小麦-华山新麦草渗入系H148中全蚀病抗性遗传位点的鉴定

doi:10.1016/S2095-3119(20)63340-8

摘要/Abstract

摘要：

小麦全蚀病 (Take-all) 是一种具有毁灭性的土传病害，培育抗病材料是控制该病害的重要途径之一。华山新麦草 (Psathyrostachys huashanica Keng) 是小麦品种改良的重要遗传资源，特别是小麦全蚀病稀缺的抗性资源。在本研究中，相比感病亲本7182，小麦-华山新麦草渗入系H148的全蚀病抗性得到了显著性提升。为了明确H148抗病性的遗传机制，我们构建了H148和西农585的F₂遗传分离群体，且利用植物数量遗传体系“主基因+多基因”混合遗传模型分离分析法对其研究发现，H148的全蚀病抗性受到两对主效基因的共同控制，这两对主效基因存在一定的加性、显性和上位性效应。同时，结合集群分离分析法 (Bulked Segregant Analysis, BSA) 和小麦660K基因芯片筛选出与抗病相关的外源特异性SNP，主要分布于小麦2A染色体。根据特异性SNP开发竞争性等位基因特异性PCR (Kompetitive allele specific PCR, KASP) 分子标记，对F₂群体进行基因分型，最终在2A染色体的68.8-70.1Mb区间内定位到一个主效的QTL。该目标区间在小麦参考基因组序列上存在62个候选基因，经基因功能注释显示，两个可编码蛋白的基因与系统性提升植物根系抗性相关，被预测可能参与了小麦对全蚀病的抗病反应。总之，小麦-华山新麦草渗入系H148的选育以及抗病QTL的定位，以期为小麦抗全蚀病分子辅助育种和抗病基因的精细定位提供一定的参考信息。

Abstract:

Take-all is a devastating soil-borne disease of wheat (Triticum aestivum L.). Cultivating resistant line is an important measure to control this disease. Psathyrostachys huashanica Keng is a valuable germplasm resource with high resistance to take-all. This study reported on a wheat-P. huashanica introgression line H148 with improved take-all resistance compared with its susceptible parent 7182. To elucidate the genetic mechanism of resistance in H148, the F₂ genetic segregating population of H148×XN585 was constructed. The mixed genetic model analysis showed that the take-all resistance was controlled by two major genes with additive, dominant and epistasis effects. Bulked segregant analysis combined with wheat axiom 660K genotyping array analysis showed the polymorphic SNPs with take-all resistance from P. huashanica alien introgression were mainly distributed on the chromosome 2A. Genotyping of the F₂ population using the KASP marker mapped a major QTL in an interval of 68.8–70.1 Mb on 2AS. Sixty-two genes were found in the target interval of the Chinese Spring reference genome sequence. According to the functional annotation of genes, two protein genes that can improve the systematic resistance of plant roots were predicted as candidate genes. The development of wheat-P. huashanica introgression line H148 and the resistant QTL mapping information are expected to provide some valuable references for the fine mapping of disease-resistance gene and development of take-all resistant varieties through molecular marker-assisted selection.

Key words: wheat , Psathyrostachys huashanica Keng , take-all , genetic analysis , quantitative trait loci

. 小麦-华山新麦草渗入系H148中全蚀病抗性遗传位点的鉴定[J]. Journal of Integrative Agriculture, 2021, 20(12): 3101-3113.

BAI Sheng-sheng, ZHANG Han-bing, HAN Jing, WU Jian-hui, LI Jia-chuang, GENG Xing-xia, LÜ Bo-ya, XIE Song-feng, HAN De-jun, ZHAO Ji-xin, YANG Qun-hui, WU Jun, CHEN Xin-hong . Identification of genetic locus with resistance to take-all in the wheat-Psathyrostachys huashanica Keng introgression line H148[J]. Journal of Integrative Agriculture, 2021, 20(12): 3101-3113.

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