Journal of Integrative Agriculture ›› 2024, Vol. 23 ›› Issue (1): 77-92.DOI: 10.1016/j.jia.2023.04.023
普通小麦3B染色体粒重主效QTL的遗传解析与验证
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收稿日期:2023-01-03接受日期:2023-02-20出版日期:2024-01-20发布日期:2024-01-05
Genetic dissection and validation of a major QTL for grain weight on chromosome 3B in bread wheat (Triticum aestivum L.)
Simin Liao1, 2,
Zhibin Xu1, Xiaoli Fan1, Qiang Zhou1, Xiaofeng
Liu1, 2, Cheng Jiang1, 2, Liangen Chen1, 2,
Dian Lin1, 2, Bo Feng1#, Tao Wang1, 3
1 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
2 University of Chinese Academy of Sciences, Beijing 100049, China
3 The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, China
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Received:2023-01-03Accepted:2023-02-20Online:2024-01-20Published:2024-01-05 -
About author:Simin Liao, E-mail: Liaosm@cib.ac.cn;#Correspondence Bo Feng, Tel: +86-28-82890896, E-mail: fengbo@cib.ac.cn -
Supported by:This work was supported by the Major Program of National Agricultural Science and Technology of China (NK20220607), the West Light Foundation of the Chinese Academy of Sciences (2022XBZG_XBQNXZ_A_001), and the Sichuan Science and Technology Program, China (2022ZDZX0014).
摘要：
千粒重是小麦产量的重要组分之一。育种中，粒重位点的叠加可以有效提升小麦的产量潜力。本研究利用W7268×川育12重组自交系群体对千粒重、粒长、粒宽和长宽比进行了QTL分析，共检测到7个主效QTL (QGl.cib-2D, QGw.cib-2D, QGw.cib-3B, QGw.cib-4B.1, QGlw.cib-2D.1, QTgw.cib-2D.1 和 QTgw.cib-3B.1)，解释了2.61-34.85%的表型变异率。其中，两个主效粒重QTL和3个主效粒宽QTL分别存在显著的互作。QTgw.cib-3B.1和QGw.cib-3B共定位于3B染色体，且该位点的千粒重变异主要由粒宽贡献。与其它主效QTL不同，QTgw.cib-3B.1和 QGw.cib-3B对穗粒数没有显著影响。利用所开发的竞争性等位基因特异性PCR (KASP) 标记在高代系中对上述位点进行了验证。通过与前人的结果相比较，发现QTgw.cib-3B.1和 QGw.cib-3B是一个新的控制粒重的QTL位点。在候选区间内，共检测到6种单倍型且其在地方品种与栽培品种分布频率不同。此外，通过基因注释、表达谱分析、同源分析和序列分析，预测了QTgw.cib-3B.1/QGw.cib-3B的候选基因。本研究所报道的主效QTL和开发的KASP标记可为解析粒重的遗传基础和分子标记辅助育种提高小麦产量奠定了基础。
引用本文
. 普通小麦3B染色体粒重主效QTL的遗传解析与验证[J]. Journal of Integrative Agriculture, 2024, 23(1): 77-92.
Simin Liao, Zhibin Xu, Xiaoli Fan, Qiang Zhou, Xiaofeng Liu, Cheng Jiang, Liangen Chen, Dian Lin, Bo Feng, Tao Wang.
Genetic dissection and validation of a major QTL for grain weight on chromosome 3B in bread wheat (Triticum aestivum L.) [J]. Journal of Integrative Agriculture, 2024, 23(1): 77-92.
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