非经典分泌蛋白（MoMtp）是稻瘟病菌产孢、应激稳态以及致病力所必需的

doi:10.1016/j.jia.2023.06.028

摘要/Abstract

摘要：

稻瘟病是由半活体营养子囊真菌稻瘟病菌（Magnaporthe oryzae）引起的病害，对全球水稻的可持续生产构成重大威胁。研究表明，为了成功地侵染，稻瘟病菌会向水稻细胞分泌大量功能多样的蛋白质以利其致病。然而，这些效应蛋白进入宿主细胞后靶向何处及其如何促进病原菌致病的机制仍不清。我们研究发现稻瘟病菌一假定非典型分泌蛋白MoMtp正向调控稻瘟病菌分生孢子产生和附着胞的形成；MoMTP基因敲除突变体在侵染水稻叶片时会引起水稻超敏反应，表明MoMtp对稻瘟病菌的毒性至关重要；细胞壁完整性和氧化胁迫实验结果表明MoMtp可能是真菌维持细胞结构完整性必须的；MoMTP基因在侵染的所有阶段均上调表达，表明其可能在稻瘟病菌对宿主入侵和定殖过程中起调节作用。此外，通过烟草瞬时表达以及水稻叶鞘侵染实验发现，MoMtp-GFP定位于烟草及水稻细胞的线粒体中。综上所述，我们推论MoMtp蛋白可以促进稻瘟病菌分正常的分生孢子的形成和致病，并可能在侵入过程中干扰水稻线粒体的正常功能。

Abstract:

Blast disease, caused by the hemibiotrophic ascomycete fungus, Magnaporthe oryzae, is a significant threat to sustainable rice production worldwide. Studies have shown that the blast fungus secretes vast arrays of functionally diverse proteins into the host cell for a successful disease progression. However, the final destinations of these effector proteins inside the host cell and their role in advancing fungal pathogenesis remain a mystery. Here, we reported that a putative mitochondrial targeting non-classically secreted protein (MoMtp) positively regulates conidiogenesis and appressorium maturation in M. oryzae. Moreover, MoMTP gene deletion mutant strains triggered a hypersensitive response when inoculated on rice leaves displaying that MoMtp is essential for the virulence of M. oryzae. In addition, cell wall and oxidative stress results indicated that MoMtp is likely involved in the maintenance of the structural integrity of the fungus cell. Our study also demonstrates an upregulation in the expression pattern of the MoMTP gene at all stages of infection, indicating its possible regulatory role in host invasion and the infectious development of M. oryzae. Furthermore, Agrobacterium infiltration and sheath inoculation confirmed that MoMtp-GFP protein is predominantly localized in the host mitochondria of tobacco leaf and rice cells. Taken together, we conclude that MoMtp protein likely promotes the normal conidiation and pathogenesis of M. oryzae and might have a role in disturbing the proper functioning of the host mitochondria during pathogen invasion.

Key words: plant-pathogen interactions , effectors , hypersensitive response , pathogenesis , mitochondria

Wajjiha Batool, Justice Norvienyeku, Wei Yi, Zonghua Wang, Shihong Zhang, Lili Lin. 非经典分泌蛋白（MoMtp）是稻瘟病菌产孢、应激稳态以及致病力所必需的[J]. Journal of Integrative Agriculture, 2024, 23(8): 2686-2702.

Wajjiha Batool, Justice Norvienyeku, Wei Yi, Zonghua Wang, Shihong Zhang, Lili Lin. Disruption of non-classically secreted protein (MoMtp) compromised conidiation, stress homeostasis, and pathogenesis of Magnaporthe oryzae[J]. Journal of Integrative Agriculture, 2024, 23(8): 2686-2702.

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