JIA-2021-0802 二倍体林地草莓脂氧合酶（LOX）基因家族全基因组鉴定及其在非生物胁迫下表达分析

doi:10.1016/S2095-3119(21)63819-4

摘要/Abstract

摘要：

本研究从二倍体森林草莓基因组中鉴定出14个LOX基因。系统发育树将FvLOX基因分为9-LOX和13-LOX两个亚家族。基因重复事件分析表明，全基因组复制/片段复制和分散复制有效地促进了草莓LOX家族的扩展。QRT-PCR分析表明，FvLOX基因在不同组织中均有不同程度的表达。表达谱分析表明，FvLOX1和FvLOX8在低温胁迫下表达上调，FvLOX3和FvLOX7在干旱胁迫下表达上调，FvLOX6和FvLOX9在盐胁迫下表达上调，FvLOX2、FvLOX3和FvLOX6在SA处理下表达上调，FvLOX3、FvLOX11和FvLOX14在MeJA处理下表达上调，FvLOX4和FvLOX14在ABA处理下表达上调。启动子分析表明，FvLOX基因参与植物生长发育和胁迫响应。我们对草莓FvLOX家族进行了全基因组分析和鉴定，表征了多种参与非生物胁迫响应的FvLOX候选基因，该研究为草莓在非生物胁迫中的响应机制奠定了理论和经验基础。

Abstract: Lipoxygenase (LOXs) is a kind of dioxygenase without heme and iron, which plays an important role in the development and adaptation of many plants to the environment. However, the study of strawberry LOX gene family has not been reported. In this study, 14 LOX genes were identified from the diploid woodland strawberry genome. The phylogenetic tree divides the FvLOX gene into two subfamilies: 9-LOX and 13-LOX. Gene duplication event analysis showed that whole-genome duplication (WGD)/segmental duplication and dispersed duplication effectively promoted the expansion of strawberry LOX family. QRT-PCR analysis showed that FvLOX genes were expressed in different tissues. Expression profile analysis showed that FvLOX1 and FvLOX8 were up-regulated under low temperature stress, FvLOX3 and FvLOX7 were up-regulated under drought stress, FvLOX6 and FvLOX9 were up-regulated under salt stress, FvLOX2, FvLOX3 and FvLOX6 were up-regulated under salicylic acid (SA) treatment, FvLOX3, FvLOX11 and FvLOX14 were up-regulated under methyl jasmonate (MeJA) treatment, FvLOX4 and FvLOX14 were up-regulated under abscisic acid (ABA) treatment. Promoter analysis showed that FvLOX genes were involved in plant growth and development and stress response. We analyzed and identified the whole genome of strawberry FvLOX family and characterized a variety of FvLOX candidate genes involved in abiotic stress response. This study laid a theoretical and empirical foundation for the response mechanism of strawberry to abiotic stress.

Key words: lipoxygenase , gene family , strawberry , abiotic stress , expression pattern , cis-acting element

. JIA-2021-0802 二倍体林地草莓脂氧合酶（LOX）基因家族全基因组鉴定及其在非生物胁迫下表达分析[J]. Journal of Integrative Agriculture, 2022, 21(7): 1982-1996.

LI Zhi-qi, Xie Qian, YAN Jia-hui, CHEN Jian-qing, CHEN Qing-xi. Genome-wide identification and characterization of the abiotic-stress-responsive lipoxygenase gene family in diploid woodland strawberry (Fragaria vesca)[J]. Journal of Integrative Agriculture, 2022, 21(7): 1982-1996.

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