A rice geranylgeranyl reductase is essential for chloroplast development

doi:10.1016/S2095-3119(20)63324-X

Journal of Integrative Agriculture ›› 2021, Vol. 20 ›› Issue (10): 2592-2600.DOI: 10.1016/S2095-3119(20)63324-X

所属专题：水稻遗传育种合辑Rice Genetics · Breeding · Germplasm Resources

收稿日期:2020-02-07 出版日期:2021-10-01 发布日期:2021-08-09

A rice geranylgeranyl reductase is essential for chloroplast development

LIU Xi, YI Xin, YANG Yan-rong, HUANG Qian-qian

Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection/Jiangsu Key Laboratory for Eco-Agricultural Biotechnology Around Hongze Lake, Huaiyin Normal University, Huai’an 223300, P.R.China

Received:2020-02-07 Online:2021-10-01 Published:2021-08-09
Contact: Correspondence LIU Xi, Tel: +86-517-83525991, E-mail:1240623244@qq.com
Supported by:
This work was supported by the National Natural Science Foundation of China (31900388), the Natural Science Foundation of Jiangsu Province, China (BK20191055) and the Natural Science Foundation of Colleges of Jiangsu Province, China (19KJB180009).

摘要/Abstract

摘要：

叶绿体对植物光合作用和生长是必要的。许多基因被鉴定参与调控植物叶绿体发育。然而，在分子水平上这些基因如何调控叶绿体生物合成是未知的。在本研究中，我们分离了一个色素缺乏的突变体ygl2。YGL2编码一个牻牛儿基牻牛儿基还原酶。YGL2的第三外显子有一个碱基突变（T1361G），导致编码产物发生错义突变（L454R）。透射电镜显示，突变体ygl2的叶绿体发育受损。在突变体ygl2中，质体编码基因的表达水平发生显著变化。此外，通过酵母双杂交分析我们发现YGL2与RNA编辑因子MORF8互作。

Abstract: Chloroplasts are essential for plant photosynthesis and growth. Many genes have been identified that regulate plant chloroplast development. However, it is not known at a molecular level how these genes regulate chloroplast biogenesis. In this study, we isolated a mutant ygl2 (yellow-green leaf2) that exhibited a pigment-defective phenotype. YGL2 encodes a geranylgeranyl reductase, and in mutant ygl2, there was a single base change (T1361G) located in the third exon of YGL2 that resulted in a missense mutation (L454R) in the encoded product. Transmission electron microscopy revealed that chloroplast development was impaired in the ygl2 mutant. The expression levels of plastid-encoded genes were significantly altered in the ygl2 mutant. Furthermore, in a yeast two-hybrid assay, we found that YGL2 interacted with the RNA editing factor MORF8.

Key words: rice (Oryza sativa L.) , chloroplast , YGL2 , geranylgeranyl reductase

. [J]. Journal of Integrative Agriculture, 2021, 20(10): 2592-2600.

LIU Xi, YI Xin, YANG Yan-rong, HUANG Qian-qian. A rice geranylgeranyl reductase is essential for chloroplast development[J]. Journal of Integrative Agriculture, 2021, 20(10): 2592-2600.

参考文献

Barkan A, Goldschmidt-Clermont M. 2000. Participation of nuclear genes in chloroplast gene expression. Biochimie, 82, 559–572.
Bentolila S, Heller W P, Sun T, Babina A M, Friso G, van Wijk K J, Hanson M R. 2012. RIP1, a member of an Arabidopsis protein family, interacts with the protein RARE1 and broadly affects RNA editing. Proceedings of the National Academy of Sciences of the United States of America, 109, 1453–1461.
Chen F, Dong G, Ma X, Wang F, Zhang Y, Xiong E, Wu J, Wang H, Qian Q, Wu L, Yu Y. 2018. UMP kinase activity is involved in proper chloroplast development in rice. Photosynthesis Research, 137, 53–67.
Chu D, Wei L. 2019. The chloroplast and mitochondrial C-to-U RNA editing in Arabidopsis thaliana shows signals of adaptation. The Plant Direct, 3, e00169.
Corneille S, Lutz K, Maliga P. 2000. Conservation of RNA editing between rice and maizeplastids: Are most editing events dispensable? Molecular Genetics and Genomics, 264, 419–424.
Cui X, Wang Y, Wu J, Han X, Gu X, Lu T, Zhang Z. 2018. The RNA editing factor DUA1 is crucial to chloroplast development at low temperature in rice. New Phytologist, 221, 834–849.
Hammani K, Okuda K, Tanz S K, Chateigner-Boutin A L, Shikanai T, Small I. 2009. A study of new Arabidopsis chloroplast RNA editing mutants reveals general features of editing factors and their target sites. The Plant Cell, 21, 3686–3699.
Kimura E, Abe T, Murata K, Kimura T, Otoki Y, Yoshida T, Miyazawa T, Nakagawa K. 2018. Identification of OsGGR2, a second geranylgeranyl reductase involved in α-tocopherol synthesis in rice. Scientific Reports, 8, 1870.
Li S, Mason C E. 2014. The pivotal regulatory landscape of RNA modifications. Annual Review of Genomics and Human Genetics, 15, 127–150.
Li X J, Gu W, Sun S L, Chen Z L, Chen J, Song W B, Zhao H M, Lai J S. 2018. Defective kernel 39 encodes a PPR protein required for seed development in maize. Journal of Integrative Plant Biology, 60, 45–64.
Liu X, Lan J, Huang Y S, Cao P H, Zhou C L, Ren Y K, He N Q, Liu S J, Tian Y L, Nguyen T, Jiang L, Wan J M. 2018. WSL5, a pentatricopeptide repeat protein, is essential for chloroplast biogenesis in rice under cold stress. Journal of Experiment Botany, 69, 3949–3961.
Jarvis P, López-Juez E. 2013. Biogenesis and homeostasis of chloroplasts and other plastids. Nature Reviews Molecular Cell Biology, 14, 787–802.
Niu M, Wang Y, Wang C, Lyu J, Wang Y, Dong H, Long W, Wang D, Kong W, Wang L, Guo X, Sun L, Hu T, Zhai H, Wang H, Wan J M. 2017. ALR encoding dCMP deaminase is critical for DNA damage repair, cell cycle progression and plant development in rice. Journal of Experiment Botany, 68, 5773–5786.
Pfalz J, Liere K, Kandlbinder A, Dietz K J, Oelmüller R. 2006. pTAC2, –6, and –12 are components of the transcriptionally active plastid chromosome that are required for plastid gene expression. The Plant Cell, 18, 176–197.
Pogson B J, Ganguly D, Albrecht-Borth V. 2015. Insights into chloroplast biogenesis and development. Biochimica et Biophysica Acta, 1847, 1017–1024.
Porra R J, Thompson W A, Kriedemann P E. 1989. Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: Verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochimica et Biophysica Acta (Bioenergetics), 975, 384–394.
Pyo Y J, Kwon K C, Kim A, Cho M H. 2013. Seedling lethal1, a pentatricopeptide repeat protein lacking an E/E+ or DYW domain in Arabidopsis, is involved in plastid gene expression and early chloroplast development. Plant Physiology, 163, 1844–1858.
Sakamoto W, Miyagishima S Y, Jarvis P. 2008. Chloroplast biogenesis: Control of plastid development, protein import, division and inheritance. Arabidopsis Book, 6, e0110.
Steiner S, Schröter Y, Pfalz J, Pfannschmidt T. 2011. Identification of essential subunits in the plastid-encoded RNA polymerase complex reveals building blocks for proper plastid development. Plant Physiology, 157, 1043–1055.
Takenaka M, Verbitskiy D, Zehrmann A, Brennicke A. 2010. Reverse genetic screening identifies five E-class PPR proteins involved in RNA editing in mitochondria of Arabidopsis thaliana. Journal of Biology Chemistry, 285, 27122–27129.
Tanaka R, Rothbart M, Oka S, Takabayashi A, Takahashi K, Shibata M, Myouga F, Motohashi R, Shinozaki K, Grimm B, Tanaka A. 2010. LIL3, a light-harvesting-like protein, plays an essential role in chlorophyll and tocopherol biosynthesis.Proceedings of the National Academy of Sciences of the United States of America, 107, 16721–16725.
Takenaka M, Zehrmann A, Verbitskiy D, Kugelmann M, Hartel B, Brennicke A. 2012. Multiple organellar RNA editing factor (MORF) family proteins are required for RNA editing in mitochondria and plastids of plants. Proceedings of the National Academy of Sciences of the United States of America, 109, 5104–5109.
Wang Y, Ren Y, Zhou K, Liu L, Wang J, Xu Y, Zhang H, Zhang L, Feng Z, Wang L, Ma W, Wang Y, Guo X, Zhang X, Lei C, Cheng Z, Wan J M. 2017. WHITE STRIPE LEAF4 encodes a novel P-type PPR protein required for chloroplast biogenesis during early leaf development. Frontier in Plant Science, 8, 1116.
Wang Y, Wang C, Zheng M, Lyu J, Xu Y, Li X, Niu M, Long W, Wang D, Wang H, Terzaghi W, Wang Y, Wan J M. 2016. WHITE PANICLE1, a val-tRNA synthetase regulating chloroplast ribosome biogenesis in rice, is essential for early chloroplast development. Plant Physiology, 170, 2110–2123.
Zhang Z, Cui X, Wang Y, Wu J, Gu X, Lu T. 2017. The RNA editing factor WSP1 is essential for chloroplast development in rice. Molecular Plant, 10, 86–98.
Zhou W, Cheng Y, Yap A, Chateigner-Boutin A L, Delannoy E, Hammani K, Small I, Huang J. 2009. The Arabidopsis gene YS1 encoding a DYW protein is required for editing of rpoB transcripts and the rapid development of chloroplasts during early growth. The Plant Journal, 58, 82–96.
Zhou Y, Gong Z, Yang Z, Yuan Y, Zhu J, Wang M, Yuan F, Wu S, Wang Z, Yi C, Xu T, Ryom M, Gu M, Liang G H. 2013. Mutation of the light-induced yellow leaf 1 gene, which encodes a geranylgeranyl reductase, affects chlorophyll biosynthesis and light sensitivity in rice. PLoS ONE, 8, e75299.

A rice geranylgeranyl reductase is essential for chloroplast development

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