小麦DnaJ家族的全基因组鉴定及其对小麦黄花叶病毒侵染的调控分析

doi:10.1016/S2095-3119(21)63619-5

Journal of Integrative Agriculture ›› 2022, Vol. 21 ›› Issue (1): 153-169.DOI: 10.1016/S2095-3119(21)63619-5

小麦DnaJ家族的全基因组鉴定及其对小麦黄花叶病毒侵染的调控分析

收稿日期:2020-01-01 接受日期:2020-12-30 出版日期:2022-01-01 发布日期:2022-01-01

Genome-wide identification and analysis of the regulation wheat DnaJ family genes following wheat yellow mosaic virus infection

LIU Ting-ting¹, XU Miao-ze¹, GAO Shi-qi¹, ZHANG Yang², HU Yang³, JIN Peng¹, CAI Lin-na¹, CHENG Ye¹, CHEN Jian-ping1, YANG Jian¹, ZHONG Kai-li¹

¹ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products/Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Rural Affairs and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, P.R.China
² Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, P.R.China
³ Institute of Forest Health, Zhejiang Academy of Forestry, Hangzhou 310021, P.R.China

Received:2020-01-01 Accepted:2020-12-30 Online:2022-01-01 Published:2022-01-01
About author:LIU Ting-ting, E-mail: tl612427@foxmail.com; Correspondence YANG Jian, Tel: +86-574-87605539, E-mail: nather2008@163.com; ZHONG Kai-li, Tel: +86-574-87605521, E-mail: zhongkaili@nbu.edu.cn
Supported by:
This work was supported by the National Key R&D Program of China (2018YFD0200507, 2017YFD-0201701, and 2018YFD0200408), the National Natural Science Foundation of China (31901954), the Natural Science Foundation of Ningbo City, China (2019A610415 and 2019A610410), the National Key Project for Research on Transgenic Biology, China (2016ZX08002-001), the China Modern Agricultural Industry Technology System of MOF and MARA (CARS-03), and the K.C. Wong Magna Funding in Ningbo University, China.

摘要/Abstract

摘要：

DnaJ蛋白最初是在大肠杆菌中鉴定的一种大小约为41kDa的热休克蛋白，其蛋白家族是分子伴侣中最多样化的家族，在蛋白质折叠和各种生理活动的调节中扮演了重要角色，且在植物发育和胁迫应答中发挥重要作用。DnaJ家族蛋白已在许多物种中广泛研究，例如人类，果蝇，蘑菇，西红柿和拟南芥等，但其在小麦中的作用以及其与植物病毒之间的相互作用机制却鲜少有研究。在这篇文章中，我们鉴定了236个TaDnaJs，并对其保守结构域，基因结构、蛋白质基序、蛋白质结构、染色体定位和共线性以及顺式作用元件进行了全基因组分析。根据分析结果，我们将这些TaDnaJs按其结构域分成DJA、DJB和DJC三组，并从分组中随机选择了6个基因进行组织特异性分析和激素胁迫下的基因表达谱分析，结果表明TaDnaJ基因在不同组中存在组织差异表达，DJA组的基因表达集中在顶部叶片，对ABA和GA更为敏感；DJB组的基因表达水平在根和种子中最高，对ABA更为敏感；DJC组中的基因表达在小麦叶片中最高，其次是根和种子，对SA和GA最敏感。另外我们随机选择了17个基因分析植物病毒侵染后基因表达水平的变化，结果显示，在测试的17个TaDnaJ基因中，有16个基因在小麦黄花叶病毒（WYMV）侵染后呈现上调表达，这表明TaDnaJ家族可能参与了植物防御反应。随后我们通过酵母两杂交实验验证了WYMV NIa、NIb和7KD蛋白与WYMV侵染后表达水平变化最显著的TraesCS7A02G506000相互作用。在这篇文章中，我们探究了DnaJ蛋白介导的胁迫耐受性和敏感性的分子机制，DnaJ基因可能参与了植物对非生物和生物胁迫的抗性。本研究提高了对TaDnaJ基因表达谱的认识，并且为TaDnaJ家族与植物防御机制之间的研究提供了一定的研究基础。

Abstract: The co-chaperone DnaJ plays an important role in protein folding and regulation of various physiological activities, and participates in several pathological processes. DnaJ has been extensively studied in many species including humans, drosophila, mushrooms, tomatoes, and Arabidopsis. However, few studies have examined the role of DnaJ in wheat (Triticum aestivum), and the interaction mechanism between TaDnaJs and plant viruses. Here, we identified 236 TaDnaJs and performed a comprehensive genome-wide analysis of conserved domains, gene structure and protein motifs, chromosomal positions and duplication relationships, and cis-acting elements. We grouped these TaDnaJs according to their domains, and randomly selected six genes from the groups for tissue-specific analysis, and expression profiles analysis under hormone stress, and 17 genes for plant virus infection stress. In qRT-PCR, we found that among the 17 TaDnaJ genes tested, 16 genes were up-regulated after wheat yellow mosaic virus (WYMV) infection, indicating that the TaDnaJ family is involved in plant defense response. Subsequent yeast two-hybrid assays verified the WYMV NIa, NIb and 7KD proteins interacted with TaDJC (TraesCS7A02G506000), which had the most significant changes in gene expression levels after WYMV infection. Insights into the molecular mechanisms of TaDnaJ-mediated stress tolerance and sensitivity could inform different strategies designed to improve crop resistance to abiotic and biotic stress. This study provides a basis for future investigation of the TaDnaJ family and plant defense mechanisms.

Key words: TaDnaJ , WYMV , wheat , genome-wide , expression , hormone , biotic stress , HSP40

. 小麦DnaJ家族的全基因组鉴定及其对小麦黄花叶病毒侵染的调控分析[J]. Journal of Integrative Agriculture, 2022, 21(1): 153-169.

LIU Ting-ting, XU Miao-ze, GAO Shi-qi, ZHANG Yang, HU Yang, JIN Peng, CAI Lin-na, CHENG Ye, CHEN Jian-ping, YANG Jian, ZHONG Kai-li. Genome-wide identification and analysis of the regulation wheat DnaJ family genes following wheat yellow mosaic virus infection[J]. Journal of Integrative Agriculture, 2022, 21(1): 153-169.

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小麦DnaJ家族的全基因组鉴定及其对小麦黄花叶病毒侵染的调控分析

Genome-wide identification and analysis of the regulation wheat DnaJ family genes following wheat yellow mosaic virus infection

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