Breeding of CMS maintainer lines through anther culture assisted by high-resolution melting-based markers

doi:10.1016/S2095-3119(20)63179-3

Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (12): 2965-2973.DOI: 10.1016/S2095-3119(20)63179-3

所属专题：水稻遗传育种合辑Rice Genetics · Breeding · Germplasm Resources

收稿日期:2019-08-05 出版日期:2020-12-01 发布日期:2020-11-19

Breeding of CMS maintainer lines through anther culture assisted by high-resolution melting-based markers

WANG Ping^{1, 2*}, BAI Yu-lu^2*, WANG Min-xia², HU Bin-hua², PU Zhi-gang², ZHANG Zhi-yong², ZHANG Qiong², XU Deng-wu², LUO Wen-long³, CHEN Zhi-qiang¹

¹ National Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou 510642, P.R.China
² Institute of Biological and Nuclear Technology, Sichuan Academy of Agricultural Sciences, Chengdu 610061, P.R.China
³ Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, P.R.China

Received:2019-08-05 Online:2020-12-01 Published:2020-11-19
Contact: Correspondence CHEN Zhi-qiang, Tel/Fax: +86-20-85283237, E-mail: chenlin@scau.edu.cn
About author:WANG Ping, E-mail: wangping2014@163.com; * These authors contributed equally to this study.
Supported by:
This research was supported by the National Key Technology Research and Development Program of China (2016YFD0102102), the Sichuan Innovation Ability Rising Government Financial Project, China (2016ZYPZ-001) and the Sichuan Rice Breeding Project for Tackling Key Problems, China (2016NYZ0028-1-1).

摘要/Abstract

Abstract:

The integrated use of molecular marker-assisted selection (MAS) and anther culture has potential to significantly increase efficiency in plant breeding; however, reports on this kind of practical use are very limited. In the present study, we report the development of cytoplasmic male sterile (CMS) maintainers with aroma, disease resistance and red-brown hulls, as an example of integration of MAS and anther culture in rice breeding. A high-resolution melting (HRM)-based functional molecular marker was developed for the red-brown hull trait caused by a unique mutation (rbh1) in OsCAD2. Functional molecular markers for genes of rice blast resistance (Pi2), aroma (fgr) and red-brown hull (rbh1) were used for precise genotyping of individual plants in the BC₁ and BC₂F₂ populations derived from a cross between CMS maintainers Huaxiang B (pi2–/rbh1–/fgr–) and Rong 3B (Pi2+/RBH1+/Fgr+). A total of 89 doubled haploid (DH) lines were generated from selected BC₂F₂ plants (Pi2+/rbh1–/fgr–) by anther culture. Seven DH lines were subsequently selected as the potential new CMS maintainers based on their overall performance and high resistance to blast. Our study demonstrated that integration of MAS and anther culture significantly accelerated the development of CMS maintainers with multiple stacked genes.

Key words: breeding efficiency , maintainer of cytoplasmic male sterility , gene pyramiding , molecular marker-assisted selection

. [J]. Journal of Integrative Agriculture, 2020, 19(12): 2965-2973.

WANG Ping, BAI Yu-lu, WANG Min-xia, HU Bin-hua, PU Zhi-gang, ZHANG Zhi-yong, ZHANG Qiong, XU Deng-wu, LUO Wen-long, CHEN Zhi-qiang. Breeding of CMS maintainer lines through anther culture assisted by high-resolution melting-based markers[J]. Journal of Integrative Agriculture, 2020, 19(12): 2965-2973.

参考文献

Amarawathi Y, Singh R, Singh A K, Singh V P, Mohapatra T, Sharma T R, Singh N K. 2008. Mapping of quantitative trait loci for basmati quality traits in rice (Oryza sativa L.). Molecular Breeding, 21, 49–65.
Bradbury L M, Fitzgerald T L, Henry R J, Jin Q, Waters D L. 2005. The gene for fragrance in rice. Plant Biotechnology Journal, 3, 363–370.
Brar D, Khush G. 2006. Cytogenetic manipulation and germplasm enhancement of rice (Oryza sativa L.). In: Genetic Resources, Chromosome Engineering and Crop Improvement. CRC Press, Boca Raton, USA. pp. 115–158.
Chen C C, Tsay H S, Huang C R. 1991. Factors affecting androgenesis in rice (Oryza sativa L.). Biotechnology in Agriculture & Forestry, 14, 192–215.
Deng Q Y. 2005. Breeding of the PTGMS line Y58S with wide adaptability in rice. Hybrid Rice, 20, 15–18. (in Chinese)
Dong R X, Wang H F, Dong L F, Zhou P, Tu S H, You Q R, Liao F L, Huang T X. 2017. Improving the rice blast resistance for a CMS line of rice Zhenda A and its hybrids using molecular marker-assistant selection. Journal of Plant Genetic Resources, 18, 573–586. (in Chinese)
Duan M, Xie L J, Huang S J, Pan X B. 2016. Research progress on the breeding of indica sterile line in rice. China Rice, 22, 25–29. (in Chinese)
Ganopoulos I, Argiriou A, Tsaftaris A. 2011. Adulterations in Basmati rice detected quantitatively by combined use of microsatellite and fragrance typing with high resolution melting (HRM) analysis. Food Chemistry, 129, 652–659.
Germana M A. 2011. Gametic embryogenesis and haploid technology as valuable support to plant breeding. Plant Cell Reports, 30, 839–857.
Gundry C N, Vandersteen J G, Reed G H, Pryor R J, Chen J, Wittwer C T. 2003. Amplicon melting analysis with labeled primers: A closed-tube method for differentiating homozygotes and heterozygotes. Clinical Chemistry, 49, 396–406.
He L B, Cao L Y, Qian Q, Chen S H. 2001. The prospects of hybrid rice seed production by using rice chaff colour marker. Acta Agriculturae Zhejiangensis, 13, 357–360. (in Chinese)
He T, Yang Y, Tu S B, Yu M Q, Li X F. 2006. Selection of inter specific hybrids for anther culture of indica rice. Plant Cell, Tissue and Organ Culture, 86, 271–277.
Hirano K, Aya K, Kondo M, Okuno A, Morinaka Y, Matsuoka M. 2012. OsCAD2 is the major CAD gene responsible for monolignol biosynthesis in rice culm. Plant Cell Reports, 31, 91–101.
Hofinger B J, Jing H C, Hammond-Kosack K E, Kanyuka K. 2009. High-resolution melting analysis of cDNA-derived PCR amplicons for rapid and cost-effective identification of novel alleles in barley. Theoretical and Applied Genetics, 119, 851–865.
IRRI (International Rice Research Institute). 2013. Standard Evaluation System for Rice. Manila, Philippines. pp. 18–19.
Jin M N, Chen Z F, Qiu S J, Chen H, Xie G, Li Z X, Tang X Y. 2018. Development and application of 2 specific molecular markers for the disease resistance and epithelial resistance of the people and the system. Journal of Agricultural Biotechnology, 26, 365–373. (in Chinese)
Koressaar T, Remm M. 2007. Enhancements and modifications of primer design program primer3. Bioinformatics, 23, 1289–1291.
Koshiba T, Murakami S, Hattori T, Mukai M, Kishi-Kaboshi M, Miyao A, Hirochika H, Suzuki S, Sakamoto M, Umezawa T. 2013. CAD2 deficiency causes both brown midrib and gold hull and internode phenotypes in Oryza sativa L. cv. Nipponbare. Plant Biotechnology, 30, 365–373.
Li S, Liu S M, Fu H W, Huang J Z, Shu Q Y. 2018. High-resolution melting-based TILLING of γ ray-induced mutations in rice. Journal of Zheijang University (Science B), 19, 620–629.
Li Y D, Chu Z Z, Liu X G, Jing H C, Liu Y G, Hao D Y. 2010. A cost-effective high-resolution melting approach using the evagreen dye for DNA polymorphism detection and genotyping in plants. Journal of Integrative Plant Biology, 52, 1036–1042.
Liu W G, Wang F, Liu Z R, Huang H J, Zhu M S, Li J H, Liao Y L, Fu C Y, Liu D L. 2015. Breeding and utilization of blast-resistant CMS line Anfeng A in rice. Hybrid Rice, 30, 5–7. (in Chinese)
Luo W L, Huang M, Guo T, Xiao W, Wang J, Yang G, Liu Y, Wang H, Chen Z, Zhuang C. 2017. Marker-assisted selection for rice blast resistance genes Pi2 and Pi9 through high-resolution melting of a gene-targeted amplicon. Plant Breeding, 136, 67–73.
Luo W L, Guo T, Yang Q Y, Wang H, Liu Y Z, Zhu X Y, Chen Z Q. 2014. Stacking of five favorable alleles for amylase content, fragrance and disease resistance into elite lines in rice (Oryza sativa) by using four HRM-based markers and a linked gel-based marker. Molecular Breeding, 34, 805–815.
Luo W L, Guo T, Zhou D H, Chen H Y, Wang H, Chen Z Q, Liu Y Z. 2013. Analysis of rice genotypes rice Wx and fgr by HRM-based functional marker. Journal of Hunan Agricultural University (Natural Science), 39, 597–603. (in Chinese)
Luo X H, Qiu Z Z, Li R H. 1992. Pei’ai64 S, a dual-purpose sterile line whose sterility is induced by low critical temperature. Hybrid Rice, 27–29. (in Chinese)
Mei C S, Zhang J Y, Wu G N. 1988. Improving regeneration rate of anther culture in indica rice. Jiangsu Journal of Agricultural Sciences, 16, 45–48. (in Chinese)
Mishra R, Rao G J N. 2016. In vitro androgenesis in rice: Advantages, constraints and future prospects. Rice Science, 23, 57.
Murashige T, Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15, 473–497.
Niizeki H, Oono K. 1968. Induction of haploid rice plant from anther culture. Proceedings of the Japan Academy, 44, 554–557.
Ookawa T, Inoue K, Matsuoka M, Ebitani T, Takarada T, Yamamoto T, Ueda T, Yokoyama T, Sugiyama C, Nakaba S, Funada R, Kato H, Kanekatsu M, Toyota K, Motobayashi T, Vazirzanjani M, Tojo S, Hirasawa T. 2014. Increased lodging resistance in long-culm, low-lignin gh2 rice for improved feed and bioenergy production. Scientific Reports, 4, 65–67.
Palanisamy D, Marappan S, Ponnuswamy R D, Prabhu S M, Rajaguru B, Subramanian V. 2019. Accelerating hybrid rice breeding through the adoption of doubled haploid technology for R-line development. Biologia, 10, 1259–1269.
Pan R S, Zheng B W, Mao D M, Guan H Z, Chen Z W, Lin L H, Zhao J Z, Zhou Y C. 2009. Breeding of quality rice CMS line Hua2 A by anther culture. Hybrid Rice, 24, 12–15. (in Chinese)
Serrat X, Cardona M, Gil J, Brito A M, Moysset L, Nogués S, Lalanne E. 2014. A Mediterranean japonica rice (Oryza sativa) cultivar improvement through anther culture. Euphytica, 195, 31–44.
Tan Y Y, Fu H W, Zhao H J, Lu S, Fu J J, Li Y F, Cui H R, Shu Q Y. 2013. Functional molecular markers and high-resolution melting curve analysis of low phytic acid mutations for marker-assisted selection in rice. Molecular Breeding, 31, 517–528.
Tara D S. 2010. Indica rice anther culture: Can the impasse be surpassed? Plant Cell Tissue and Organ Culture, 100, 1–11.
Trejo T G, Amaya U M, Morales G S, Sánchez A D S, Bonfil B M, Rodríguez-Monroy M, Jiménez-Aparicio A. 2002. The effects of cold-pretreatment, auxins and carbon source on anther culture of rice. Plant Cell Tissue and Organ Culture, 71, 41–46.
Tu S H, Zhou P, Zheng Y, Dong R X, Zhang S J, Huang T X, Zheng J T. 2015. Breeding blast-resistant male sterile line of three line hybrid rice by Pi25 gene marker assisted selection. Molecular Plant Breeding, 13, 1911–1917. (in Chinese)
Wang H, Zhang Y, Sun L, Meng S, Peng X U, Weixun W U, Cheng S, Cao L, Shen X. 2017. Map-based cloning of OsCAD2 regulating golden hull and internode in rice. Chinese Journal of Rice Science, 31, 465–474. (in Chinese)
Wang P, Zhang Z X, Xiang Y W, Wang M X, Cai P Z, Zhang Z Y, Lin Y. 2012. Breeding and utilization of indica restorer line Chuanhui 1618 with good grain quality and high combining ability. Hybrid Rice, 27, 20–22. (in Chinese)
Wittwer C T, Reed G H, Gundry C N, Vandersteen J G, Pryor R J. 2003. High-resolution genotyping by amplicon melting analysis using LC Green. Clinical Chemistry, 49, 853–860.
Yang Z P. 1997. Breeding of widely compatible restorer lines in rice (Oryza sativa L.) through anther culture. Euphytica, 95, 253–258.
Yuan L P. 2016. Research and development of hybrid rice in China. Science & Technology Review, 34, 64–65. (in Chinese)
Zhang K W, Qian Q, Huang Z J, Wang Y Q, Li M, Hong L L, Zeng D L, Gu M H, Chu C C, Cheng Z K. 2006. Gold hull and internode2 encodes a primarily multifunctional cinnamyl-alcohol dehydrogenase in rice. Plant Physiology, 140, 972–983.
Zhang Z X, Zhang Z Y, Xiang Y W, Wang P, Cai P Z, Pu Z G. 2009. Breeding and utilization of aromatic CMS line Huaxiang A with good grain quality and a salmon pink glume marker in rice. Hybrid Rice, 24, 15–16. (in Chinese)
Zhu M S, Huang H J, Wang F, Liu Z R, Liu W G, Liao Y L, Li J H, Chen J W. 2013. Taifengyou 55, a new indica hybrid rice combination with weak photosensitivity, fine grain quality and blast resistance. Hybrid Rice, 28, 76–77. (in Chinese)
Zhu S S, Shi W J, Zhang Y S, Zhu Y, Zhang D S, Huang P Y, Wan J M. 2010. Breeding of indica rice CMS line H22A with medium amylose content by molecular marker-aided selection. Hybrid Rice, 25, 9–12. (in Chinese)

Breeding of CMS maintainer lines through anther culture assisted by high-resolution melting-based markers

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics