黄/褐籽甘蓝型油菜种子的转录组分析比较类黄酮和脂肪酸含量变化的分子调控

doi:10.1016/S2095-3119(21)63656-0

Journal of Integrative Agriculture ›› 2022, Vol. 21 ›› Issue (8): 2211-2226.DOI: 10.1016/S2095-3119(21)63656-0

所属专题：油料作物合辑Oil Crops

黄/褐籽甘蓝型油菜种子的转录组分析比较类黄酮和脂肪酸含量变化的分子调控

收稿日期:2020-11-13 接受日期:2021-02-22 出版日期:2022-08-01 发布日期:2021-02-22

Transcriptional profiling between yellow- and black-seeded Brassica napus reveals molecular modulations on flavonoid and fatty acid content

RONG Hao¹, YANG Wen-jing¹, XIE Tao¹, WANG Yue¹, WANG Xia-qin¹, JIANG Jin-jin¹, WANG You-ping^{1, 2}

¹ Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou 225009, P.R.China
²Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education of China, Yangzhou University, Yangzhou 225009, P.R.China

Received:2020-11-13 Accepted:2021-02-22 Online:2022-08-01 Published:2021-02-22
About author:Correspondence WANG You-ping, Tel: +86-514-87997303, E-mail: wangyp@yzu.edu.cn; JIANG Jin-jin, E-mail: jjjiang@yzu.edu.cn
Supported by:
This research was funded by the National Natural Science Foundation of China (U20A2028 and 31972963), the Open Funds of the Key Laboratory of Plant Functional Genomics of the Ministry of Education, China (ML201804), the Project of Special Funding for Crop Science Discipline Development, China (yzuxk202006), the Priority Academic Program Development of Jiangsu Higher Education Institutions, China and the Yangzhou University for Excellent Talent Support Program, China.

摘要/Abstract

摘要：

本研究通过RNA-seq分析比较了甘蓝型油菜-白芥属间杂种后代的黄籽材料及其褐籽亲本间的基因表达差异，并与类黄酮和脂肪酸含量变化进行关联分析。通过HPLC-PDA-ESI(−)/MSⁿ分析，我们发现黄籽中苯丙烷和类黄酮类物质（如异鼠李素、表儿茶素、山奈酚和其它衍生物）的含量显著低于褐籽材料。黄籽材料的脂肪酸含量较褐籽高，主要是由于C16:0、C18:0、C18:1、C18:2和C18:3的含量变化所导致。通过授粉后4周（4 WAF）和5周种子的RNA-seq分析，我们发现黄、褐籽间的差异表达基因（DEGs）主要富集于类黄酮和脂肪酸合成相关的路径，包括BnTT3、BnTT4、BnTT18和BnFAD2。此外，我们发现黄籽中脂肪酸合成、去饱和、延伸相关的基因（FAD3、LEC1、FUS3、LPAT2）较褐籽上调表达，而与β氧化相关的基因（AIM1和KAT2）在黄籽中下调表达。这些与类黄酮、苯丙烷、脂肪酸含量变化相关的DEGs将有助于解释黄籽甘蓝型油菜的表型变化，且对于油菜的遗传改良也具有一定的意义。

Abstract:

Brassica napus is an important cash crop broadly grown for the vegetable and oil values. Yellow-seeded B. napus is preferred by breeders due to its improved oil and protein quality, less pigments and lignin compared with the black-seeded counterpart. This study compared the differences in flavonoid and fatty acid contents between yellow rapeseed from the progenies of B. napus–Sinapis alba somatic hybrids and the black-seeded counterpart using RNA-seq analysis. Through HPLC-PDA-ESI(−)/MS² analysis, it was found that phenylpropanoids and flavonoids (i.e., isorhamnetin, epicatechin, kaempferol, and other derivatives) in yellow seed were significantly lower than those in black seed. The fatty acid (FA) content in yellow rapeseed was higher than that in black rapeseed due to the variation of C16:0, C18:0, C18:1, C18:2, and C18:3 contents. RNA-seq analysis of seeds at four and five weeks after flowering (WAF) indicated that differentially expressed genes (DEGs) between black and yellow rapeseeds were enriched in flavonoid and FA biosynthesis, including BnTT3, BnTT4, BnTT18, and BnFAD2. Also, genes related to FA biosynthesis, desaturation and elongation (FAD3, LEC1, FUS3, and LPAT2) in yellow seed were up-regulated compared to those in black seed, while genes involved in beta-oxidation cycle (AIM1 and KAT2) of yellow seed were down-regulated compared to those in black seed. The DEGs related to the variation of flavonoids, phenylpropanoids, and FAs would help improve the knowledge of yellow seed character in B. napus and promote rapeseed improvement.

Key words: rapeseed , gene expression analysis , fatty acid composition , phenolic content , yellow seed

. 黄/褐籽甘蓝型油菜种子的转录组分析比较类黄酮和脂肪酸含量变化的分子调控[J]. Journal of Integrative Agriculture, 2022, 21(8): 2211-2226.

RONG Hao, YANG Wen-jing, XIE Tao, WANG Yue, WANG Xia-qin, JIANG Jin-jin, WANG You-ping. Transcriptional profiling between yellow- and black-seeded Brassica napus reveals molecular modulations on flavonoid and fatty acid content[J]. Journal of Integrative Agriculture, 2022, 21(8): 2211-2226.

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黄/褐籽甘蓝型油菜种子的转录组分析比较类黄酮和脂肪酸含量变化的分子调控

Transcriptional profiling between yellow- and black-seeded Brassica napus reveals molecular modulations on flavonoid and fatty acid content

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