利用AFLP和SSR标记构建枸杞遗传连锁图谱

doi:10.1016/S2095-3119(21)63610-9

Journal of Integrative Agriculture ›› 2022, Vol. 21 ›› Issue (1): 131-138.DOI: 10.1016/S2095-3119(21)63610-9

所属专题：园艺-分子生物合辑Horticulture — Genetics · Breeding

利用AFLP和SSR标记构建枸杞遗传连锁图谱

收稿日期:2020-05-28 接受日期:2020-12-23 出版日期:2022-01-01 发布日期:2022-01-01

Constructing the wolfberry (Lycium spp.) genetic linkage map using AFLP and SSR markers

YIN Yue^{1, 2}, AN Wei², ZHAO Jian-hua², LI Yan-long², FAN Yun-fang², CHEN Jin-huan³, CAO You-long², ZHAN Xiang-qiang¹

¹ State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling 712100, P.R.China
² National Wolfberry Engineering Research Center, Ningxia Academy of Agricultural and Forestry Sciences, Yinchuan 751002,
P.R.China
³ College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, P.R.China

Received:2020-05-28 Accepted:2020-12-23 Online:2022-01-01 Published:2022-01-01
About author:YIN Yue, E-mail: yueyin0112@aliyun.com; Correspondence CAO You-long, Tel/Fax: +86-951-6886785, E-mail: youlongchk@163.com; ZHAN Xiang-qiang, Tel/Fax: +86-29-87082613, E-mail: zhanxq77@nwsuaf.edu.cn
Supported by:
This research was financially supported by the National Natural Science Foundation of China (31760218), the Third Batch of Ningxia Youth Talents Supporting Program (TJGC2018022) and the Whole Industry Chain Innovation Demonstration Project of Ningxia Academy of Agriculture and Forestry Sciences, China (ERS-2016-0405).

摘要/Abstract

摘要：

遗传连锁图谱在数量性状位点和分子标记辅助选择育种中具有重要意义。枸杞是我国重要的药食同源植物。然而，由于缺乏基因组和遗传资源，枸杞遗传连锁图谱的构建报到很少。在本研究中，采用双假测交理论，以‘北方枸杞’为母本，‘宁夏黄果’为父本杂交获得89株F1群体为试材，利用SSR和AFLP技术构建枸杞的分子遗传连锁图谱。共获得12个连锁群，包含165个标记位点（74个AFLP和91个SSR），覆盖基因组557.6cM，标记间平均图距为3.38cM。每个连锁群的标记数在3~12个，每个连锁群长度为8.6~58.3cM。连锁群上有29个偏分离标记，主要集中LG4和LG9上。这是第一张利用SSR和AFLP标记构建的枸杞属植物遗传连锁图谱，可为枸杞属植物遗传育种改良和辅助基因组组装提供理论依据。

Abstract: Genetic linkage maps are important for quantitative trait locus (QTL) and marker-assisted selection breeding. The wolfberry (Lycium spp.) is an important food and traditional medicine in China. However, few construction genetic linkage maps have been reported because of the lack of genomic and genetic resources. In this study, a population of 89 F₁ seedings was derived from a cross between two heterozygous parents, L. chinense var. potaninii ‘BF-01’ (female) and L. barbarum var. auranticarpum ‘NH-01’ (male), in order to construct a genetic linkage map using simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) markers based on the double pseudo-test cross mapping strategy. The resulting genetic map consisted of 165 markers (74 AFLPs and 91 SSRs) distributed across 12 linkage groups and spanned a total length of 557.6 cM with an average distance of 3.38 cM between adjacent markers. The 12 linkage groups contained 3 to 21 markers and ranged in length from 8.6 to 58.3 cM. Twenty-nine segregated markers distributed in the map were mainly located on LG4 and LG9 linkage groups at P<0.05. This is the first linkage map of Lycium species using SSR and AFLP markers, which can serve as basis for improving genes and selective breeding of the genome assembly.

Key words: Lycium , genetic linkage map , AFLP , SSR

. 利用AFLP和SSR标记构建枸杞遗传连锁图谱[J]. Journal of Integrative Agriculture, 2022, 21(1): 131-138.

YIN Yue, AN Wei, ZHAO Jian-hua, LI Yan-long, FAN Yun-fang, CHEN Jin-huan, CAO You-long, ZHAN Xiang-qiang. Constructing the wolfberry (Lycium spp.) genetic linkage map using AFLP and SSR markers[J]. Journal of Integrative Agriculture, 2022, 21(1): 131-138.

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利用AFLP和SSR标记构建枸杞遗传连锁图谱

Constructing the wolfberry (Lycium spp.) genetic linkage map using AFLP and SSR markers

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