An improved scheme for infectious bursal disease virus genotype classification based on both genome-segments A and B

doi:10.1016/S2095-3119(20)63424-4

Journal of Integrative Agriculture ›› 2021, Vol. 20 ›› Issue (5): 1372-1381.DOI: 10.1016/S2095-3119(20)63424-4

收稿日期:2020-05-27 出版日期:2021-05-01 发布日期:2021-04-12

An improved scheme for infectious bursal disease virus genotype classification based on both genome-segments A and B

WANG Yu-long^{1, 2}, FAN Lin-jin^{1, 2}, JIANG Nan^{1, 2}, GAO Li^{1, 2}, LI Kai^{1, 2}, GAO Yu-long^{1, 2}, LIU Chang-jun¹, CUI Hong-yu¹, PAN Qing¹, ZHANG Yan-ping¹, WANG Xiao-mei^{1, 2, 3}, QI Xiao-le^{1, 2}

¹ Avian Immunosuppressive Diseases Division, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R.China
² OIE Reference Laboratory for Infectious Bursal Disease, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R.China
³ Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou 225009, P.R.China

Received:2020-05-27 Online:2021-05-01 Published:2021-04-12
Contact: Correspondence QI Xiao-le, Tel: +86-451-51051690, Fax: +86-451-51997166, E-mail: qixiaole@caas.cn; WANG Xiao-mei, E-mail: wangxiaomei@caas.cn
About author:WANG Yu-long, E-mail: xyylong@126.com;
Supported by:
This work was supported by the Natural Science Foundation of Heilongjiang Province, China (ZD2020C006 and TD2019C003), the National Key Research and Development Program of China (2016YFE0203200), the Heilongjiang Province Foundation for the National Key Research and Development Program of China (GX18B011), the Major Project of National Natural Science Foundation of China (31430087), and the earmarked fund for China Agriculture Research System (CARS-41-G15).

摘要/Abstract

摘要：

传染性法氏囊病（Infectious bursal disease, IBD）是一种严重危害养禽业健康发展的传染病，该病的病原是传染性法氏囊病病毒（Infectious bursal disease virus, IBDV）。IBDV的基因组由双节段双股RNA组成，即A节段和B节段。传统上，依据致病性和抗原性，IBDV可分为经典株、变异株、超强毒株和弱毒株。近年来，随着IBDV的不断变异，具有新的基因特征的IBDV毒株不断出现。传统的IBDV分类方法已不能涵盖这些新出现的毒株。因此，亟需建立一种新的IBDV基因型分类方法用于IBDV的流行病学研究。近年来，A节段基因序列常被用于IBDV的基因分类。然而，对于基因组分节段的IBDV来讲，A节段和B节段在病毒的遗传演化中都很重要，仅基于A节段基因序列的基因分类方法是不全面的。而且，原有的分类方法已经不能涵盖不断出现的IBDV节段重配病毒和最新出现的IBDV新型变异株。因此，本研究率先建立了一种兼顾IBDV基因组双节段特征的新的IBDV基因分型方法。在该分型系统中，基于A节段编码的VP2高变区核苷酸序列特征，IBDV被分为9个基因群（A1、A2、A3、A4、A5、A6、A7、A8和AII）；基于B节段编码的B-maker的核苷酸序列特征，IBDV被分为5个基因群（B1、B2、B3、B4和BII）；A2又被进一步分为4个亚群（A2a、A2b、A2c和A2d）。利用新的基因分型方法，传统的经典株、变异株、超强毒株和弱毒株分别被归类于基因群A1B1、A2B1、A3B2和A8B1。本研究中鉴定的IBDV新型变异株被归类为基因群A2dB1。本研究建立的IBDV基因分型方法，是一个灵活多样的开元系统，可用于现有毒株和新出现毒株的明确鉴定，将极大地方便IBDV的分子流行病学研究。

Abstract:

Infectious bursal disease (IBD) is caused by infectious bursal disease virus (IBDV), which has a genome consisting of two segments of double-stranded linear RNA. IBDVs have been traditionally divided into four phenotypes based on their pathogenicity and antigenicity, including classic, variant, very virulent, and attenuated IBDV. With the emergences of divergent molecular characteristics of novel strains produced by continuous mutations and recombination, it is increasingly difficult to define new IBDV strains using the traditional descriptive classification method. The most common classification scheme for IBDV with segmented genome is based solely on segment A, while the significance of segment B has been largely neglected. In this study, an improved scheme for IBDV genotype classification based on the molecular characteristics of both VP2 (a viral capsid protein encoded by segment A) and VP1 (an RNA-dependent RNA polymerase protein encoded by segment B) was proposed for the first time. In this scheme, IBDV was classified into nine genogroups of A and five genogroups of B, respectively; the genogroup A2 was further divided into four lineages. The commonly used phenotypic classifications of classic, variant, very virulent, and attenuated IBDVs correspond to the A1B1, A2B1, A3B2, and A8B1 genotypes of the proposed classification scheme. The novel variant IBDVs including the strains identified in this study were classified as belonging to genotype A2dB1. The flexibility and versatility of this improved classification scheme will allow the unambiguous identification of existing and emerging IBDV strains, which will greatly facilitate molecular epidemiology studies of IBDV.

Key words: infectious bursal disease virus , genotype , VP1 , VP2 , novel variant strain

. [J]. Journal of Integrative Agriculture, 2021, 20(5): 1372-1381.

WANG Yu-long, FAN Lin-jin, JIANG Nan, GAO Li, LI Kai, GAO Yu-long, LIU Chang-jun, CUI Hong-yu, PAN Qing, ZHANG Yan-ping, WANG Xiao-mei, QI Xiao-le. An improved scheme for infectious bursal disease virus genotype classification based on both genome-segments A and B[J]. Journal of Integrative Agriculture, 2021, 20(5): 1372-1381.

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An improved scheme for infectious bursal disease virus genotype classification based on both genome-segments A and B

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