in winter wheat: I. Synthetic hexaploid wheat (T. turgidum ssp. dicoccoides×T. tauschii) as a source of favourable alleles for milling and baking quality traits. Theoretical and Applied Genetics, 115, 683–695.

Laidig F, Piepho H P, Rentel D, Drobek T, Meyer U, Huesken A. 2017. Breeding progress, environmental variation and correlation of winter wheat yield and quality traits in German official variety trials and on-farm during 1983–2014. Theoretical and Applied Genetics, 130, 223–245.

Liu Z, Hu Z, Zhu Z, Cao J, Zhang J, Ma X, Zhang X, Wang X, Ji W, Xu S. 2022. Multi-environmental population phenotyping suggest the higher risks of wheat Rht-B1b and Rht-D1b cultivars in global warming scenarios. BioRxiv, doi: 2022.07.18.500398.

Lou H, Zhang R, Liu Y, Guo D, Zhai S, Chen A, Zhang Y, Xie C, You M, Peng H, Liang R, Ni Z, Sun Q, Li B. 2021. Genome-wide association study of six quality-related traits in common wheat (Triticum aestivum L.) under two sowing conditions. Theoretical and Applied Genetics, 134, 399–418.

Ma J, Xiao Y, Hou L, He Y. 2021. Combining protein content and grain yield by genetic dissection in bread wheat under low-input management. Foods (Basel, Switzerland), 10, 1058.

Mefleh M, Conte P, Fadda C, Giunta F, Motzo R. 2020. From seed to bread: Variation in quality in a set of old durum wheat cultivars. Journal of the Science of Food and Agriculture, 100, 4066–4074.

Mesfin A, Frohberg R C, Khan K, Olson T C. 2000. Increased grain protein content and its association with agronomic and end-use quality in two hard red spring wheat populations derived from Triticum turgidum L. var. dicoccoides. Euphytica, 116, 237–242.

Michel S, Loschenberger F, Ametz C, Pachler B, Sparry E, Burstmayr H. 2019. Combining grain yield, protein content and protein quality by multi-trait genomic selection in bread wheat. Theoretical and Applied Genetics, 132, 2767–2780.

Nigro D, Gadaleta A, Mangini G, Colasuonno P, Marcotuli I, Giancaspro A, Giove S L, Simeone R, Blanco A. 2019. Candidate genes and genome-wide association study of grain protein content and protein deviation in durum wheat. Planta, 249, 1157–1175.

Prasad M, Kumar N, Kulwal P, Röder M, Balyan H, Dhaliwal H, Gupta P. 2003. QTL analysis for grain protein content using SSR markers and validation studies using NILs in bread wheat. Theoretical and Applied Genetics, 106, 659–667.

Pu Z, Ye X, Li Y, Shi B, Guo Z, Dai S, Ma J, Liu Z, Jiang Y, Li W, Jiang Q, Chen G, Wei Y, Zheng Y. 2022. Identification and validation of novel loci associated with wheat quality through a genome-wide association study. Journal of Integrative Agriculture, 21, 3131–3147.

Rapp M, Lein V, Lacoudre F, Lafferty J, Müller E, Vida G, Bozhanova V, Ibraliu A, Thorwarth P, Piepho H P, Leiser W L, Würschum T, Longin C F H. 2018. Simultaneous improvement of grain yield and protein content in durum wheat by different phenotypic indices and genomic selection. Theoretical and Applied Genetics, 131, 1315–1329.

Rharrabti Y, Elhani S, Martos-Núñez V, García Del Moral L F. 2001. Protein and lysine content, grain yield, and other technological traits in durum wheat under Mediterranean conditions. Journal of Agricultural and Food Chemistry, 49, 3802–3807.

Saini P, Sheikh I, Saini D K, Mir R R, Dhaliwal H S, Tyagi V. 2022. Consensus genomic regions associated with grain protein content in hexaploid and tetraploid wheat. Frontiers in Genetics, 13, 1021180.

Sallam A H, Manan F, Bajgain P, Martin M, Szinyei T, Conley E, Brown-Guedira G, Muehlbauer G J, Anderson J A, Steffenson B J. 2020. Genetic architecture of agronomic and quality traits in a nested association mapping population of spring wheat. The Plant Genome, 13, e20051.

Semagn K, Iqbal M, Chen H, Perez-Lara E, Bemister D H, Xiang R, Zou J, Asif M, Kamran A, N’Diaye A, Randhawa H, Beres B L, Pozniak C, Spaner D. 2021. Physical mapping of QTL associated with agronomic and end-use quality traits in spring wheat under conventional and organic management systems. Theoretical and Applied Genetics, 134, 3699–3719.

Shewry P R. 2009. Wheat. Journal of Experimental Botany, 60, 1537–1553.

Shewry P R, Hey S J. 2015. The contribution of wheat to human diet and health. Food Energy Secur, 4, 178–202.

Soriano J M, Colasuonno P, Marcotuli I, Gadaleta A. 2021. Meta-QTL analysis and identification of candidate genes for quality, abiotic and biotic stress in durum wheat. Scientific Reports, 11, 11877.

Thorwarth P, Liu G, Ebmeyer E, Schacht J, Schachschneider R, Kazman E, Reif J C, Wurschum T, Longin C F H. 2019. Dissecting the genetics underlying the relationship between protein content and grain yield in a large hybrid wheat population. Theoretical and Applied Genetics, 132, 489–500.

Tian F, Bradbury P J, Brown P J, Hung H, Sun Q, Flint-Garcia S, Rocheford T R, McMullen M D, Holland J B, Buckler E S. 2011. Genome-wide association study of leaf architecture in the maize nested association mapping population. Nature Genetics, 43, 159–162.

Uauy C, Brevis J C, Dubcovsky J. 2006. The high grain protein content gene Gpc-B1 accelerates senescence and has pleiotropic effects on protein content in wheat. Journal of Experimental Botany, 57, 2785–2794.

Valdar W, Holmes C C, Mott R, Flint J. 2009. Mapping in structured populations by resample model averaging. Genetics, 182, 1263–1277.

Vilella A J, Blanco-Garcia A, Hutter S, Rozas J. 2005. VariScan: Analysis of evolutionary patterns from large-scale DNA sequence polymorphism data. Bioinformatics (Oxford, England), 21, 2791–2793.

Wang X, Zhao P, Guo X, Liu Z, Ma X, Zhao Y, Lai X, Huang L, Wang W, Han D, Kang Z, Xu S. 2022. Population transcriptome and phenotype reveal that the Rht-D1b contribute a larger seedling roots to modern wheat cultivars. BioRxiv, doi: 2022.06.02.494553.

Xiao J, Liu B, Yao Y, Guo Z, Jia H, Kong L, Zhang A, Ma W, Ni Z, Xu S, Lu F, Jiao Y, Yang W, Lin X, Sun S, Lu Z, Gao L, Zhao G, Cao S, Chen Q, et al. 2022. Wheat genomic study for genetic improvement of traits in China. Science China (Life Sciences), 65, 1718–1775.

Yang Y, Chai Y, Zhang X, Lu S, Zhao Z, Wei D, Chen L, Hu Y G. 2020. Multi-locus GWAS of quality traits in bread wheat: mining more candidate genes and possible regulatory network. Frontiers in Plant Science, 11, 1091.

Zhao L, Zhang K P, Liu B, Deng Z Y, Qu H L, Tian J C. 2010. A comparison of grain protein content QTLs and flour protein content QTLs across environments in cultivated wheat. Euphytica, 174, 325–335.