水稻半显性反温敏核不育基因RTMS10的鉴定与精细定位

doi:10.1016/S2095-3119(20)63563-8

Journal of Integrative Agriculture ›› 2022, Vol. 21 ›› Issue (2): 316-325.DOI: 10.1016/S2095-3119(20)63563-8

所属专题：水稻遗传育种合辑Rice Genetics · Breeding · Germplasm Resources

水稻半显性反温敏核不育基因RTMS10的鉴定与精细定位

收稿日期:2020-05-23 接受日期:2020-11-24 出版日期:2022-01-02 发布日期:2022-01-02

Characterization and fine mapping of RTMS10, a semi-dominant reverse thermo-sensitive genic male sterile locus in rice

NI Jin-long^{1, 2}, WANG De-zheng², NI Da-hu², SONG Feng-shun², YANG Jian-bo², YAO Da-nian¹

¹ College of Agronomy, Anhui Agricultural University, Hefei 230036, P.R.China
² Rice Genetics and Breeding Key Laboratory of Anhui Province/Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, P.R.China

Received:2020-05-23 Accepted:2020-11-24 Online:2022-01-02 Published:2022-01-02
About author:Received 23 May, 2020 Accepted 24 November, 2020 Correspondence NI Jin-long, Tel: +86-551-65160535, E-mail: jlni09@163.com; YANG Jian-bo, Tel: +86-551-65160535, E-mail: yjianbo@263.net; YAO Da-nian, E-mail: dnyao@163.com
Supported by:
This research was funded by the Key Research and Development Program of Anhui Province, China (201904a06020016 and 202104g01020013), the National Natural Science Foundation of China (31101204) and the Program of Rice Genetic Breeding Key Laboratory of Anhui Province, China (SDKF-201903).

摘要/Abstract

摘要：

环境敏感型水稻雄性不育资源的发现与利用，大大便捷了杂交水稻育种，且为杂交水稻生产做出了重要贡献。在两系杂交水稻系统中，光敏和温敏核不育系大多表现为长日高温雄性不育，短日低温育性恢复。然而，反温敏核不育系YnS表现为低温（<29℃）雄性不育，高温（>29.5℃）可育。本研究报道了YnS反温敏核不育性状（rTGMS）的遗传规律及基因定位。YnS与正常可育材料L422杂交F1在低温22℃表现为雄性不育，在27℃条件下育性完全恢复。在YnS/L422 F2群体中，可育单株与不育单株的分离比随环境逐渐升高由1:3.05转变为2.95:1，表明rTGMS呈半显性遗传。通过BSA法分析，在第10号染色体上定位到1个与rTGMS紧密关联的主效位点，命名为RTMS10。利用YnS/L422 BC6F2（L422为轮回亲本）群体，将RTMS10精细定位在标记ID13116和ID1318之间~68kb的物理区间内。结合分子标记辅助选择，在YnS/L422 BC6F3世代获得1个L422背景的携带RTMS10的近等基因系，NL1。组织细胞学切片和扫描电镜分析发现，在低温（22℃）条件下，NL1花粉发育在减数分裂期发生明显异常。上述结果有助于对rTGMS性状和机制的理解。本研究还报道了该基因的共分离标记，将为加速反温敏核不育系的分子选育提供技术依据。

Abstract: The discovery and application of environment-sensitive genic male sterile (EGMS) rice germplasm provide an easy method for hybrid rice breeding and have made great contributions to hybrid rice production. Typically, the photoperiod- and thermo-sensitive GMS (P/TGMS) lines utilized in two-line hybrid systems are male sterile under long day or/and high temperature but fertile under short day or/and low temperature conditions. However, YannongS (YnS), a reverse TGMS (rTGMS) line, is sterile under low temperature (<29°C) and fertile under high temperature (>29.5°C). Here, we report a genetic study on the rTGMS trait in YnS. Interestingly, the F₁ plants of the cross between YnS and a cultivar, L422, were male sterile at 22°C and completely fertile at 27°C. Moreover, the segregation ratio of fertile and sterile individuals in YnS/L422 F₂ populations changed from 1:3.05 to 2.95:1 when the ambient temperature increased, showing that the rTGMS trait exhibits semi-dominance in YnS. We further found a locus on chromosome 10, termed RTMS10, which controls the rTGMS trait in YnS. We then finely mapped RTMS10 to a ~68 kb interval between markers ID13116 and ID1318 by YnS/L422 BC₆F₂ populations. A near iso-genic line (NIL) NL1 from the BC₆F₃ generation was developed and the pollen of NL1 became abnormal from the meiosis stage under low temperature. In summary, we identified an rTGMS locus, RTMS10, and provided co-segregated markers, which could help to accelerate molecular breeding of rTGMS lines and better understand the rTGMS trait in rice.

Key words: rice , photoperiod- and thermo-sensitive genic male sterile (P/TGMS) , reverse TGMS (rTGMS) , gene mapping , RTMS10

. 水稻半显性反温敏核不育基因RTMS10的鉴定与精细定位 [J]. Journal of Integrative Agriculture, 2022, 21(2): 316-325.

NI Jin-long, WANG De-zheng, NI Da-hu, SONG Feng-shun, YANG Jian-bo, YAO Da-nian. Characterization and fine mapping of RTMS10, a semi-dominant reverse thermo-sensitive genic male sterile locus in rice[J]. Journal of Integrative Agriculture, 2022, 21(2): 316-325.

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水稻半显性反温敏核不育基因RTMS10的鉴定与精细定位

Characterization and fine mapping of RTMS10, a semi-dominant reverse thermo-sensitive genic male sterile locus in rice

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