饲粮添加锰对肉鸡腹脂沉积的影响

doi:10.1016/j.jia.2023.08.004

Journal of Integrative Agriculture ›› 2024, Vol. 23 ›› Issue (12): 4161-4171.DOI: 10.1016/j.jia.2023.08.004

饲粮添加锰对肉鸡腹脂沉积的影响

收稿日期:2023-04-11 接受日期:2023-06-28 出版日期:2024-12-20 发布日期:2024-11-18

Dietary manganese supplementation inhibits abdominal fat deposition possibly by regulating gene expression and enzyme activity involved in lipid metabolism in the abdominal fat of broilers

Xiaoyan Cui¹, Ke Yang^1,^2,³, Weiyun Zhang¹, Liyang Zhang⁴, Ding Li¹, Wei Wu¹, Yun Hu¹, Tingting Li¹, Xugang Luo^1#

¹Poultry Mineral Nutrition Laboratory, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
²Department of Animal Science, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China
³Hebei Provincial Key Laboratory of Characteristic Animal Germplasm Resources Mining and Innovation, Qinhuangdao 066004, China
⁴Mineral Nutrition Research Division, State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received:2023-04-11 Accepted:2023-06-28 Online:2024-12-20 Published:2024-11-18
About author:Xiaoyan Cui, E-mail: xycui@yzu.edu.cn; #Correspondence Xugang Luo, Tel: +86-514-87976732, E-mail: wlysz@263.net
Supported by:

This work was financially supported by the National Natural Science Foundation of China (32102559), the Jiangsu Shuang Chuang Tuan Dui Program, China (JSSCTD202147), and the Jiangsu Shuang Chuang Ren Cai Program, China (JSSCRC2021541).

摘要/Abstract

摘要：

腹脂沉积过多严重制约了肉鸡的生产效率。研究发现，饲粮添加锰可有效降低肉鸡腹脂沉积，但其潜在机制尚不清楚。本试验旨在研究饲粮添加无机或有机锰对公母鸡腹脂沉积、脂肪代谢相关酶活及基因表达的影响。试验选取1日龄AA肉公鸡和AA肉母鸡各210只，采用2（性别）× 3（饲粮锰处理）双因子完全随机试验设计，共6个试验处理组，每个处理10个重复，每个重复7只鸡。公鸡和母鸡饲喂含有17.52 mg/kg锰（1-21天）和15.62 mg/kg锰（22-42天）的不添加锰的基础饲粮，或添加110 mg/kg锰（1-21天）和80 mg/kg锰（22-42天）的硫酸锰或中等螯合强度蛋白锰，试验周期42天。结果显示，饲粮锰添加与性别对所有指标均无显著交互作用（P>0.05）；锰的添加使肉鸡腹脂率降低（P<0.003）；与对照组相比，锰添加增加了（P<0.004）肉鸡腹脂中的脂肪甘油三酯脂酶（ATGL）活性，而中等螯合强度蛋白锰降低了肉鸡腹脂中（P>0.002）脂肪酸合成酶（FAS）活性；锰添加降低了（P<0.009）肉鸡腹脂中二酰基甘油酰基转移酶2（DGAT2）mRNA的表达水平和过氧化物酶体增殖物激活受体γ（PPARγ）mRNA和蛋白的表达水平，但上调了肉鸡腹脂中（P＞0.05）ATGL的mRNA和蛋白表达水平。结果表明，饲粮添加锰可能通过降低肉鸡腹脂中PPARγ和DGAT2的表达以及增加肉鸡腹脂中ATGL的表达和活性来抑制肉鸡腹脂沉积，而中等螯合强度蛋白锰对FAS活性的抑制作用更为明显。

Abstract:

Excessive abdominal fat deposition seriously restricts the production efficiency of broilers. Several studies found that dietary supplemental manganese (Mn) could effectively reduce the abdominal fat deposition of broilers, but the underlying mechanisms remain unclear. The present study aimed to investigate the effect of dietary supplementation with the inorganic or organic Mn on abdominal fat deposition, and enzyme activity and gene expression involved in lipid metabolism in the abdominal fat of male or female broilers. A total of 420 1-d-old AA broilers (half males and half females) were randomly allotted by body weight and gender to 1 of 6 treatments with 10 replicates cages of 7 chicks per cage in a completely randomized design involving a 3 (dietary Mn addition)×2 (gender) factorial arrangement. Male or female broilers were fed with the Mn-unsupplemented basal diets containing 17.52 mg Mn kg^–1 (d 1–21) and 15.62 mg Mn kg^–1 (d 22–42) by analysis or the basal diets supplemented with 110 mg Mn kg^–1 (d 1–21) and 80 mg Mn kg^–1(d 22–42) as either the Mn sulfate or the Mn proteinate with moderate chelation strength (Mn-Prot M) for 42 d. The results showed that the interaction between dietary Mn addition and gender had no impact (P>0.05) on any of the measured parameters; abdominal fat percentage of broilers was decreased (P<0.003) by Mn addition; Mn addition increased (P<0.004) adipose triglyceride lipase (ATGL) activity, while Mn-Prot M decreased (P<0.002) the fatty acid synthase (FAS) activity in the abdominal fat of broilers compared to the control; Mn addition decreased (P<0.009) diacylglycerol acyltransferase 2 (DGAT2) mRNA expression level and peroxisome proliferator-activated receptor γ (PPARγ) mRNA and protein expression levels, but up-regulated (P<0.05) the ATGL mRNA and protein expression levels in the abdominal fat of broilers. It was concluded that dietary supplementation with Mn inhibited the abdominal fat deposition of broilers possibly via decreasing the expression of PPARγ and DGAT2 as well as increasing the expression and activity of ATGL in the abdominal fat of broilers, and Mn-Prot M was more effective in inhibiting the FAS acitivity.

Key words: manganese , abdominal fat , broiler , gene expression , enzyme activity

Xiaoyan Cui, Ke Yang, Weiyun Zhang, Liyang Zhang, Ding Li, Wei Wu, Yun Hu, Tingting Li, Xugang Luo. 饲粮添加锰对肉鸡腹脂沉积的影响[J]. Journal of Integrative Agriculture, 2024, 23(12): 4161-4171.

Xiaoyan Cui, Ke Yang, Weiyun Zhang, Liyang Zhang, Ding Li, Wei Wu, Yun Hu, Tingting Li, Xugang Luo. Dietary manganese supplementation inhibits abdominal fat deposition possibly by regulating gene expression and enzyme activity involved in lipid metabolism in the abdominal fat of broilers[J]. Journal of Integrative Agriculture, 2024, 23(12): 4161-4171.

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饲粮添加锰对肉鸡腹脂沉积的影响

Dietary manganese supplementation inhibits abdominal fat deposition possibly by regulating gene expression and enzyme activity involved in lipid metabolism in the abdominal fat of broilers

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