microRNAs与mRNAs联合揭示不同施氮量下玉米中激素的合成和信号转导机制

doi:10.1016/j.jia.2023.02.016

Journal of Integrative Agriculture ›› 2023, Vol. 22 ›› Issue (9): 2673-2686.DOI: 10.1016/j.jia.2023.02.016

microRNAs与mRNAs联合揭示不同施氮量下玉米中激素的合成和信号转导机制

收稿日期:2022-09-20 接受日期:2022-12-22 出版日期:2023-09-20 发布日期:2023-09-14

Integrating microRNAs and mRNAs reveals the hormones synthesis and signal transduction of maize under different N rates

YUE Kai^{1, 2}, LI Ling-ling^{1, 2#}, XIE Jun-hong^{1, 2}, Zechariah EFFAH³,Sumera ANWAR^{4, 5}, WANG Lin-lin^{1, 2}, MENG Hao-feng^{1, 2}, LI Lin-zhi⁶

¹College of Agronomy, Gansu Agricultural University, Lanzhou 730070, P.R.China
²State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, P.R.China
³Council for Scientific and Industrial Research, Plant Genetic Resources Research Institute, Bunso 00233, Ghana
⁴Department of Biosciences, Durham University, Durham DH1 3LE, UK
⁵Department of Botany, Government College, Women University, Faisalabad 38000, Pakistan
⁶ College of Resources and Environment Science, Gansu Agricultural University, Lanzhou 730070, P.R.China

Received:2022-09-20 Accepted:2022-12-22 Online:2023-09-20 Published:2023-09-14
About author:#Correspondence LI Ling-ling, Tel: +86-931-7631234, E-mail: lill@gsau.edu.cn
Supported by:
This research was supported by the Major Special Research Projects in Gansu Province, China (22ZD6NA009), the State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, China (GSCS-2022-Z02), the National Natural Science Foundation of China (32260549), and the National Key R&D Program of China (2022YFD1900300).

摘要/Abstract

摘要：

氮肥对玉米籽粒发育的影响还未得到充分研究。microRNAs与mRNAs联合分析有助于加深我们对氮素调控玉米籽粒发育的理解。在本研究中，我们分析了不同施氮量（0 kg ha^-1、100 kg ha^-1，200 kg ha^-1和300 kg ha^-1）下玉米籽粒的形态、生理和转录组变化。结果表明，增加施氮显著增加了玉米籽粒的鲜重和干重，但施氮量超过200 kg ha^-1时，籽粒的鲜重和干重没有显著增加。总体来说，生长素、细胞分裂素和赤霉素的含量随着施氮量的增加而增加，而乙烯的含量降低。我们在激素合成和传导过程中获得了31个差异表达基因（DEGs），其中9个DEGs由14个差异表达microRNAs（DEMIs）调节，共形成26个表达对。本研究中候选的DEGs和DEMIs为不同施氮量下的玉米籽粒发育提供了有价值的见解。

Abstract: The effect of nitrogen (N) fertilizer on the development of maize kernels has yet to be fully explored. MicroRNA-mRNA analyses could help advance our understanding of how kernels respond to N. This study analyzed the morphological, physiological, and transcriptomic changes in maize kernels under different N rates (0, 100, 200, and 300 kg ha^–1). The result showed that increasing N application significantly increased maize grains’ fresh and dry weight until N reached 200 kg ha^–1. Higher levels of indole-3-acetic acid, cytokinin, gibberellin, and a lower level of ethylene were associated with increased N applications. We obtained 31 differentially expressed genes (DEGs) in hormone synthesis and transduction, and 9 DEGs were regulated by 14 differentially expressed microRNAs (DEMIs) in 26 pairs. The candidate DEGs and DEMIs provide valuable insight for manipulating grain filling under different N rates.

Key words: maize kernels , phytohormones , high-throughput sequencing , microRNA

YUE Kai, LI Ling-ling, XIE Jun-hong, Zechariah EFFAH, Sumera ANWAR, WANG Lin-lin, MENG Hao-feng, LI Lin-zhi. microRNAs与mRNAs联合揭示不同施氮量下玉米中激素的合成和信号转导机制[J]. Journal of Integrative Agriculture, 2023, 22(9): 2673-2686.

YUE Kai, LI Ling-ling, XIE Jun-hong, Zechariah EFFAH, Sumera ANWAR, WANG Lin-lin, MENG Hao-feng, LI Lin-zhi. Integrating microRNAs and mRNAs reveals the hormones synthesis and signal transduction of maize under different N rates[J]. Journal of Integrative Agriculture, 2023, 22(9): 2673-2686.

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microRNAs与mRNAs联合揭示不同施氮量下玉米中激素的合成和信号转导机制

Integrating microRNAs and mRNAs reveals the hormones synthesis and signal transduction of maize under different N rates

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