MYB转录因子的系统分析及LuMYB216在亚麻花花色苷生物合成调控中的作用

doi:10.1016/j.jia.2023.04.046

Journal of Integrative Agriculture ›› 2023, Vol. 22 ›› Issue (8): 2335-2345.DOI: 10.1016/j.jia.2023.04.046

MYB转录因子的系统分析及LuMYB216在亚麻花花色苷生物合成调控中的作用

收稿日期:2023-01-23 接受日期:2023-04-10 出版日期:2023-08-20 发布日期:2023-04-10

Systematic analysis of MYB transcription factors and the role of LuMYB216 in regulating anthocyanin biosynthesis in the flowers of flax (Linum usitatissimum L.)

XIE Dong-wei¹, LI Jing¹, ZHANG Xiao-yu², DAI Zhi-gang², ZHOU Wen-zhi³, SU Jian-guang², SUN Jian^1#

¹ School of Life Science, Nantong University, Nantong 226019, P.R.China

²Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410221, P.R.China
³ Jiangsu Sanshu Biotechnology Co. Ltd., Nantong 226001, P.R.China

Received:2023-01-23 Accepted:2023-04-10 Online:2023-08-20 Published:2023-04-10
About author:XIE Dong-wei, E-mail: xiedongwei@163.com; #Correspondence SUN Jian, Tel: +86-513-85012818, E-mail: sunjian8416@163.com
Supported by:
This work was supported by the National Natural Science Foundation of China (31801409), the Innovation and Entrepreneurship Training Program for College Students, Jiangsu Province, China (202210304103Y), and the Science and Technology Project of Nantong City, Jiangsu Province, China (JC2020156). We thank all the people who participated in this research.

摘要/Abstract

摘要：

花色苷是影响植物颜色和营养品质的重要色素。MYBs在植物花色苷的合成和积累中起着重要作用。然而，MYB转录因子在亚麻花的花色苷合成中的调节作用尚不清楚。在本研究中，从亚麻基因组中共鉴定出402个MYB转录因子，这些MYB成员在15条染色体上分布不均。其中，R2R3-LuMYB成员被分为32个亚家族。实时荧光定量（qRT-PCR）分析显示，进化树相邻亚家族中的7个R2R3-LuMYB基因具有相似的表达模式，其中LuMYB216在不同颜色的花瓣中高度表达。此外，对LuMYB216在亚麻中进行基因编辑后，观察到突变体植物的花瓣颜色、花药颜色和种皮颜色明显浅于野生型植株。测定lumyb216突变体植株的花瓣和种皮的花色苷，发现含量较野生型显著降低。相关分析表明，LuMYB216与其上游调控因子bHLH30共表达，且显著正相关。本研究系统分析了亚麻中的MYB基因家族，为研究LuMYB216在亚麻花的花色苷合成中的调控奠定了基础。

Abstract:

Anthocyanin is an important pigment that affects plant color and nutritional quality. MYBs play an important role in plant anthocyanin synthesis and accumulation. However, the regulatory function of MYB transcription factors in anthocyanin synthesis in flax flowers is still unclear. In this study, 402 MYB transcription factors were identified in the flax genome. These MYB members are unevenly distributed on 15 chromosomes. The R2R3-LuMYB members were divided into 32 phylogenetic subfamilies. qRT-PCR analysis showed that seven R2R3-LuMYB genes in the adjacent subfamily of the evolutionary tree had similar expression patterns, among which LuMYB216 was highly expressed in the petals of different colors. Moreover, gene editing of LuMYB216 in flax showed that the petal color, anther color and seed coat color of mutant plants were significantly lighter than those of wild-type plants, and the anthocyanin content of lumyb216 mutant plants was significantly reduced. Correlation analysis indicated that LuMYB216 was significantly positively correlated with the upstream regulator bHLH30. This study systematically analyzed the MYB gene family in flax, laying a foundation for studying the regulation of LuMYB216 in flax flower anthocyanin synthesis.

Key words: flax , MYB gene family , anthocyanin biosynthesis , LuMYB216 , functional characterization

XIE Dong-wei, LI Jing, ZHANG Xiao-yu, DAI Zhi-gang, ZHOU Wen-zhi, SU Jian-guang, SUN Jian.

MYB转录因子的系统分析及LuMYB216在亚麻花花色苷生物合成调控中的作用 [J]. Journal of Integrative Agriculture, 2023, 22(8): 2335-2345.

XIE Dong-wei, LI Jing, ZHANG Xiao-yu, DAI Zhi-gang, ZHOU Wen-zhi, SU Jian-guang, SUN Jian. Systematic analysis of MYB transcription factors and the role of LuMYB216 in regulating anthocyanin biosynthesis in the flowers of flax (Linum usitatissimum L.)[J]. Journal of Integrative Agriculture, 2023, 22(8): 2335-2345.

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MYB转录因子的系统分析及LuMYB216在亚麻花花色苷生物合成调控中的作用

Systematic analysis of MYB transcription factors and the role of LuMYB216 in regulating anthocyanin biosynthesis in the flowers of flax (Linum usitatissimum L.)

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